The spore antigens of and the butyric group were compared. By spore agglutination and fluorescent-antibody technique (FAT) the 69 strains of were divided into two types: serum raised against type I (66 strains) reacted with all strains of this species but showed no cross reaction with any of the three types of ; serum raised against type II (three strains) did not react with strains of type I but showed cross reaction with all the 66 strains of type I. Thus, by antigenic analysis, spores of type I were found to possess two components designated E and F; E was a type-specific component whilst F was shared by strains of type II. In addition, strains of type II possessed a second component, G, which was shared by strains of type I. There was no cross reaction between the precipitinogens of the spores of the two species. Five strains of formed a homogeneous group as judged by spore agglutination, FAT and precipitation reactions. There was no cross reaction with any of the other proteolytic species studied. Five butyric strains also formed a homogeneous group. Two antigenic components were therefore assigned to the spore antigens of the two groups: J, for and K, for the butyric strains.


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