1887

Abstract

SUMMARY

A rabbit was immunised with a heat-killed vaccine prepared from strain 1142 of The HA titres of the antisera against a surface-polysaccharide antigen extracted from the homologous strain were highest in the 2nd–4th weeks after immunisation and subsequently declined. Only a minor amount of 2-ME-resistant antibody was formed, and this at a late stage of the reaction. After a booster injection of the vaccine at the 10th week, there was a significantly greater HA-antibody response and a greater amount of 2-ME-resistant antibody was formed.

The relative passive-protective activity of the sera in mice corresponded to their content of 2-ME-sensitive HA antibody. The protective activity of the sera was 2-ME sensitive and was not removed by absorption with anti-rabbit IgG goat serum. Further, 280 μg of IgM-rich serum fraction obtained by sucrose-density-gradient ultracentrifugation passively protected against homologous challenge infection in mice, but even 910 μg of IgG-rich fraction did not. These results indicated that the protective antibody was of the IgM class.

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/content/journal/jmm/10.1099/00222615-11-4-371
1978-11-01
2022-09-25
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References

  1. Ekstedt R. D., Yoshida K. 1969; Immunity to staphylococcal infection in mice: effect of living versus killed vaccine, role of circulating antibody, and induction of protection-inducing antigen(s) in vivo. J. Bact 100:745
    [Google Scholar]
  2. Fisher M. W., Delvin H. B., Erlandson A. L. 1963; A new staphylococcal antigen: its preparation and immunizing activity against experimental infections. Nature, Lond 199:1074
    [Google Scholar]
  3. Morse S. I. 1962; Isolation and properties of a surface antigen of Staphylococcus aureus. J. exp. Med 115:295
    [Google Scholar]
  4. Rogers D. E. 1962; Staphylococci and man. J. Am. Med. Ass 181:38
    [Google Scholar]
  5. Yoshida K., Ekstedt R. D. 1968; Antibody response to Staphylococcus aureus in rabbits: sequence of immunoglobulin synthesis and its correlation with passive protection in mice. J. Bact 96:1540
    [Google Scholar]
  6. Yoshida K., Icpdman Y., Ohtomo T. 1976; Relation of antiphagocytic activity of a strain of Staphylococcus epidermidis to the induction of resistance in mice. Zentbl. Bakt. ParasitKde I Abt. Orig. A, Suppl 5819
    [Google Scholar]
  7. Yoshida K., Mizunari S., Hattori H. 1964; Immunological studies on “ surface antigen ” extracted from Staphylococcus aureus. I. Isolation of the antigen and properties of the immune rabbit serum. Jap. J. Microbiol 8:67
    [Google Scholar]
  8. Yoshida K., Smith M. R., Naito Y. 1971; Demonstration of serologically different capsular types among strains of Staphylococcus aureus by the serum-soft agar technique. Infect. Immun 3:535
    [Google Scholar]
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