1887

Abstract

SUMMARY

Immune electronmicroscopy (IEM) was used to identify human picorna-viruses rapidly and to differentiate enteroviruses from rhinoviruses. Human sera, diluted 10- to 50-fold beyond the neutralisation endpoints for homologous virus, readily agglutinated C-type antigens of seven human picornaviruses. Human sera did not react by IEM with a control animal picornavirus. By IEM after acid treatment, differentiation of a human enterovirus from a human rhinovirus was possible. There was an excellent correlation between the results of IEM and immunodiffusion (ID) tests for the presence of antibody to human picornavirus group antigens. By ID, only one of 21 human sera reacted with one of six animal picornaviruses. Immune electronmicroscopy appears to be a sensitive and simple technique for the detection of picornavirus C-type antigens, and may be useful for identifying viruses belonging to groups comprising many serotypes and sharing a common group antigen.

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1977-05-01
2022-05-27
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References

  1. Almeida J. D., Waterson A. P. 1969; The morphology of virus-antibody interactions. Adv. Virus Res 15:307
    [Google Scholar]
  2. Erson N., Doane F. W. 1973; Specific identification of enteroviruses by immuno-electron microscopy using a serum-in-agar diffusion method. Can. J. Microbiol 19:585
    [Google Scholar]
  3. Bayer M. E., Blumberg B. S., Werner B. 1968; Particles associated with Australia antigen in the sera of patients with leukaemia, Down’s syndrome and hepatitis. Nature, Lond 218:1057
    [Google Scholar]
  4. Chaudhary R. K., Kennedy D. A., Westwood J. C. N. 1971; Serological cross-reactivity within the picornaviruses as studied by electron microscopy. Can. J. Microbiol 17:477
    [Google Scholar]
  5. Conant R. M., Somerson N. L., Hamparian V. V. 1968; Plaque formation by rhino-viruses. Proc. Soc. exp. Biol. Med 128:51
    [Google Scholar]
  6. Conant R. M., Hamparian V. V. 1968; Rhinoviruses: basis for a numbering system. I. HeLa cells for propagation and serologic procedures. J. Immun 100:107
    [Google Scholar]
  7. Doane F. W. 1974; Identification of viruses by immunoelectron microscopy. In Viral immunodiagnosis edited by Kurstak E., Morisset R. New York: p 237
    [Google Scholar]
  8. Feinstone S. M., Kapikian A. Z., Purcell R. H. 1973; Hepatitis A: detection by immune electron microscopy of a viruslike antigen associated with acute illness. Science, N. Y 182:1026
    [Google Scholar]
  9. Hampil B., Melnick J. L. 1969; Method for testing virus stocks for viral coantaminants. Appl. Microbiol 17:17
    [Google Scholar]
  10. Hughes J. H., Thomas D. C., Hamparian V. V. 1973; Acid lability of rhinovirus type 14; effect of p H, time, and temperature. Proc. Soc. exp. Biol. Med 144:555
    [Google Scholar]
  11. Hughes J. H., Chema S., Lin N., Conant R. M., Hamparian V. V. 1974; Acid lability of rhinoviruses: loss of C and D antigenicity after treatment at pH 3.0. J. Immun 112:919
    [Google Scholar]
  12. Hummeler K., Erson T. F., Brown R. A. 1962; Identification of poliovirus particles of different antigenicity by specific agglutination as seen in the electron microscope. Virology 16:84
    [Google Scholar]
  13. Kapikian A. Z., Almeida J. D., Stott E. J. 1972; Immune electron microscopy of rhinoviruses. J. Virol 10:142
    [Google Scholar]
  14. Kapikian A. Z., Wyatt R. G., Dolin R., Thornhill T. S., Kalica A. R., Chanock R. M. 1972; Visualization by immune electron microscopy of a 27-nm particle associated with acute infectious nonbacterial gastroenteritis. J. Virol 10:1075
    [Google Scholar]
  15. Kapikian A. Z., James H. D. Jr, Kelly S. J., Vaughn A. L. 1973; Detection of coronavirus strain 692 by immune electron microscopy. Infect. Immun 7:111
    [Google Scholar]
  16. Kapikian A. Z., Kim H. W., Wyatt R. G., Rodriguez W. J., Ross S., Cline W. L., Parrott R. H., Chanock R. M. 1974; Reoviruslike agent in stools: association with infantile diarrhea and development of serologic tests. Science, N. Y 185:1049
    [Google Scholar]
  17. Reeves J. D., Mayor H. D. 1973; The effects of hydrogen ions on the morphology and infectivity of rhino virions. Arch. ges. Virusforsch 40:325
    [Google Scholar]
  18. Schmidt N.J., Dennis J., Lennette E. H., Ho H. H., Shinomoto T. T. 1965; Antibody responses of rhesus (Macaca mulatto) monkeys experimentally infected with coxsackie-viruses of group B and group A, type 9. I. Antibody responses within the coxsackievirus group. J. Immun 95:54
    [Google Scholar]
  19. Valters W. A., Boehm L. G., Edwards E. A., Rosenbaum M. J. 1975; Detection of adenovirus in patient specimens by indirect immune electron microscopy. J. clin. Microbiol 1:472
    [Google Scholar]
  20. Vassall J. H. II, Ray C. G. 1974; Serotyping of adenoviruses using immune electron microscopy. Appl. Microbiol 28:623
    [Google Scholar]
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