1887

Abstract

Summary

Thirty-seven strains of isolated from bacteraemia and 40 faecal strains isolated from healthy individuals were O serogrouped and investigated for the production of colicins, haemolysin (Hly), cytotoxic necrotising factor (CNF), lethal activity for mice, the expression of P fimbriae, mannose-resistant (MRHA) and mannose-sensitive (MSHA) haemagglutination, and relative cell surface hydrophobicity. Virulence factors significantly associated with bacteraemic strains were: serogroups O2, O4, O6, O7, O8 and O75 (54% 10%, p<0.001), production of Hly (32% 8%, p<0.02) and CNF (38% 10%, p<0.01), expression of P fimbriae (27% 5%, p<0.02), MRHA types III, IVa and IVb (51% 8%, p<0.001), and possession of a moderate cell surface hydrophobic charge (35% 13%, p<0.05). Virulence factors were strongly associated with strains expressing defined MRHA types. Thus, all strains belonging to MRHA types III and IVa were toxigenic, whereas only 11% of strains belonging to MRHA types IVb, V or VI were toxigenic (p<0.001). Virulence factors were concentrated in strains belonging to O serogroups usually found in that cause extra-intestinal infections, especially in strains of O4 and O6 groups. The most interesting result of this study was that all 12 P-fimbriate strains expressed the MRHA type IVa and 11 of them synthesised CNF.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00221317-31-3-175
1990-03-01
2022-01-28
Loading full text...

Full text loading...

/deliver/fulltext/jmm/31/3/medmicro-31-3-175.html?itemId=/content/journal/jmm/10.1099/00221317-31-3-175&mimeType=html&fmt=ahah

References

  1. Alonso M, Blanco J, Blanco M, GonzSlez E A. 1987; Frequent production of toxins by Escherichia coli strains isolated from human urinary tract infections : relation with haemagglutination. FEMS Microbiology Letters 48:391–396
    [Google Scholar]
  2. Bisicchia R, Ciammarughi R, Caprioli A, Falbo V, Ruggeri F M. 1985; Toxin production and haemagglutination in strains of Escherichia coli from diarrhoea in Brescia, Italy. Journal of Hygiene 95:353–361
    [Google Scholar]
  3. Blanco J, Gonzblez E A, Anadon R. 1985; Colonization antigens and haemagglutination patterns of human Escherichia coli. European Journal of Clinical Microbiology 4:316–326
    [Google Scholar]
  4. Blanco J, GonzSlez E A, Blanco M, Alonso M P, Barbadillo M J. 1988a; Toxins and serotypes of faecal non-enterotoxigenic and non-enteropathogenic Escherichia coli strains causing mannose-resistant haemagglutination : relation with haemagglutination patterns. Zentralblatt fur Bakteriologie Mikrobiologie und Hygiene Originale A 269:43–55
    [Google Scholar]
  5. Blanco J, Gonzblez E A, Garcia S, Blanco M, Regueiro B, Bernsrdez I. 1988b; Production of toxins by Escherichia coli strains isolated from calves with diarrhoea in Galicia (North-westem Spain). Veterinary Microbiology 18:297–311
    [Google Scholar]
  6. Brauner A, Leissner M, Wretlind B, Julander I, Svenson S B, Kallenius G. 1985; Occurrence of P-fimbriated Escherichia coli in patients with bacteremia. European Journal of Clinical Microbiology 4:566–569
    [Google Scholar]
  7. Brauner A. 1987; The use of biochemical markers, serotype and fimbriation in the detection of Escherichia coli clones.. Journal of General Microbiology 133:2825–2834
    [Google Scholar]
  8. Caprioli A, Falbo V, Roda L G, Ruggeri F M, Zona C. 1983; Partial purification and characterization of an Escherichia coli toxic factor that induces morphological cell alterations.. Infection and Immunity 39:1300–1306
    [Google Scholar]
  9. Caprioli A. 1987; Cytotoxic necrotizing factor production by hemolytic strains of Escherichia coli causing extraintestinal infections. Journal of Clinical Microbiology 25:146–149
    [Google Scholar]
  10. Cross A S, Gemski P, Sadoff J C, Orskov F, Orskov I. 1984; The importance of the K1 capsule in invasive infections caused by Escherichia coli. Journal of Infectious Diseases 149:184–193
    [Google Scholar]
  11. Czirόk E. 1985; Virulence factors of Escherichia coli. 11. Antigens O4, O6 and O18, haemolysin production and mannose resistant haemagglutinating capacity are closely associated.. Acta Microbiologica Hungarica 149:183–192
    [Google Scholar]
  12. De Rycke J, Guillot J F, Boivin R. 1987; Cytotoxins in nonenterotoxigenic strains of Escherichia coli isolated from feces of diarrheic calves. Veterinary Microbiology 149:137–150
    [Google Scholar]
  13. Domingue G J. 1985; Pathogenic significance of Pfimbriated Escherichia coli in urinary tract infections. Journal of Urology 133:983–989
    [Google Scholar]
  14. Duguid J P, Clegg S, Wilson M I. 1979; The fimbrial and nonfimbrial haemagglutinins of Escherichia coli. Journal of Medical Microbiology 149:213–227
    [Google Scholar]
  15. Evans D J, Evans D G, Young L S, Pitt J. 1980; Hemagglutination typing of Escherichia coli: definition of seven hemagglutination types. Journal of Clinical Microbiology 12:235–242
    [Google Scholar]
  16. Evans D J. 1981; Hemolysin and K antigens in relation to serotype and hemagglutination type of Escherichia coli isolated from extraintestinal infections. Journal of Clinical Microbiology 13:171–178
    [Google Scholar]
  17. Gaastra W, de Graaf F K. 1982; Host-specific fimbrial adhesins of noninvasive enterotoxigenic Escherichia coli strains. Microbiological Reviews 149:129–161
    [Google Scholar]
  18. Gonzblez E A, Blanco J. 1985; Production of cytotoxin VT in enteropathogenic and non-enteropathogenic Escherichia coli strains of porcine origin. FEMS Microbiology Letters 26:129–161
    [Google Scholar]
  19. Gonzhlez E A, Blanco J, Baloda S B, Wadstrom T. 1988; Relative cell surface hydrophobicity of Escherichia coli strains with various recognized fimbrial antigens and without recognized fimbriae. Zentralblatt fur Bakteriologie Mikrobiologie und Hygiene Originale A 269:218–236
    [Google Scholar]
  20. é P A M, Agterberg C M., Jansen W H. 1972127–131
  21. é P A M, Veldkamp J, Jansen W H. 1977; Improved minca medium for the detection of K99 antigen in calf enterotoxigenic strains of Escherichia coli. Infection and Immunity 149:676–678
    [Google Scholar]
  22. Hacker J, Schrettenbrunner A, Schroter G, Duvel H, Schmidt G, Goebel W. 1986; Characterization of Escherichia coli wildtype strains by means of agglutination with antisera raised against cloned P-, S-, and MS-fimbriae antigens, hemagglutination, serotyping and hemolysin production. Zentralblatt fur Bakteriologie Mikrobiologie und Hygiene Originale A 261:216–231
    [Google Scholar]
  23. Johnson J R, Moseley S L, Roberts P L, Stamm W E. 1988; Aerobactin and other virulence factor genes among strains of Escherichia coli causing urosepsis : association with patient characteristics. Infection and Immunity 56:405–412
    [Google Scholar]
  24. Korhonen T K. 1985; Serotypes, hemolysin production, and receptor recognition of Escherichia coli strains associated with neonatal sepsis and meningitis. Infection and Immunity 48:486–491
    [Google Scholar]
  25. Kreger B E, Craven D E, Carling P C, McCabe W R. 1980; Gram-negative bacteremia. 111. Reassessment of etiology, epidemiology and ecology in 612 patients. American Journal of Medicine 68:332–343
    [Google Scholar]
  26. Levine M M. 1987; Escherichia coli that cause diarrhea: enterotoxigenic, enteropathogenic, enteroinvasive, enterohemorrhagic, and enteroadherent. Journal of Infectious Diseases 149:377–389
    [Google Scholar]
  27. Ljungh A, Wadstriim T. 1982; Salt aggregation test for measuring cell surface hydrophobicity of urinary Escherichia coli. European Journal of Clinical Microbiology 149:388–392
    [Google Scholar]
  28. Minshew B H, Jorgensen J, Counts G W, Falkow S. 1978; Association of hemolysin production, hemagglutination of human erythrocytes, and virulence for chicken embryos of extraintestinal Escherichia coli isolates. Infection and Immunity 20:50–54
    [Google Scholar]
  29. Montgomerie J Z, Bindereif A, Neilands J B, Kalmanson G M, Guze L B. 1984; Association of hydroxamate siderophore (aerobactin) with Escherichia coli isolated from patients with bacteremia. Infection and Immunity 46:835–838
    [Google Scholar]
  30. O’Hanley P. 1985; Gal-Gal binding and hemolysin phenotypes and genotypes associated with uropathogenic Escherichia coli. New England Journal of Medicine 313:414–420
    [Google Scholar]
  31. Ørskov F, Ørskov I. 1975; Escherichia coli O : H serotypes isolated from human blood. Prevalence of the K 1 antigen with technical details of 0 and H antigen determination. Acta Pathologica et Microbiologica Scandinavica Section B (Sup pl) 83:585–600
    [Google Scholar]
  32. Ørskov I, Ørskov F. 1985; Escherichia coli in extra-intestinal infections. Journalof Hygiene 95:551–575
    [Google Scholar]
  33. Quackenbush R L, Falkow S. 1979; Relationship between colicin V activity and virulence in Escherichia coli. Infection and Immunity 24:562–564
    [Google Scholar]
  34. Vaisanen V. 1981; Mannose-resistant haemagglutination and P antigen recognition are characteristic of Escherichia coli causing primary pyelonephritis. Lancet 2:1366–1369
    [Google Scholar]
  35. Vosti K L, Goldberg L M, Monto A S, Rantz L A. 1964; Host parasite interaction in patients with infections due to Escherichia coli. I. The serogrouping of E. coli from intestinal and extraintestinal sources. Journal of Clinical Investigation 43:2377–2385
    [Google Scholar]
  36. Weinstein R, Young L S. 1978; Phagocytic resistance of Escherichia coli K - 1 isolates and relationship to virulence. Journal of Clinical Microbiology 8:748–755
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00221317-31-3-175
Loading
/content/journal/jmm/10.1099/00221317-31-3-175
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error