1887

Abstract

This paper reports a case of polymicrobial bacteraemia and a case of monomicrobial bacteraemia identified by 16S ribosomal RNA gene sequencing. In the first case, a gram-negative aerobic coccobacillus was isolated with and from the blood culture of a 32-year-old intravenous drug addict with left thoracic empyema. In the second case, a gram-negative aerobic coccobacillus was isolated from the blood culture of an 82-year-old woman with colitis and septicaemic shock. Both gram-negative coccobacilli grew on chocolate agar as colonies of 1 mm in diameter after incubation for 24 h at 37°C in air with CO 5%, but only to pinpoint sizes on blood agar under the same incubation conditions. Both strains were factor V-dependent, but not factor X-dependent. For the first isolate, the Vitek system (NHI) showed that it was 56% likely to be and 40% ; whereas the API system (NH) showed that it was 58% likely to be and 42% . For the second isolate, the Vitek system (NHI) showed that it was 95% likely to be VIII; whereas the API system (NH) showed that it was 58% likely to be and 42% . 16S rRNA gene sequencing showed that there were four base differences between isolate 1 and and two base differences between isolate 2 and , indicating that both isolates most closely resembled a strain of . Only two cases of bacteraemia were found in the English scientific literature, one in a case of infective endocarditis and the other in a case of pancreatic abscess. Including the present two cases, the overall mortality of bacteraemia was 50%.

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/content/journal/jmm/10.1099/0022-1317-51-8-635
2002-08-01
2020-01-23
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http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/0022-1317-51-8-635
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