Four hundred and twenty-seven isolates from 427 cases of infantile diarrhoea in Poland, belonging to serogroups O18, O26, O44, O86, O126 and O127 and 150 isolates from 52 healthy children were examined for selected virulence properties. The presence of the plasmid pAA, a plasmid encoding enterohaemolysin, the genes encoding intimin (), bundle-forming pili (), Shiga toxins I and II (, ) and cytotoxic necrotising factor types 1 and 2 (, ) was investigated by PCR. Adhesion to HEp-2 cell monolayers was also tested and selected strains were investigated for the presence of P-fimbriae and haemolytic activity. Typical enteropathogenic isolates (typical EPEC, strains possessing and , but not ) were not found. The particular classes of among 427 isolates from ill children were: atypical EPEC ( , ), 21.3%; Shiga toxin-producing (STEC), 0.7%; diffusely adherent (DAEC), 4%; enteroaggregative (EAEC), 16.9%; necrotoxic type 1 (NTEC1), 0.2%; and cell-detaching (CDEC), 29%. With the exception of STEC, all the above classes of were found among the isolates from healthy children which comprised: atypical EPEC 8.0%, DAEC 6.7%, EAEC 17.3%, NTEC1 14.0% and CDEC 40.0%. Cell detachment (CD) was significantly associated with 3-h haemolytic activity. There was also strong correlation between haemolytic activity (Hly) and the presence of P-fimbriae. No correlation was found between the presence of the gene and CD, Hly or P-fimbriae.


Article metrics loading...

Loading full text...

Full text loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error