1887

Abstract

The complex presently comprises nine genomovars: (genomovar I), (genomovar II), genomovar III, (genomovar IV), (genomovar V), genomovar VI, (genomovar VII), (genomovar VIII) and (genomovar IX). Strains of each genomovar can colonise the respiratory tract of cystic fibrosis (CF) patients. However, the majority of infections in CF patients are caused by and genomovar III isolates. Accurate genomovar-level identification is best achieved through a polyphasic approach combining phenotypic and genotypic analyses. In the present study, the sensitivity and specificity of based genomovar specific primer pairs were evaluated with a collection of 508 complex isolates representing all nine genomovars. The assays for the identification of (sensitivity and specificity, 100%), genomovar III (sensitivity, 92%; specificity, 100%), and (sensitivity and specificity, 100%) were the most efficient. However, the genomovar I assay lacked sensitivity (72%) and cross-reacted with all isolates examined. Several new RFLP types were also revealed within the complex. One of these profiles was shared by a clinical and an environmental like isolate and by the type strain. The latter organism is a recently described soil bacterium. Its relationship to the various complex genomovars needs further study.

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2002-11-01
2019-11-23
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