1887

Abstract

has been reported as the cause of up to 15% of cases of antibiotic-associated diarrhoea (AAD) and may be diagnosed by detection of enterotoxin (CPEnt) in faeces. The performance of a commercial ELISA method for CPEnt, with culture and PCR methods to confirm the presence of enterotoxigenic , was evaluated in 200 consecutive specimens from patients with clinical details suggestive of AAD: 8% of the specimens were positive for CPEnt, 16% were positive for cytotoxin and 2% gave positive test results for both and toxins. Culture and PCR results confirmed the majority of ELISA results, although 2 (12.5%) reactive specimens were only weakly positive. is a potentially important cause of infective AAD and can be detected with the enterotoxin ELISA kit, although weak positive results should be considered with caution.

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2002-10-01
2021-10-23
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References

  1. Borriello SP, Larson HE, Welch AR, Barclay F, Stringer MF, Bartholomew BA. Enterotoxigenic Clostridium perfringens : a possible cause of antibiotic-associated diarrhoea. Lancet 1984; i:305–307
    [Google Scholar]
  2. Hancock P. Antibiotic associated diarrhoea: Clostridium difficile or C. perfringens ? Rev Med Microbiol ; 1997; 8 Suppl 1S66–S67 [CrossRef]
  3. Ransome NJ, Fitzgerald P, Wilcox MH. Antibiotic associated diarrhoea and Clostridium perfringens enterotoxin. 38th Interscience Conference on Antimicrobial Agents and Chemotherapy San Diego: 1998 Abstract K126
    [Google Scholar]
  4. Modi N, Wilcox MH. Evidence for antibiotic induced Clostridium perfringens diarrhoea. J Clin Pathol 2001; 54:748–751 [CrossRef]
    [Google Scholar]
  5. Czeczulin JR, Hanna PC, McClane BA. Cloning, nucleotide sequencing, and expression of the Clostridium perfringens enterotoxin gene in Escherichia coli . Infect Immun 1993; 61:3429–3439
    [Google Scholar]
  6. Czeczulin JR, Collie RE, McClane BA. Regulated expression of Clostridium perfringens enterotoxin in naturally cpe-negative type A, B, and C isolates of C. perfringens . Infect Immun 1996; 64:3301–3309
    [Google Scholar]
  7. Kokai-Kun JF, Songer JG, Czeczulin JR, Chen F, McClane BA. Comparison of Western immunoblots and gene detection assays for identification of potentially enterotoxigenic isolates of Clostridium perfringens . J Clin Microbiol 1994; 32:2533–2539
    [Google Scholar]
  8. Van Damme-Jongsten M, Wernars K, Notermans S. Cloning and sequencing of the Clostridium perfringens enterotoxin gene. Antonie Van Leeuwenhoek 1989; 56:181–190 [CrossRef]
    [Google Scholar]
  9. Van Damme-Jongsten M, Rodhouse J, Gilbert RJ, Notermans S. Synthetic DNA probes for detection of enterotoxigenic Clostridium perfringens strains isolated from outbreaks of food poisoning. J Clin Microbiol 1990; 28:131–133
    [Google Scholar]
  10. McClane BA, Strouse RJ. Rapid detection of Clostridium perfringens type A enterotoxin by enzyme-linked immunosorbent assay. J Clin Microbiol 1984; 19:112–115
    [Google Scholar]
  11. Bartholomew BA, Stringer MF, Watson GN, Gilbert RJ. Development and application of an enzyme linked immunosorbent assay for Clostridium perfringens type A enterotoxin. J Clin Pathol 1985; 38:222–228 [CrossRef]
    [Google Scholar]
  12. Giugliano LG, Stringer MF, Drasar BS. Detection of Clostridium perfringens enterotoxin by tissue culture and double-gel diffusion methods. J Med Microbiol 1983; 16:233–237 [CrossRef]
    [Google Scholar]
  13. Larson HE, Borriello SP. Infectious diarrhea due to Clostridium perfringens . J Infect Dis 1988; 157:390–391 [CrossRef]
    [Google Scholar]
  14. Daube G, China B, Simon P, Hvala K, Mainil J. Typing of Clostridium perfringens by in vitro amplification of toxin genes. J Appl Bacteriol 1994; 77:650–655 [CrossRef]
    [Google Scholar]
  15. Wilcox MH. Infective antibiotic-associated diarrhoea. In Wilcox MH. ed Infection highlights 1999−2000 Oxford, Health Press 200057–64
    [Google Scholar]
  16. Birkhead G, Vogt RL, Heun EM, Snyder JT, McClane BA. Characterization of an outbreak of Clostridium perfringens food poisoning by quantitative fecal culture and fecal enterotoxin measurement. J Clin Microbiol 1988; 26:471–474
    [Google Scholar]
  17. Berry PR, Rodhouse JC, Hughes S, Bartholomew BA, Gilbert RJ. Laboratory techniques.Evaluation of ELISA, RPLA, and Vero cell assays for detecting Clostridium perfringens enterotoxin in faecal specimens. J Clin Pathol 1988; 41:458–461 [CrossRef]
    [Google Scholar]
  18. Borriello SP, Barclay FE, Welch AR. et al. Epidemiology of diarrhoea caused by enterotoxigenic Clostridium perfringens . J Med Microbiol 1985; 20:363–372 [CrossRef]
    [Google Scholar]
  19. Wood M. When stool cultures from adult inpatients are appropriate. Lancet 2001; 357:901–902 [CrossRef]
    [Google Scholar]
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