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Abstract

picorna-like virus (EoPV) is an insect RNA virus that causes a lethal granulosis infection of larvae of the tea looper (). An internal ribosome entry site (IRES) mediates translation initiation of EoPV RNA. Here, bicistronic constructs were used to examine the 5′ untranslated region (UTR) of EoPV for IRES activity. The capacities of the EoPV 5′ UTR IRES and another insect virus IRES, the cricket paralysis virus intergenic region IRES, to mediate internal translation initiation in a variety of translation systems were also compared. The results demonstrated that the EoPV IRES functioned efficiently not only in mammalian cell-derived systems, but also in an insect cell-derived translation system. However, it functioned inefficiently in a plant cell-derived translation system. This study reveals the host preferences of the EoPV IRES and important differences in IRES function between the EoPV IRES and other characterized picorna-like insect viral IRESs.

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2007-10-01
2019-11-13
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vol. , part 10, pp. 2834 – 2838

Primer sets used for PCR amplification of a hemi-nested set of cDNA fragments corresponding to regions of the EoPV 5′ UTR [ PDF] (92 KB)



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