RT Journal Article SR Electronic(1) A1 Lu, Jie A1 Hu, Yuanyang A1 Hu, Liu A1 Zong, Shan A1 Cai, Dawei A1 Wang, Junping A1 Yu, Haiyang A1 Zhang, JiaminYR 2007 T1 Ectropis obliqua picorna-like virus IRES-driven internal initiation of translation in cell systems derived from different origins JF Journal of General Virology, VO 88 IS 10 SP 2834 OP 2838 DO https://doi.org/10.1099/vir.0.83201-0 PB Microbiology Society, SN 1465-2099, AB Ectropis obliqua picorna-like virus (EoPV) is an insect RNA virus that causes a lethal granulosis infection of larvae of the tea looper (Ectropis obliqua). An internal ribosome entry site (IRES) mediates translation initiation of EoPV RNA. Here, bicistronic constructs were used to examine the 5′ untranslated region (UTR) of EoPV for IRES activity. The capacities of the EoPV 5′ UTR IRES and another insect virus IRES, the cricket paralysis virus intergenic region IRES, to mediate internal translation initiation in a variety of translation systems were also compared. The results demonstrated that the EoPV IRES functioned efficiently not only in mammalian cell-derived systems, but also in an insect cell-derived translation system. However, it functioned inefficiently in a plant cell-derived translation system. This study reveals the host preferences of the EoPV IRES and important differences in IRES function between the EoPV IRES and other characterized picorna-like insect viral IRESs., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.83201-0