1887

Abstract

A novel virus, virus (BrBV), has been identified in the cabbage aphid using a method based on the random amplification of encapsidated RNA. The complete sequence of the RNA genome of BrBV has been determined. The positive-strand genomic RNA is 10 161 nt, excluding the 3′ poly(A) tail, and contains a single open reading frame (positions 793–9744) encoding a putative polyprotein of 2983 aa. The N-terminal part of the polyprotein shows similarity with the structural proteins of iflaviruses. The C-terminal part possesses consensus sequences of the helicase, cysteine protease and RNA-dependent RNA polymerase similar to those of iflaviruses and other picorna-like viruses. The highest sequence similarity observed was with iflaviruses from honeybee and an endoparasitic wasp. Replication and transmission of BrBV was not dependent on endoparasitic wasp infestation of the aphids.

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2007-09-01
2019-12-06
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References

  1. Allander, T., Tammi, M. T., Eriksson, M., Bjerkner, A., Tiveljung-Lindell, A. & Andersson, B. ( 2005; ). Cloning of a human parvovirus by molecular screening of respiratory tract samples. Proc Natl Acad Sci U S A 102, 12891–12896.[CrossRef]
    [Google Scholar]
  2. Altschul, S. F., Gish, W., Miller, W., Meyers, E. W. & Lipman, D. J. ( 1990; ). Basic local alignment search tool. J Mol Biol 215, 403–410.[CrossRef]
    [Google Scholar]
  3. Christian, P., Carstens, E., Domier, L., Johnson, J., Johnson, K., Nakashima, N., Scotti, P. & van der Wilk, F. ( 2005; ). Ilfavirus. In Virus Taxonomy: Eighth Report of the International Committee on Taxonomy of Viruses, pp. 779–782. Edited by C. M. Fauquet, M. A. Mayo, J. Maniloff, U. Desselberger & L. A. Ball. London: Elsevier/Academic Press.
  4. Felsenstein, J. ( 1989; ). phylip – Phylogeny inference package (version 3.2). Cladistics 5, 164–166.
    [Google Scholar]
  5. Fujiyuki, T., Takeuchi, H., Ono, M., Ohka, S., Sasaki, T., Nomoto, A. & Kubo, T. ( 2004; ). Novel insect picorna-like virus identified in the brains of aggressive worker honeybees. J Virol 78, 1093–1100.[CrossRef]
    [Google Scholar]
  6. Ghedin, E., Sengamalay, N. A., Shumway, M., Zaborsky, J., Feldblyum, T., Subbu, V., Spiro, D. J., Sitz, J., Koo, H. & other authors ( 2005; ). Large-scale sequencing of human influenza reveals the dynamic nature of viral genome evolution. Nature 437, 1162–1166.[CrossRef]
    [Google Scholar]
  7. Ghosh, R. C., Ball, B. V., Willcocks, M. M. & Carter, M. J. ( 1999; ). The nucleotide sequence of sacbrood virus of the honey bee: an insect picorna-like virus. J Gen Virol 80, 1541–1549.
    [Google Scholar]
  8. Koonin, E. V. & Dolja, V. V. ( 1993; ). Evolution and taxonomy of positive strand RNA viruses: implications of comparative analysis of amino acid sequences. Crit Rev Biochem Mol Biol 28, 375–430.[CrossRef]
    [Google Scholar]
  9. Lanzi, G., de Miranda, J. R., Boniotti, M. B., Cameron, C. E., Lavazza, A., Capucci, L., Camazine, S. M. & Cesare Rossi, C. ( 2006; ). Molecular and biological characterization of deformed wing virus of honeybees (Apis mellifera L.). J Virol 80, 4998–5009.[CrossRef]
    [Google Scholar]
  10. Moon, J. S., Domier, L. L., McCoppin, N. K., D'Arcy, C. J. & Jin, H. ( 1998; ). Nucleotide sequence analysis shows that Rhopalosiphum padi virus is a member of a novel group of insect-infecting RNA viruses. Virology 243, 54–65.[CrossRef]
    [Google Scholar]
  11. Ongus, J. R., Peters, D., Bonmatin, J.-M., Bengsch, E., Vlak, J. M. & van Oers, M. M. ( 2004; ). Complete sequence of a picorna-like virus of the genus Iflavirus replicating in the mite Varroa destructor. J Gen Virol 85, 3747–3755.[CrossRef]
    [Google Scholar]
  12. Ongus, J. R., Roode, E. C., Pleij, C. W. A., Vlak, J. M. & van Oers, M. M. ( 2006; ). The 5′ non-translated region of Varroa destructor virus 1 (genus Iflavirus): structure prediction and IRES activity in Lymantria dispar cells. J Gen Virol 87, 3397–3407.[CrossRef]
    [Google Scholar]
  13. Reineke, A. & Asgari, S. ( 2005; ). Presence of a novel small RNA-containing virus in a laboratory culture of the endoparasitic wasp Venturia canescens (Hymenoptera: Ichneumonidae). J Insect Physiol 51, 127–135.[CrossRef]
    [Google Scholar]
  14. Renault, S., Stasiak, K., Federici, B. & Bigot, Y. ( 2005; ). Commensal and mutualistic relationships of reoviruses with their parasitoid wasp hosts. J Insect Physiol 51, 137–148.[CrossRef]
    [Google Scholar]
  15. Sanchis, A., Latorre, A., Gonzalez-Candelas, F. & Michelina, J. M. ( 2000; ). An 18S rDNA-based molecular phylogeny of Aphidiinae (Hymenoptera: Brachonidae). Mol Phylogenet Evol 14, 180–194.[CrossRef]
    [Google Scholar]
  16. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  17. van Der Wilk, F., Dullemans, A. M., Verbeek, M. & van Den Heuvel, J. F. ( 1997; ). Nucleotide sequence and genomic organization of Acyrthosiphon pisum virus. Virology 238, 353–362.[CrossRef]
    [Google Scholar]
  18. van Munster, M., Dullemans, A. M., Verbeek, M., van Den Heuvel, J. F., Clerivet, A. & van Der Wilk, F. ( 2002; ). Sequence analysis and genomic organization of Aphid lethal paralysis virus: a new member of the family Dicistroviridae. J Gen Virol 83, 3131–3138.
    [Google Scholar]
  19. van Munster, M., Dullemans, A. M., Verbeek, M., van den Heuvel, J. F., Reinbold, C., Brault, V., Clerivet, A. & van der Wilk, F. ( 2003; ). A new virus infecting Myzus persicae has a genome organization similar to the species of the genus Densovirus. J Gen Virol 84, 165–172.[CrossRef]
    [Google Scholar]
  20. Wang, X., Zhang, J., Lu, J., Yi, F., Liu, C. & Hu, Y. ( 2004; ). Sequence analysis and genomic organization of a new insect picorna-like virus, Ectropis obliqua picorna-like virus, isolated from Ectropis obliqua. J Gen Virol 85, 1145–1151.[CrossRef]
    [Google Scholar]
  21. Witwer, C., Rauscher, S., Hofacker, I. L. & Stadler, P. F. ( 2001; ). Conserved RNA secondary structure elements in Picornaviridae genomes. Nucleic Acids Res 29, 5079–5089.[CrossRef]
    [Google Scholar]
  22. Wu, C.-Y., Lo, C.-F., Huang, C.-J., Yu, H.-T. & Wang, C.-H. ( 2002; ). The complete genome sequence of Perina nuda picorna-like virus, an insect-infecting RNA virus with a genome organization similar to that of the mammalian picornaviruses. Virology 294, 312–323.[CrossRef]
    [Google Scholar]
  23. Yang, X. & Cox-Foster, D. L. ( 2005; ). Impact of an ectoparasite on the immunity and pathology of an invertebrate: evidence for host immunosuppression and viral amplification. Proc Natl Acad Sci U S A 102, 7470–7475.[CrossRef]
    [Google Scholar]
  24. Yue, C. & Genersch, E. ( 2005; ). RT-PCR analysis of deformed wing virus in honeybees (Apis mellifera) and mites (Varroa destructor). J Gen Virol 86, 3419–3424.[CrossRef]
    [Google Scholar]
  25. Zuker, M. ( 2003; ). Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res 31, 3406–3415.[CrossRef]
    [Google Scholar]
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