1887

Abstract

The nucleotide sequence of the ascovirus (HvAV-3e) DNA genome was determined and characterized in this study. The circular genome consists of 186 262 bp, has a G+C content of 45.8 mol% and encodes 180 potential open reading frames (ORFs). Five unique homologous regions (s), 23 ‘baculovirus repeat ORFs' () and genes encoding a caspase homologue and several enzymes involved in nucleotide replication and metabolism were found in the genome. Several ascovirus (AV)-, iridovirus- and baculovirus-homologous genes were identified. The genome is significantly larger than the recently sequenced genomes of AV (TnAV-2c) and AV (SfAV-1a). Gene-parity plots and overall similarity of ORFs indicate that HvAV-3e is related more closely to SfAV-1a than to TnAV-2c.

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2007-04-01
2019-11-18
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References

  1. Ayres, M. D., Howard, S. C., Kuzio, J., Lopez-Ferber, M. & Possee, R. D. ( 1994; ). The complete DNA sequence of Autographa californica nuclear polyhedrosis virus. Virology 202, 586–605.[CrossRef]
    [Google Scholar]
  2. Bawden, A. L., Glassberg, K. J., Diggans, J., Shaw, R., Farmerie, W. & Moyer, R. W. ( 2000; ). Complete genomic sequence of the Amsacta moorei entomopoxvirus: analysis and comparison with other poxviruses. Virology 274, 120–139.[CrossRef]
    [Google Scholar]
  3. Bideshi, D. K., Renault, S., Stasiak, K., Federici, B. A. & Bigot, Y. ( 2003; ). Phylogenetic analysis and possible function of bro-like genes, a multigene family widespread among large double-stranded DNA viruses of invertebrates and bacteria. J Gen Virol 84, 2531–2544.[CrossRef]
    [Google Scholar]
  4. Bideshi, D. K., Tan, Y., Bigot, Y. & Federici, B. A. ( 2005; ). A viral caspase contributes to modified apoptosis for virus transmission. Genes Dev 19, 1416–1421.[CrossRef]
    [Google Scholar]
  5. Bideshi, D. K., Demattei, M. V., Rouleux-Bonnin, F., Stasiak, K., Tan, Y., Bigot, S., Bigot, Y. & Federici, B. A. ( 2006; ). Genomic sequence of the Spodoptera frugiperda ascovirus 1a, an enveloped, double-stranded DNA insect virus that manipulates apoptosis for viral reproduction. J Virol 80, 11791–11805.[CrossRef]
    [Google Scholar]
  6. Bigot, Y., Rabouille, A., Doury, G., Sizaret, P.-Y., Belbost, F., Hamelin, M.-H. & Periquet, G. ( 1997a; ). Biological and molecular features of the relationships between Diadromus pulchellus ascovirus, a parasitoid hymenopteran wasp (Diadromus pulchellus) and its lepidopteran host, Acrolepiopsis assectella. J Gen Virol 78, 1149–1163.
    [Google Scholar]
  7. Bigot, Y., Rabouille, A., Sizaret, P. Y., Hamelin, M. H. & Periquet, G. ( 1997b; ). Particle and genomic characteristics of a new member of the Ascoviridae: Diadromus pulchellus ascovirus. J Gen Virol 78, 1139–1147.
    [Google Scholar]
  8. Cheng, X.-W., Carner, G. R. & Brown, T. M. ( 1999; ). Circular configuration of the genome of ascoviruses. J Gen Virol 80, 1537–1540.
    [Google Scholar]
  9. Cheng, X.-W., Wang, L., Carner, G. R. & Arif, B. M. ( 2005; ). Characterization of three ascovirus isolates from cotton insects. J Invertebr Pathol 89, 193–202.[CrossRef]
    [Google Scholar]
  10. Coulombe, B. & Langelier, M. F. ( 2005; ). Functional dissection of the catalytic mechanism of mammalian RNA polymerase II. Biochem Cell Biol 83, 497–504.[CrossRef]
    [Google Scholar]
  11. Delaroque, N., Wolf, S., Muller, D. G. & Knippers, R. ( 2000; ). The brown algal virus EsV-1 particle contains a putative hybrid histidine kinase. Virology 273, 383–390.[CrossRef]
    [Google Scholar]
  12. Delcher, A. L., Harmon, D., Kasif, S., White, O. & Salzberg, S. L. ( 1999; ). Improved microbial gene identification with glimmer. Nucleic Acids Res 27, 4636–4641.[CrossRef]
    [Google Scholar]
  13. Espagne, E., Dupuy, C., Huguet, E., Cattolico, L., Provost, B., Martins, N., Poirie, M., Periquet, G. & Drezen, J.-M. ( 2004; ). Genome sequence of a polydnavirus: insights into symbiotic virus evolution. Science 306, 286–289.[CrossRef]
    [Google Scholar]
  14. Federici, B. A. ( 1983; ). Enveloped double-stranded DNA insect virus with novel structure and cytopathology. Proc Natl Acad Sci U S A 80, 7664–7668.[CrossRef]
    [Google Scholar]
  15. Federici, B. A. & Bigot, Y. ( 2003; ). Origin and evolution of polydnaviruses by symbiogenesis of insect DNA viruses in endoparasitic wasps. J Insect Physiol 49, 419–432.[CrossRef]
    [Google Scholar]
  16. Federici, B. A., Hamm, J. J. & Styer, E. L. ( 1991; ). Ascoviridae. In Atlas of Invertebrate Viruses, pp. 339–349. Edited by J. R. Adams & J. R. Bonami. Boca Raton, FL: CRC Press.
  17. Federici, B. A., Bigot, Y., Granados, R. R., Hamm, J. J., Miller, L. K., Newton, I., Stasiak, K. & Vlak, J. M. ( 2005; ). Family Ascoviridae. In Virus Taxonomy: Eighth Report of the International Committee on Taxonomy of Viruses, pp. 269–274. Edited by C. M. Fauquet, M. A. Mayo, J. Maniloff, U. Desselberger & L. A. Ball. San Diego: Elsevier Academic Press.
  18. Hu, Z. H., Arif, B. M., Jin, F., Martens, J. W. M., Chen, X. W., Sun, J. S., Zuidema, D., Goldbach, R. W. & Vlak, J. M. ( 1998; ). Distinct gene arrangement in the Buzura suppressaria single-nucleocapsid nucleopolyhedrovirus genome. J Gen Virol 79, 2841–2851.
    [Google Scholar]
  19. Jakob, N. J., Müller, K., Bahr, U. & Darai, G. ( 2001; ). Analysis of the first complete DNA sequence of an invertebrate iridovirus: coding strategy of the genome of Chilo iridescent virus. Virology 286, 182–196.[CrossRef]
    [Google Scholar]
  20. Kang, W., Suzuli, M., Evgueni, Z., Okano, K. & Maeda, S. ( 1999; ). Characterization of baculovirus repeated open reading frames (bro) in Bombyx mori nucleopolyhedrovirus. J Virol 73, 10339–10345.
    [Google Scholar]
  21. Li, L., Donly, C., Li, Q., Willis, L. G., Keddie, B. A., Erlandson, M. A. & Theilmann, D. A. ( 2002; ). Identification and genomic analysis of a second species of nucleopolyhedrovirus isolated from Mamestra configurata. Virology 297, 226–244.[CrossRef]
    [Google Scholar]
  22. Marchler-Bauer, A., Anderson, J. B., Cherukuri, P. F., DeWeese-Scott, C., Geer, L. Y., Gwadz, M., He, S., Hurwitz, D. I., Jackson, J. D. & other authors ( 2005; ). CDD: a Conserved Domain Database for protein classification. Nucleic Acids Res 33, D192–D196.[CrossRef]
    [Google Scholar]
  23. Miller, L. K. ( 1998; ). Ascoviruses. In The Insect Viruses, pp. 91–104. Edited by L. K. Miller & L. A. Ball. New York: Plenum Press.
  24. Newton, I. R. ( 2003; ). The biology and characterisation of the ascoviruses (Ascoviridae: Ascovirus) of Helicoverpa armigera Hübner and Helicoverpa punctigera Wallengren (Lepidoptera: Noctuidae) in Australia. Brisbane, Australia: University of Queensland.
  25. Parsons, J. D. ( 1995; ). Miropeats: graphical DNA sequence comparisons. Comput Appl Biosci 11, 615–619.
    [Google Scholar]
  26. Sambrook, J., Fritsch, E. F. & Maniatis, T. ( 1989; ). Molecular Cloning. A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  27. Stasiak, K., Renault, S., Demattei, M.-V., Bigot, Y. & Federici, B. A. ( 2003; ). Evidence for the evolution of ascoviruses from iridoviruses. J Gen Virol 84, 2999–3009.[CrossRef]
    [Google Scholar]
  28. Stasiak, K., Renault, S., Federici, B. A. & Bigot, Y. ( 2005; ). Characteristics of pathogenic and mutualistic relationships of ascoviruses in field populations of parasitoid wasps. J Insect Physiol 51, 103–115.[CrossRef]
    [Google Scholar]
  29. Wang, L., Xue, J., Seaborn, C. P., Arif, B. M. & Cheng, X.-W. ( 2006; ). Sequence and organization of the Trichoplusia ni ascovirus 2C (Ascoviridae) genome. Virology 354, 167–177.[CrossRef]
    [Google Scholar]
  30. Webb, B. A., Strand, M. R., Dickey, S. E., Beck, M. H., Hilgarth, R. S., Barney, W. E., Kadash, K., Kroemer, J. A., Lindstrom, K. G. & other authors ( 2006; ). Polydnavirus genomes reflect their dual roles as mutualists and pathogens. Virology 347, 160–174.[CrossRef]
    [Google Scholar]
  31. Whitfield, J. B. & Asgari, S. ( 2003; ). Virus or not? Phylogenetics of polydnaviruses and their wasp carriers. J Insect Physiol 49, 397–405.[CrossRef]
    [Google Scholar]
  32. Zemskov, E. A., Kang, W. & Maeda, S. ( 2000; ). Evidence for nucleic acid binding ability and nucleosome association of Bombyx mori nucleopolyhedrovirus BRO proteins. J Virol 74, 6784–6789.[CrossRef]
    [Google Scholar]
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