1887

Abstract

The function of cytotoxic T lymphocytes (CTLs) in rotavirus (RV) infection in humans is poorly understood. To date, no RV-specific human leukocyte antigen (HLA) class I-restricted T-cell epitopes have been described. In this study, four peptides derived from human RV Wa strain VP6 protein were predicted by computer algorithms and verified by an HLA*0201-binding assay. Two peptides with high affinity for HLA-A*0201 molecules were further assessed. The CTLs induced by P340–348 (TLLANVTAV)-loaded autologous dendritic cells from peripheral blood lymphocytes of HLA-A*0201-matched healthy donors released gamma interferon specifically upon stimulation with P340–348-loaded T2 cells. The CTLs lysed both P340–348-loaded T2 cells and human RV Wa strain-infected HLA-A*0201 Caco-2 cells in an antigen-specific and HLA-A*0201-restricted manner. At the same time, P340–348 was shown to be immunogenic in HLA-A*0201/Kb transgenic mice. It is proposed that P340–348 is an HLA-A*0201-restricted CTL epitope.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.82031-0
2006-11-01
2021-04-10
Loading full text...

Full text loading...

/deliver/fulltext/jgv/87/11/3393.html?itemId=/content/journal/jgv/10.1099/vir.0.82031-0&mimeType=html&fmt=ahah

References

  1. Chen A., Wang L., Zhang J., Zou L., Jia Z., Zhou W., Wan Y., Wu Y. 2005; H-2 Kd-restricted hepatitis B virus-derived epitope whose specific CD8+ T lymphocytes can produce gamma interferon without cytotoxicity. J Virol 79:5568–5576 [CrossRef]
    [Google Scholar]
  2. Franco M. A., Lefevre P., Willems P., Tosser G., Lintermanns P., Cohen J. 1994; Identification of cytotoxic T cell epitopes on the VP3 and VP6 rotavirus proteins. J Gen Virol 75:589–596 [CrossRef]
    [Google Scholar]
  3. Franco M. A., Tin C., Greenberg H. B. 1997; CD8+ T cells can mediate almost complete short-term and partial long-term immunity to rotavirus in mice. J Virol 71:4165–4180
    [Google Scholar]
  4. Jaimes M. C., Rojas O. L., González A. M. & 7 other authors 2002; Frequencies of virus-specific CD4+ and CD8+ T lymphocytes secreting gamma interferon after acute natural rotavirus infection in children and adults. J Virol 76:4741–4749 [CrossRef]
    [Google Scholar]
  5. McNeal M. M., Rae M. N., Ward R. L. 1997; Evidence that resolution of rotavirus infection in mice is due to both CD4 and CD8 cell-dependent activities. J Virol 71:8735–8742
    [Google Scholar]
  6. Offit P. A., Dudzik K. I. 1990; Rotavirus-specific cytotoxic T lymphocytes passively protect against gastroenteritis in suckling mice. J Virol 64:6325–6328
    [Google Scholar]
  7. Offit P. A., Clark H. F., Plotkin S. A. 1983; Response of mice to rotaviruses of bovine or primate origin assessed by radioimmunoassay, radioimmunoprecipitation, and plaque reduction neutralization. Infect Immun 42:293–300
    [Google Scholar]
  8. Offit P. A., Coupar B. E. H., Svoboda Y. M. & 7 other authors 1994; Induction of rotavirus-specific cytotoxic T lymphocytes by vaccinia virus recombinants expressing individual rotavirus genes. Virology 198:10–16 [CrossRef]
    [Google Scholar]
  9. Passoni L., Scardino A., Bertazzoli C., Gallo B., Coluccia A. M. L., Lemonnier F. A., Kosmatopoulos K., Gambacorti-Passerini C. 2002; ALK as a novel lymphoma-associated tumor antigen: identification of 2 HLA-A2.1-restricted CD8+ T-cell epitopes. Blood 99:2100–2106 [CrossRef]
    [Google Scholar]
  10. Player M. A., Barracchini K. C., Simonis T. B. & 7 other authors 1996; Differences in frequency distribution of HLA-A2 subtypes between North American and Italian white melanoma patients: relevance for epitope specific vaccination protocols. J Immunother Emphasis Tumor Immunol 19:357–363 [CrossRef]
    [Google Scholar]
  11. Rojas O. L., González A. M., González R., Pérez-Schael I., Greenberg H. B., Franco M. A., Angel J. 2003; Human rotavirus specific T cells: quantification by ELISPOT and expression of homing receptors on CD4+ T cells. Virology 314:671–679 [CrossRef]
    [Google Scholar]
  12. Sallusto F., Lanzavecchia A. 1994; Efficient presentation of soluble antigen by cultured human dendritic cells is maintained by granulocyte/macrophage colony-stimulating factor plus interleukin 4 and downregulated by tumor necrosis factor α . J Exp Med 179:1109–1118 [CrossRef]
    [Google Scholar]
  13. Shieh D. C., Lin D. T., Yang B. S., Kuan H. L., Kao K. J. 1996; High frequency of HLA-A*0207 subtype in Chinese population. Transfusion 36:818–821
    [Google Scholar]
  14. Tang B., Gilbert J. M., Matsui S. M., Greenberg H. B. 1997; Comparison of the rotavirus gene 6 from different species by sequence analysis and localization of subgroup-specific epitopes using site-directed mutagenesis. Virology 237:89–96 [CrossRef]
    [Google Scholar]
  15. Ward R. L. 2003; Possible mechanisms of protection elicited by candidate rotavirus vaccines as determined with the adult mouse model. Viral Immunol 16:17–24 [CrossRef]
    [Google Scholar]
  16. Wu Y., Zhang J., Chen S. & 8 other authors 2004; Frequencies of epitope-specific cytotoxic T lymphocytes in active chronic viral hepatitis B infection by using MHC class I peptide tetramers. Immunol Lett 92:253–258 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.82031-0
Loading
/content/journal/jgv/10.1099/vir.0.82031-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error