1887

Abstract

The existence of spliced mRNA in (CAV) was investigated, as three proteins appeared to be derived from a single 2.0 kb mRNA species. Human (TTV), which displays a number of genomic similarities to CAV, is known to transcribe three mRNA species, suggesting that CAV may also have multiple mRNAs. Northern analysis of infected chicken MDCC-MSB1 cells revealed a 2.0 kb mRNA 3 h post-infection (p.i.) and additional 1.6, 1.3 and 1.2 kb bands visible at 48 and 72 h p.i. MDCC-MSB1 or COS1 cells transfected with a CAV clone showed similar results. The poly(A) RNA of infected cells was subjected to RT-PCR using a suite of CAV-specific primers. The major 2.0 kb RNA reacted with every primer, but the 1.3 and 1.2 kb RNAs only annealed to certain primers. The 2.0 kb mRNA had no deletions or mutations and was capable of encoding all three known CAV proteins. The 1.3 kb RNA had a splice site joining nt 1222 to nt 1814 and encoded head/tail viral protein 1 (VP1) without a frameshift. In addition, the 1.2 kb RNA possessed a splice site joining nt 994 to nt 1095 and encoded several putative, novel proteins with frameshift mutations. These splice sites conformed to the previously described GT–AG splicing rule. One further 0.8 kb RNA species appeared to be derived from a homologous recombination event. Discovery of the presence of spliced mRNA in CAV strengthens the similarity between CAV and TTV.

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2006-08-01
2024-05-14
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References

  1. Aebi M., Hornig H., Padgett R. A., Reiser J., Weissmann C. 1986; Sequence requirements for splicing of higher eukaryotic nuclear pre-mRNA. Cell 47:555–565 [CrossRef]
    [Google Scholar]
  2. Akiyama Y., Kato S. 1974; Two cell lines from lymphomas of Marek's disease. Biken J 17:105–116
    [Google Scholar]
  3. Biagini P., Todd D., Bendinelli M. & 8 other authors 2004; Anellovirus. In Virus Taxonomy: Eighth Report 455 of the International Committee on Taxonomy of Viruses pp  335–341 Edited by Fauquet C. M., Mayo M. A., Maniloff J., Desselberger U., Ball L. A. London: Elsevier/Academic Press;
    [Google Scholar]
  4. Chen C., Okayama H. 1987; High-efficiency transformation of mammalian cells by plasmid DNA. Mol Cell Biol 7:2745–2752
    [Google Scholar]
  5. Chomczynski P., Sacchi N. 1987; Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156–159
    [Google Scholar]
  6. Hino S. 2002; TTV, a new human virus with single stranded circular DNA genome. Rev Med Virol 12:151–158 [CrossRef]
    [Google Scholar]
  7. Imai K., Yuasa N. 1990; Development of a microtest method for serological and virological examinations of chicken anemia agent. Nippon Juigaku Zasshi 52:873–875 [CrossRef]
    [Google Scholar]
  8. Islam M. R., Johne R., Raue R., Todd D., Muller H. 2002; Sequence analysis of the full-length cloned DNA of a chicken anaemia virus (CAV) strain from Bangladesh: evidence for genetic grouping of CAV strains based on the deduced VP1 amino acid sequences. J Vet Med B Infect Dis Vet Public Health 49:332–337 [CrossRef]
    [Google Scholar]
  9. Jones M. S., Kapoor A., Lukashov V. V., Simmonds P., Hecht F., Delwart E. 2005; New DNA viruses identified in patients with acute viral infection syndrome. J Virol 79:8230–8236 [CrossRef]
    [Google Scholar]
  10. Kamada K., Kamahora T., Kabat P., Hino S. 2004; Transcriptional regulation of TT virus: promoter and enhancer regions in the 1.2-kb noncoding region. Virology 321:341–348 [CrossRef]
    [Google Scholar]
  11. Kamahora T., Hino S., Miyata H. 2000; Three spliced mRNAs of TT virus transcribed from a plasmid containing the entire genome in COS1 cells. J Virol 74:9980–9986 [CrossRef]
    [Google Scholar]
  12. Miyata H., Tsunoda H., Kazi A., Yamada A., Khan M. A., Murakami J., Kamahora T., Shiraki K., Hino S. 1999; Identification of a novel GC-rich 113-nucleotide region to complete the circular, single-stranded DNA genome of TT virus, the first human circovirus. J Virol 73:3582–3586
    [Google Scholar]
  13. Niagro F. D., Forsthoefel A. N., Lawther R. P., Kamalanathan L., Ritchie B. W., Latimer K. S., Lukert P. D. 1998; Beak and feather disease virus and porcine circovirus genomes: intermediates between the geminiviruses and plant circoviruses. Arch Virol 143:1723–1744 [CrossRef]
    [Google Scholar]
  14. Nishizawa T., Okamoto H., Konishi K., Yoshizawa H., Miyakawa Y., Mayumi M. 1997; A novel DNA virus (TTV) associated with elevated transaminase levels in posttransfusion hepatitis of unknown etiology. Biochem Biophys Res Commun 241:92–97 [CrossRef]
    [Google Scholar]
  15. Noteborn M. H., de Boer G. F., van Roozelaar D. J. & 7 other authors 1991; Characterization of cloned chicken anemia virus DNA that contains all elements for the infectious replication cycle. J Virol 65:3131–3139
    [Google Scholar]
  16. Noteborn M. H., Kranenburg O., Zantema A., Koch G., de Boer G. F., van der Eb A. J. 1992; Transcription of the chicken anemia virus (CAV) genome and synthesis of its 52-kDa protein. Gene 118:267–271 [CrossRef]
    [Google Scholar]
  17. Noteborn M. H., Todd D., Verschueren C. A. & 7 other authors 1994; A single chicken anemia virus protein induces apoptosis. J Virol 68:346–351
    [Google Scholar]
  18. Okamoto H., Nishizawa T., Kato N., Ukita M., Ikeda H., Iizuka H., Miyakawa Y., Mayumi M. 1998; Molecular cloning and characterization of a novel DNA virus (TTV) associated with posttransfusion hepatitis of unknown etiology. Hepatol Res 10:1–16 [CrossRef]
    [Google Scholar]
  19. Okamoto H., Takahashi M., Kato N., Fukuda M., Tawara A., Fukuda S., Tanaka T., Miyakawa Y., Mayumi M. 2000; Sequestration of TT virus of restricted genotypes in peripheral blood mononuclear cells. J Virol 74:10236–10239 [CrossRef]
    [Google Scholar]
  20. Phenix K. V., Meehan B. M., Todd D., McNulty M. S. 1994; Transcriptional analysis and genome expression of chicken anaemia virus. J Gen Virol 75:905–909 [CrossRef]
    [Google Scholar]
  21. Qiu J., Kakkola L., Cheng F., Ye C., Soderlund-Venermo M., Hedman K., Pintel D. J. 2005; Human circovirus TT virus genotype 6 expresses six proteins following transfection of a full-length clone. J Virol 79:6505–6510 [CrossRef]
    [Google Scholar]
  22. Sambrook J., Russell D. W. 2001 Molecular Cloning: a Laboratory Manual , 3rd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  23. Todd D., Creelan J. L., Mackie D. P., Rixon F., McNulty M. S. 1990; Purification and biochemical characterization of chicken anaemia agent. J Gen Virol 71:819–823 [CrossRef]
    [Google Scholar]
  24. Yamaguchi S., Imada T., Kaji N., Mase M., Tsukamoto K., Tanimura N., Yuasa N. 2001; Identification of a genetic determinant of pathogenicity in chicken anaemia virus. J Gen Virol 82:1233–1238
    [Google Scholar]
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