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Abstract

A characterization of the gene from (VACV) strain Western Reserve (WR) is presented. Computational analyses of the gene indicated high conservation in orthopoxviruses but no orthologues outside the . To characterize the B14 protein, the gene was expressed in and recombinant protein was purified and used to generate a rabbit polyclonal antiserum. This antiserum recognized a 15 kDa cytoplasmic protein in mammalian cells that were transfected with the gene or infected with VACV WR, but not from cells infected with a VACV mutant (vΔB14) from which the gene was deleted. Compared to wild-type and revertant virus controls, vΔB14 had normal growth kinetics in cell culture. The virulence of vΔB14 was assessed in two models. Mice infected intranasally with vΔB14 had similar weight loss compared to the controls, but in C57BL/6 mice infected intradermally vΔB14 induced a smaller lesion size compared with controls. Moreover, intradermal infection with vΔB14 caused an increased infiltration of cells into the infected lesion despite the smaller lesion size. Therefore, B14 is an intracellular protein that is non-essential for virus replication in cell culture but contributes to virus virulence and affects the host response to infection.

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2006-06-01
2019-10-21
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vol. , part 6, pp. 1451 - 1458

Analysis of recombinant VACV genomes by PCR

Characterization of the VACV genomes

Growth kinetics of vΔB14

B14 protein did not affect virus virulence in a murine i.n. model under the conditions tested [Single PDF file](154 KB)



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