1887

Abstract

Monolayers of Hep G2/C3A cells were inoculated with genotype 1 (HEV) mixed with either anti-HEV or an appropriate control. After 5 or 6 days, cell monolayers were stained with anti-HEV and infected cells were identified by immunofluorescence microscopy and counted. Anti-HEV from vaccinated or infected rhesus monkeys neutralized the virus, as did mAbs that recognized epitopes on the C terminus of a recombinant vaccine protein. Antibodies were broadly cross-reactive, since convalescent serum from animals infected with any one of the four mammalian genotypes all neutralized the genotype 1 virus.

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2006-03-01
2021-07-30
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References

  1. Emerson S. U., Purcell R. H. 2003; Hepatitis E virus. Rev Med Virol 13:145–154 [CrossRef]
    [Google Scholar]
  2. Emerson S. U., Anderson D., Arankalle A., Meng X.-J., Purdy M., Schlauder G. G., Tsarev S. A. 2004; Hepevirus . In Virus Taxonomy: Eighth Report of the International Committee on Taxonomy of Viruses pp  851–855 Edited by Fauquet C. M., Mayo M. A., Maniloff J., Desselberger U., Ball L. A. London: Elsevier;
    [Google Scholar]
  3. Emerson S. U., Arankalle V. A., Purcell R. H. 2005; Thermal stability of hepatitis E virus. J Infect Dis 192:930–933 [CrossRef]
    [Google Scholar]
  4. Engle R. E., Yu C., Emerson S. U., Meng X.-J., Purcell R. H. 2002; Hepatitis E virus (HEV) capsid antigens derived from viruses of human and swine origin are equally efficient for detecting anti-HEV by enzyme immunoassay. J Clin Microbiol 40:4576–4580 [CrossRef]
    [Google Scholar]
  5. Mast E. E., Alter M. J., Holland P. V., Purcell R. H. 1998; Evaluation of assays for antibody to hepatitis E virus by a serum panel. Hepatology 27:857–861 [CrossRef]
    [Google Scholar]
  6. Meng X. J. 2000; Novel strains of hepatitis E virus identified from humans and other animal species: is hepatitis E a zoonosis?. J Hepatol 33:842–845 [CrossRef]
    [Google Scholar]
  7. Meng J., Pillot J., Dai X., Fields H. A., Khudyakov Y. E. 1998; Neutralization of different geographic strains of the hepatitis E virus with anti-hepatitis E virus-positive serum samples obtained from different sources. Virology 249:316–324 [CrossRef]
    [Google Scholar]
  8. National Research Council. 1996 Guide for the Care and Use of Laboratory Animals Washington, DC: National Academy Press;
    [Google Scholar]
  9. Purcell R. H., Emerson S. U. 2001; Hepatitis E virus. In Fields Virology , 4th edn. pp  3051–3061 Edited by Knipe D., Howley P., Griffin D., Lamb R., Martin M., Roizman B., Straus S. Philadelphia: Lippincott, Williams & Wilkins;
    [Google Scholar]
  10. Purcell R. H., Emerson S. U. 2005; Hepatitis E virus. In Principles and Practice of Infectious Diseases , 6th edn. pp  2204–2217 Edited by Mandell G. L., Bennett J. E., Dolin R. Philadelphia: Elsevier/Churchill Livingstone;
    [Google Scholar]
  11. Purcell R. H., Nguyen H., Shapiro M., Engle R. E., Govindarajan S., Blackwelder W. C., Wong D. C., Prieels J.-P., Emerson S. U. 2003; Pre-clinical immunogenicity and efficacy trial of a recombinant hepatitis E vaccine. Vaccine 21:2607–2615 [CrossRef]
    [Google Scholar]
  12. Reyes G. R., Purdy M. A., Kim J. P., Luk K. C., Young L. M., Fry K. E., Bradley D. W. 1990; Isolation of a cDNA from the virus responsible for enterically transmitted non-A, non-B hepatitis. Science 247:1335–1339 [CrossRef]
    [Google Scholar]
  13. Robinson R. A., Burgess W. H., Emerson S. U., Leibowitz R. S., Sosnovtesa S. A., Purcell R. H. 1998; Structural characterization of recombinant hepatitis E virus ORF2 proteins in baculovirus-infected insect cells. Protein Expr Purif 12:75–84 [CrossRef]
    [Google Scholar]
  14. Schofield D. J., Glamann J., Emerson S. U., Purcell R. H. 2000; Identification by phage display and characterization of two neutralizing chimpanzee monoclonal antibodies to the hepatitis E virus capsid protein. J Virol 74:5548–5555 [CrossRef]
    [Google Scholar]
  15. Schofield D. J., Purcell R. H., Nguyen H. T., Emerson S. U. 2003; Monoclonal antibodies that neutralize HEV recognize an antigenic site at the carboxyterminus of an ORF2 protein vaccine. Vaccine 22:257–267 [CrossRef]
    [Google Scholar]
  16. Takahashi K., Kitajima N., Abe N., Mishiro S. 2004; Complete or near-complete nucleotide sequences of hepatitis E virus genome recovered from a wild boar, a deer, and four patients who ate the deer. Virology 330:501–505 [CrossRef]
    [Google Scholar]
  17. Tam A. W., White R., Yarbough P. O., Murphy B. J., McAtee C. P., Lanford R. E., Fuerst T. R. 1997; In vitro infection and replication of hepatitis E virus in primary cynomolgus macaque hepatocytes. Virology 238:94–102 [CrossRef]
    [Google Scholar]
  18. Tei S., Kitajima N., Takahashi K., Mishiro S. 2003; Zoonotic transmission of hepatitis E virus from deer to human beings. Lancet 362:371–373 [CrossRef]
    [Google Scholar]
  19. Tsarev S. A., Tsareva T. S., Emerson S. U., Govindarajan S., Shapiro M., Gerin J. L., Purcell R. H. 1997; Recombinant vaccine against hepatitis E: dose response and protection against heterologous challenge. Vaccine 15:1834–1838 [CrossRef]
    [Google Scholar]
  20. Zhang M., Emerson S. U., Nguyen H., Engle R., Govindarajan S., Blackwelder W. C., Gerin J., Purcell R. H. 2002; Recombinant vaccine against hepatitis E: duration of protective immunity in rhesus macaques. Vaccine 20:3285–3291 [CrossRef]
    [Google Scholar]
  21. Zhou Y.-H., Purcell R. H., Emerson S. U. 2004; An ELISA for putative neutralizing antibodies to hepatitis E virus detects antibodies to genotypes 1, 2, 3, and 4. Vaccine 22:2578–2585 [CrossRef]
    [Google Scholar]
  22. Zhou Y. H., Purcell R. H., Emerson S. U. 2005; A truncated ORF2 protein contains the most immunogenic site on ORF2: antibody responses to non-vaccine sequences following challenge of vaccinated and non-vaccinated macaques with hepatitis E virus. Vaccine 23:3157–3165 [CrossRef]
    [Google Scholar]
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