1887

Abstract

Expression vectors were constructed from 35S promoter-containing full-length cDNA clones of Zygocactus virus X (ZVX). The expression of foreign genes was driven by the ZVX coat protein (cp) subgenomic promoter. It was successful only when the variable region downstream of the conserved putative promoter region GSTTAAGTT(X)GAA was retained. Most of the ZVX cp gene, except for a short 3′ part, was replaced by the corresponding sequence of the related Schlumbergera virus X (SVX) and its cp subgenomic promoter to enable encapsidation of the transcribed RNA by an SVX/ZVX hybrid cp. Vector-expressed cp of (BNYVV) assembled in , and leaves into particles resembling true BNYVV particles. The virus produced from these constructs retained its ability to express BNYVV cp in local infections during successive passages on . This ability was lost, however, in the rarely occurring systemic infections.

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2006-02-01
2019-11-18
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References

  1. Awram, P., Gardner, R. C., Forster, R. L. & Bellamy, A. R. ( 2002; ). The potential of plant viral vectors and transgenic plants for subunit vaccine production. Adv Virus Res 58, 81–124.
    [Google Scholar]
  2. Brigneti, G., Martín-Hernández, A. M., Jin, H., Chen, J., Baulcombe, D. C., Baker, B. & Jones, J. D. G. ( 2004; ). Virus-induced gene silencing in Solanum species. Plant J 39, 264–272.[CrossRef]
    [Google Scholar]
  3. Chen, J., Shi, Y.-H., Adams, M. J. & Chen, J.-P. ( 2005; ). The complete sequence of the genomic RNA of an isolate of Lily virus X (genus Potexvirus). Arch Virol 150, 825–832.[CrossRef]
    [Google Scholar]
  4. Culver, J. N. ( 1996; ). Tobamovirus cross protection using a potexvirus vector. Virology 226, 228–235.[CrossRef]
    [Google Scholar]
  5. Donson, J., Kearney, C. M., Hilf, M. E. & Dawson, W. O. ( 1991; ). Systemic expression of a bacterial gene by a tobacco mosaic virus-based vector. Proc Natl Acad Sci U S A 88, 7204–7208.[CrossRef]
    [Google Scholar]
  6. Flatken, S. & Maiss, E. ( 2005; ). Construction and agroinfection of a Potato virus M full-length clone. In Fourth Joint Meeting of Dutch and German Plant Virologists, Wageningen, 10–11 March 2005, p. 22.
  7. Gilardi, P., García-Luque, I. & Serra, M. T. ( 1998; ). Pepper mild mottle virus coat protein alone can elicit the Capsicum spp. L3 gene-mediated resistance. Mol Plant Microbe Interact 11, 1253–1257.[CrossRef]
    [Google Scholar]
  8. Gleba, Y., Marillonnet, S. & Klimyuk, V. ( 2004; ). Engineering viral expression vectors for plants: the ‘full virus' and the ‘deconstructed virus' strategies. Curr Opin Plant Biol 7, 182–188.[CrossRef]
    [Google Scholar]
  9. Haeberlé, A.-M., Stussi-Garaud, C., Schmitt, C., Garaud, J.-C., Richards, K. E., Guilley, H. & Jonard, G. ( 1994; ). Detection by immunogold labelling of P75 readthrough protein near an extremity of beet necrotic yellow vein virus particles. Arch Virol 134, 195–203.[CrossRef]
    [Google Scholar]
  10. Koenig, R. & Stein, B. ( 1990; ). Distribution of beet necrotic yellow vein virus in mechanically inoculated sugarbeet plantlets of cultivars with different degrees of rhizomania resistance. In Proceedings of the First Symposium of the International Working Group on Plant Viruses with Fungal Vectors, Braunschweig, Germany, 21–24 August 1990, pp. 87–90.
  11. Koenig, R., Pleij, C. W. A., Loss, S., Burgermeister, W., Aust, H. & Schiemann, J. ( 2004; ). Molecular characterisation of potexviruses isolated from three different genera in the family Cactaceae. Arch Virol 149, 903–914.[CrossRef]
    [Google Scholar]
  12. Liu, L. & Lomonossoff, G. P. ( 2002; ). Agroinfection as a rapid method for propagating Cowpea mosaic virus-based constructs. J Virol Methods 105, 343–348.[CrossRef]
    [Google Scholar]
  13. Lu, R., Martín-Hernández, A. M., Peart, J. R., Malcuit, I. & Baulcombe, D. C. ( 2003; ). Virus-induced gene silencing in plants. Methods 30, 296–303.[CrossRef]
    [Google Scholar]
  14. MacFarlane, S. A. & Popovich, A. H. ( 2000; ). Efficient expression of foreign proteins in roots from tobravirus vectors. Virology 267, 29–35.[CrossRef]
    [Google Scholar]
  15. Memelink, J., van der Vlugt, C. I. M., Linthorst, H. J. M., Derks, A. F. L. M., Asjes, C. J. & Bol, J. F. ( 1990; ). Homologies between the genomes of a carlavirus (lily symptomless virus) and a potexvirus (lily virus X) from lily plants. J Gen Virol 71, 917–924.[CrossRef]
    [Google Scholar]
  16. Milicic, D., Plese, N., Bercks, R., Brandes, J., Casper, R. & Chessin, M. ( 1966; ). Vergleichende serologische und elektronenmikroskopische Untersuchungen an Isolaten des Kakteen-X-Virus. Phytopathol Z 55, 211–217 (in German).[CrossRef]
    [Google Scholar]
  17. Milne, R. G. ( 1984; ). Electron microscopy for the identification of plant viruses in in vitro preparations. Methods Virol 7, 87–120.
    [Google Scholar]
  18. Milne, R. G. & Lesemann, D.-E. ( 1984; ). Immunosorbent electron microscopy in plant virus studies. Methods Virol 8, 85–101.
    [Google Scholar]
  19. Naylor, M., Reeves, J., Cooper, J. I., Edwards, M.-L. & Wang, H. ( 2005; ). Construction and properties of a gene-silencing vector based on Poplar mosaic virus (genus Carlavirus). J Virol Methods 124, 27–36.[CrossRef]
    [Google Scholar]
  20. Pogue, G. P., Lindbo, J. A., Garger, S. J. & Fitzmaurice, W. P. ( 2002; ). Making an ally from an enemy: plant virology and the new agriculture. Annu Rev Phytopathol 40, 45–74.[CrossRef]
    [Google Scholar]
  21. Porta, C. & Lomonossoff, G. P. ( 2002; ). Viruses as vectors for the expression of foreign sequences in plants. Biotechnol Genet Eng Rev 19, 245–291.[CrossRef]
    [Google Scholar]
  22. Schmitt, C., Balmori, E., Jonard, G., Richards, K. E. & Guilley, H. ( 1992; ). In vitro mutagenesis of biologically active transcripts of beet necrotic yellow vein virus RNA 2: evidence that a domain of the 75-kDa readthrough protein is important for efficient virus assembly. Proc Natl Acad Sci U S A 89, 5715–5719.[CrossRef]
    [Google Scholar]
  23. Shivprasad, S., Pogue, G. P., Lewandowski, D. J., Hidalgo, J., Donson, J., Grill, L. K. & Dawson, W. O. ( 1999; ). Heterologous sequences greatly affect foreign gene expression in tobacco mosaic virus-based vectors. Virology 255, 312–323.[CrossRef]
    [Google Scholar]
  24. Toth, R. L., Pogue, G. P. & Chapman, S. ( 2002; ). Improvement of the movement and host range properties of a plant virus vector through DNA shuffling. Plant J 30, 593–600.[CrossRef]
    [Google Scholar]
  25. Yamamiya, A. & Shirako, Y. ( 2000; ). Construction of full-length cDNA clones to Soil-borne wheat mosaic virus RNA1 and RNA2 from which infectious cDNAs are transcribed in vitro: virion formation and systemic infection without expression of the N-terminal and C-terminal extensions to the capsid protein. Virology 277, 66–75.[CrossRef]
    [Google Scholar]
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