1887

Abstract

An artificial open reading frame (ORF) for bovine alpha interferon (boIFN-) with the codon preference of (BHV-1) glycoprotein B was constructed to assess the effect of expression of boIFN- by BHV-1 from an expression cassette. Transient expression of the ORF revealed that transfected cells secreted substantial amounts of biologically active boIFN-, which moderately inhibited replication of BHV-1 after stimulation of bovine cells with 10 U ml. The boIFN--encoding expression cassette was recombined into the glycoprotein E locus of the glycoprotein E-negative BHV-1 vaccine strain GKD. Cells infected with the resulting recombinant BHV-1/boIFN- secreted up to 10 U boIFN- per ml cell culture supernatant, which is about 40- to more than 100-fold the activity reached with other virus expression systems. Bioassays demonstrated that the BHV-1-expressed interferon induced a rapid and sustained antiviral state in stimulated bovine cells. Analysis of the growth properties of the recombinant revealed, depending on the cell line used, no or only slight inhibition in direct spreading from cell to cell and a modest delay in virus egress from infected cells. Final titres, however, were comparable to those reached by the parent strain. Penetration into cells was not affected. The results from this study demonstrate that BHV-1/boIFN- expresses high levels of boIFN-, grows to high titres in cell culture and thus represents a potential alternative means to deliver endogenously produced boIFN- for a period of time.

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2005-10-01
2019-10-22
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