1887

Abstract

Infectious clone technology provides an opportunity to study the molecular basis of arthropod–virus interactions in detail. This study describes the development of an infectious clone of the prototype yellow fever virus Asibi strain (YFV-As) with the purpose of identifying sequences or domains that influence infection dynamics in the mosquito vector. The full-length cDNA of YFV-As virus was produced from RT-PCR products of parental viral RNA. These were cloned into a low-copy-number plasmid previously used to develop the YFV-17D infectious clone (pACNR/FLYF-17D). Virus recovered from the infectious clone exhibited biological characteristics similar to those of the parental YFV-As, including replication kinetics, reactivity to flavivirus cross-reactive and YFV-specific antibodies and infection and dissemination rates in , the principal mosquito vector of YFV. These data provide the basis for future studies with chimeric Asibi/17D viruses to identify the determinants of vaccine attenuation in the vector.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.80746-0
2005-06-01
2019-10-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/86/6/vir861747.html?itemId=/content/journal/jgv/10.1099/vir.0.80746-0&mimeType=html&fmt=ahah

References

  1. Barrett, A. D. T. & Monath, T. P. ( 2003; ). Epidemiology and ecology of yellow fever virus. Adv Virus Res 61, 291–315.
    [Google Scholar]
  2. Bredenbeek, P. J., Kooi, E. A., Lindenbach, B., Huijkman, N., Rice, C. M. & Spaan, W. J. M. ( 2003; ). A stable full-length yellow fever virus cDNA clone and the role of conserved RNA elements in flavivirus replication. J Gen Virol 84, 1261–1268.[CrossRef]
    [Google Scholar]
  3. Dunster, L. M., Wang, H., Ryman, K. D., Miller, B. R., Watowich, S. J., Minor, P. D. & Barrett, A. D. ( 1999; ). Molecular and biological changes associated with HeLa cell attenuation of wild-type yellow fever virus. Virology 261, 309–318.[CrossRef]
    [Google Scholar]
  4. Gould, E. A., Buckley, A. & Cammack, N. ( 1985; ). Use of a biotin-streptavidin interaction to improve flavivirus detection by immunofluorescence and ELISA tests. J Virol Methods 11, 41–48.[CrossRef]
    [Google Scholar]
  5. Gritsun, T. S. & Gould, E. A. ( 1998; ). Development and analysis of a tick-borne encephalitis virus infectious clone using a novel and rapid strategy. J Virol Methods 76, 109–120.[CrossRef]
    [Google Scholar]
  6. Hahn, C. H., Dalrymple, J. M., Strauss, J. H. & Rice, C. M. ( 1987; ). Comparison of the virulent Asibi strain of yellow fever virus with the 17D vaccine strain derived from it. Proc Natl Acad Sci U S A 84, 2019–2023.[CrossRef]
    [Google Scholar]
  7. Hayasaka, D., Gritsun, T. S., Yoshii, K. & 7 other authors ( 2004; ). Amino acid changes responsible for attenuation of virus neurovirulence in an infectious cDNA clone of the Oshima strain of tick-borne encephalitis virus. J Gen Virol 85, 1007–1018.[CrossRef]
    [Google Scholar]
  8. Higgs, S. ( 2004; ). How do mosquito vectors live with their viruses? In Microbe–Vector Interactions in Vector-Borne Diseases (Society for General Microbiology Symposium no. 63), pp. 103–137. Edited by S. H. Gillespie, G. L. Smith & A. Osbourn. Cambridge: Cambridge University Press.
  9. Higgs, S., Olson, K. E., Kamrud, K. I., Powers, A. M. & Beaty, B. J. ( 1997; ). Viral expression systems and viral infections in insects. In The Molecular Biology of Insect Disease Vectors: a Methods Manual, pp. 459–483. Edited by J. M. Crampton, C. B. Beard & C. Louis. London: Chapman & Hall.
  10. Jennings, A. D., Gibson, C. A., Miller, B. R. & 12 other authors ( 1994; ). Analysis of a yellow fever virus isolated from a fatal case of vaccine-associated human encephalitis. J Infect Dis 169, 512–518.[CrossRef]
    [Google Scholar]
  11. Kapoor, M., Zhang, L., Mohan, P. M. & Padmanabhan, R. ( 1995; ). Synthesis and characterization of an infectious dengue virus type-2 RNA genome (New Guinea C strain). Gene 162, 175–180.[CrossRef]
    [Google Scholar]
  12. Kinney, R. M., Butrapet, S., Chang, G.-J. J., Tsuchiya, K. R., Roehrig, J. T., Bhamarapravati, N. & Gubler, D. J. ( 1997; ). Construction of infectious cDNA clones for dengue 2 virus: strain 16681 and its attenuated vaccine derivative, strain PDK-53. Virology 230, 300–308.[CrossRef]
    [Google Scholar]
  13. Lai, C. B., Zhao, B. T., Hori, H. & Bray, M. ( 1991; ). Infectious RNA transcribed from stably cloned full-length cDNA of dengue type 4 virus. Proc Natl Acad Sci U S A 88, 5139–5143.[CrossRef]
    [Google Scholar]
  14. Lindenbach, B. D. & Rice, C. M. ( 2003; ). Molecular biology of flaviviruses. Adv Virus Res 59, 23–61.
    [Google Scholar]
  15. Mandl, C. W., Ecker, M., Holzmann, H., Kunz, C. & Heinz, F. X. ( 1997; ). Infectious cDNA clones of tick-borne encephalitis virus European subtype prototypic strain Nuedoerfl and high virulence strain Hypr. J Gen Virol 78, 1049–1057.
    [Google Scholar]
  16. McArthur, M. A., Suderman, M. T., Mutebi, J. P., Xiao, S. Y. & Barrett, A. D. T. ( 2003; ). Molecular characterization of a hamster viscerotropic strain of yellow fever virus. J Virol 77, 1462–1468.[CrossRef]
    [Google Scholar]
  17. Miller, B. R. & Adkins, D. ( 1988; ). Biological characterization of plaque-size variants of yellow fever virus in mosquitoes and mice. Acta Virol 32, 227–234.
    [Google Scholar]
  18. Miller, B. R. & Mitchell, C. J. ( 1991; ). Genetic selection of a flavivirus-refractory strain of the yellow fever mosquito Aedes aegypti. Am J Trop Med Hyg 45, 399–407.
    [Google Scholar]
  19. Mutebi, J. P. & Barrett, A. D. T. ( 2002; ). The epidemiology of yellow fever in Africa. Microbes Infect 4, 1459–1468.[CrossRef]
    [Google Scholar]
  20. Nickells, M. & Chambers, T. J. ( 2003; ). Neuroadapted yellow fever virus 17D: determinants in the envelope protein govern neuroinvasiveness for SCID mice. J Virol 77, 12232–12242.[CrossRef]
    [Google Scholar]
  21. Rice, C. M., Grakoui, A., Galler, R. & Chambers, T. J. ( 1989; ). Transcription of infectious yellow fever RNA from full-length cDNA templates produced by in vitro ligation. New Biol 1, 285–296.
    [Google Scholar]
  22. Robertson, S. E., Hull, B. P., Tomori, O., Bele, O., LeDuc, J. W. & Esteves, K. ( 1996; ). Yellow fever: a decade of reemergence. JAMA 276, 1157–1162.[CrossRef]
    [Google Scholar]
  23. Sambrook, J., Fritsch, E. & Maniatis, T. ( 1989; ). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  24. Shi, P. Y., Tilgner, M., Lo, M. K., Kent, K. A. & Bernard, K. A. ( 2002; ). Infectious cDNA clone of the epidemic West Nile virus from New York City. J Virol 76, 5847–5856.[CrossRef]
    [Google Scholar]
  25. Stokes, A., Bauer, J. H. & Hudson, N. P. ( 1928; ). The transmission of yellow fever to Macacus rhesus. JAMA 96, 253–254.
    [Google Scholar]
  26. Sumiyoshi, H., Hoke, C. H. & Trent, D. W. ( 1992; ). Infectious Japanese encephalitis virus RNA can be synthesized from in vitro-ligated cDNA templates. J Virol 66, 5425–5431.
    [Google Scholar]
  27. Theiler, M. ( 1951; ). The virus. In Yellow Fever, pp. 39–136. Edited by G. K. Strode. New York: McGraw-Hill.
  28. Theiler, M. & Smith, H. H. ( 1937; ). The effect of prolonged cultivation in vitro upon the pathogenicity of yellow fever virus. J Exp Med 65, 767–786.[CrossRef]
    [Google Scholar]
  29. Vanlandingham, D. L., Hong, C., Klingler, K., Tsetsarkin, K., McElroy, K. L., Powers, A. M., Lehane, M. J. & Higgs, S. ( 2005; ). Differential infectivities of o'nyong-nyong and chikungunya virus isolates in Anopheles gambiae and Aedes aegypti mosquitoes. Am J Trop Med Hyg 72 (in press).
    [Google Scholar]
  30. Whitman, L. ( 1939; ). Failure of Aedes aegypti to transmit yellow fever cultured virus (17D). Am J Trop Med Hyg 19, 19–26.
    [Google Scholar]
  31. Yamshchikov, V. F., Wengler, G., Perelygin, A. A., Brinton, M. A. & Compans, R. W. ( 2001; ). An infectious clone of the West Nile flavivirus. Virology 281, 294–304.[CrossRef]
    [Google Scholar]
  32. Yun, S. I., Kim, S. Y., Rice, C. M. & Lee, Y. M. ( 2003; ). Development and application of a reverse genetics system for Japanese encephalitis virus. J Virol 77, 6450–6465.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.80746-0
Loading
/content/journal/jgv/10.1099/vir.0.80746-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error