1887

Abstract

The alternative oxidase (AOX) is the terminal oxidase of the cyanide-resistant alternative respiratory pathway in plants and has been implicated in resistance to viruses. When tobacco mosaic virus (TMV) vectors were used to drive very high levels of expression of either AOX or AOX mutated in its active site (AOX-E), virus spread was enhanced. This was visualized as the induction of larger hypersensitive-response lesions after inoculation onto -genotype tobacco than those produced by vectors bearing sequences of comparable length [the green fluorescent protein () gene sequence or antisense ] or the ‘empty’ viral vector. Also, in the highly susceptible host , systemic movement of TMV vectors expressing AOX or AOX-E was faster than that of TMV constructs bearing or antisense sequences. Notably, in , TMV.AOX and TMV.AOX-E induced symptoms that were severe and ultimately included cell death, whereas the empty vector, TMV.GFP and the TMV vector expressing antisense sequences never induced necrosis. The results show that, if expressed at sufficiently high levels, active and inactive AOX proteins can affect virus spread and symptomology in plants.

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2004-12-01
2019-11-20
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