1887

Abstract

To understand better the pathogenesis of foot-and-mouth disease (FMD), the levels of viral RNA in various bovine tissues during the acute and persistent stages of FMD virus (FMDV) infection were investigated by using quantitative RT-PCR. The viral RNA levels in the tissues examined had peaked by day 1 post-infection (p.i.) and were markedly different among the tissues examined. The epithelium collected from sites of lesion development, i.e. the interdigital area and coronary band on the feet, and the tongue, contained the highest level of viral RNA, indicating the predominant tissue sites of viral infection and amplification during the acute stage of infection. Clearance of viral RNA from most of the tissues occurred relatively rapidly and the rate of clearance was largely independent of the level of viral RNA. The viral RNA load in most of the tissues declined slower than in serum, in which viral clearance is rapid. Beyond 28 days p.i., a proportion of pharyngeal region tissues (soft palate, pharynx, tonsil and mandibular lymph node) from infected animals still contained a detectable level of viral RNA, while viral RNA in non-pharyngeal region tissues was generally only detectable for variable periods ranging from 4 to 14 days p.i. The presence of viral RNA in dorsal soft palate tissue had a good correlation with the presence of infectious virus in oesophageal-pharyngeal fluid (OP-fluid) samples, a finding indicative of the specific tissue sites of FMDV persistence.

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2004-09-01
2019-10-15
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References

  1. Alexandersen, S. & Donaldson, A. I. ( 2002; ). Further studies to quantify the dose of natural aerosols of foot-and-mouth disease virus for pigs. Epidemiol Infect 128, 313–323.
    [Google Scholar]
  2. Alexandersen, S., Oleksiewicz, M. B. & Donaldson, A. I. ( 2001; ). The early pathogenesis of foot-and-mouth disease in pigs infected by contact: a quantitative time-course study using TaqMan RT-PCR. J Gen Virol 82, 747–755.
    [Google Scholar]
  3. Alexandersen, S., Brotherhood, I. & Donaldson, A. I. ( 2002a; ). Natural aerosol transmission of foot-and-mouth disease virus to pigs: minimal infectious dose for strain O1 Lausanne. Epidemiol Infect 128, 301–312.
    [Google Scholar]
  4. Alexandersen, S., Zhang, Z. & Donaldson, A. I. ( 2002b; ). Aspects of the persistence of foot-and-mouth disease virus in animals - the carrier problem. Microbes Infect 4, 1099–1110.[CrossRef]
    [Google Scholar]
  5. Alexandersen, S., Zhang, Z., Reid, S. M., Hutchings, G. H. & Donaldson, A. I. ( 2002c; ). Quantities of infectious virus and viral RNA recovered from sheep and cattle experimentally infected with foot-and-mouth disease virus O UK 2001. J Gen Virol 83, 1915–1923.
    [Google Scholar]
  6. Alexandersen, S., Quan, M., Murphy, C., Knight, J. & Zhang, Z. ( 2003a; ). Studies of quantitative parameters of virus excretion and transmission in pigs and cattle experimentally infected with foot-and-mouth disease virus. J Comp Pathol 129, 268–282.[CrossRef]
    [Google Scholar]
  7. Alexandersen, S., Zhang, Z., Donaldson, A. I. & Garland, A. J. ( 2003b; ). The pathogenesis and diagnosis of foot-and-mouth disease. J Comp Pathol 129, 1–36.[CrossRef]
    [Google Scholar]
  8. Bachrach, H. L. ( 1968; ). Foot-and-mouth disease. Annu Rev Microbiol 22, 201–244.[CrossRef]
    [Google Scholar]
  9. Bergmann, I. E., Malirat, V., Auge de Mello, P. & Gomes, I. ( 1996; ). Detection of foot-and-mouth disease viral sequences in various fluids and tissues during persistence of the virus in cattle. Am J Vet Res 57, 134–137.
    [Google Scholar]
  10. Burrows, R. ( 1966; ). Studies on the carrier state of cattle exposed to foot-and-mouth disease virus. J Hyg (Lond) 64, 81–90.[CrossRef]
    [Google Scholar]
  11. Burrows, R., Mann, J. A., Greig, A., Chapman, W. G. & Goodridge, D. ( 1971; ). The growth and persistence of foot-and-mouth disease virus in the bovine mammary gland. J Hyg (Lond) 69, 307–321.[CrossRef]
    [Google Scholar]
  12. Dawe, P. S., Sorensen, K., Ferris, N. P., Barnett, I. T., Armstrong, R. M. & Knowles, N. J. ( 1994; ). Experimental transmission of foot-and-mouth disease virus from carrier African buffalo (Syncerus caffer) to cattle in Zimbabwe. Vet Rec 134, 211–215.[CrossRef]
    [Google Scholar]
  13. Hedger, R. S. & Condy, J. B. ( 1985; ). Transmission of foot-and-mouth disease from African buffalo virus carriers to bovines. Vet Rec 117, 205.
    [Google Scholar]
  14. McVicar, J. W. & Sutmoller, P. ( 1969; ). The epizootiological importance of foot-and-mouth disease carriers. II. The carrier status of cattle exposed to foot-and-mouth disease following vaccination with an oil adjuvant inactivated virus vaccine. Arch Gesamte Virusforsch 26, 217–224.[CrossRef]
    [Google Scholar]
  15. Oleksiewicz, M. B., Donaldson, A. I. & Alexandersen, S. ( 2001; ). Development of a novel real-time RT-PCR assay for quantitation of foot-and-mouth disease virus in diverse porcine tissues. J Virol Methods 92, 23–35.[CrossRef]
    [Google Scholar]
  16. Prato Murphy, M. L., Meyer, R. F., Mebus, C., Schudel, A. A. & Rodriguez, M. ( 1994; ). Analysis of sites of foot and mouth disease virus persistence in carrier cattle via the polymerase chain reaction. Arch Virol 136, 299–307.[CrossRef]
    [Google Scholar]
  17. Prato Murphy, M. L., Forsyth, M. A., Belsham, G. J. & Salt, J. S. ( 1999; ). Localization of foot-and-mouth disease virus RNA by in situ hybridization within bovine tissues. Virus Res 62, 67–76.[CrossRef]
    [Google Scholar]
  18. Reid, S. M., Ferris, N. P., Hutchings, G. H., Zhang, Z., Belsham, G. J. & Alexandersen, S. ( 2001; ). Diagnosis of foot-and-mouth disease by real-time fluorogenic PCR assay. Vet Rec 149, 621–623.[CrossRef]
    [Google Scholar]
  19. Reid, S., Ferris, N. P., Hutchings, G. H., Zhang, Z., Belsham, G. J. & Alexandersen, S. ( 2002; ). Detection of all seven serotypes of foot-and-mouth disease virus by real-time, fluorogenic reverse transcription polymerase chain reaction assay. J Virol Methods 105, 67–80.[CrossRef]
    [Google Scholar]
  20. Reid, S. M., Grierson, S. S., Ferris, N. P., Hutchings, G. H. & Alexandersen, S. ( 2003; ). Evaluation of automated RT-PCR to accelerate the laboratory diagnosis of foot-and-mouth disease virus. J Virol Methods 107, 129–139.[CrossRef]
    [Google Scholar]
  21. Rossi, M. S., Sadir, A. M., Schudel, A. A. & Palma, E. L. ( 1988; ). Detection of foot-and-mouth disease virus with DNA probes in bovine esophageal-pharyngeal fluids. Arch Virol 99, 67–74.[CrossRef]
    [Google Scholar]
  22. Salt, J. S. ( 1993; ). The carrier state in foot and mouth disease–an immunological review. Br Vet J 149, 207–223.[CrossRef]
    [Google Scholar]
  23. Salt, J. S. ( 1998; ). Persistent infection with foot-and-mouth disease virus. Top Trop Virol 1, 77–129.
    [Google Scholar]
  24. Snowdon, W. A. ( 1966; ). Growth of foot-and-mouth disease virus in monolayer cultures of calf thyroid cells. Nature 210, 1079–1080.[CrossRef]
    [Google Scholar]
  25. Straver, P. J., Bool, P. H., Claessens, A. M. & van Bekkum, J. G. ( 1970; ). Some properties of carrier strains of foot-and-mouth disease virus. Arch Gesamte Virusforsch 29, 113–126.[CrossRef]
    [Google Scholar]
  26. Sutmoller, P. & Gaggero, A. ( 1965; ). Foot-and-mouth disease carriers. Vet Rec 77, 968–969.
    [Google Scholar]
  27. Woodbury, E. L., Ilott, M. C., Brown, C. C. & Salt, J. S. ( 1995; ). Optimization of an in situ hybridization technique for the detection of foot-and-mouth disease virus in bovine tissues using the digoxigenin system. J Virol Methods 51, 89–93.[CrossRef]
    [Google Scholar]
  28. Zhang, Z. & Kitching, P. ( 2000; ). A sensitive method for the detection of foot and mouth disease virus by in situ hybridisation using biotin-labelled oligodeoxynucleotides and tyramide signal amplification. J Virol Methods 88, 187–192.[CrossRef]
    [Google Scholar]
  29. Zhang, Z. D. & Kitching, R. P. ( 2001; ). The localization of persistent foot and mouth disease virus in the epithelial cells of the soft palate and pharynx. J Comp Pathol 124, 89–94.[CrossRef]
    [Google Scholar]
  30. Zhang, Z. & Alexandersen, S. ( 2003; ). Detection of carrier cattle and sheep persistently infected with foot-and-mouth disease virus by a rapid real-time RT-PCR assay. J Virol Methods 111, 95–100.[CrossRef]
    [Google Scholar]
  31. Zhang, Z. D., Hutching, G., Kitching, P. & Alexandersen, S. ( 2002; ). The effects of gamma interferon on replication of foot-and-mouth disease virus in persistently infected bovine cells. Arch Virol 147, 2157–2167.[CrossRef]
    [Google Scholar]
  32. Zhang, Z., Murphy, C., Quan, M., Knight, J. & Alexandersen, S. ( 2004; ). Extent of reduction of foot-and-mouth disease virus RNA load in oesophageal–pharyngeal fluid after peak levels may be a critical determinant of virus persistence in infected cattle. J Gen Virol 85, 415–421.[CrossRef]
    [Google Scholar]
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