1887

Abstract

is the only endogenous retrovirus of whose infectious properties have been reported. Previous studies have shown an unexpected relationship between the gene encoding the putative envelope glycoprotein (Env) of and genes encoding the fusion protein of several baculoviruses. The fact that fusion proteins mediate membrane fusion suggests that Env of insect retroviruses might also have fusogenic properties. The results reported here indicate that Env mediates cell-to-cell fusion. Cleavage of the Env precursor was also studied; it is shown that this polypeptide is cleaved at a furin-like cleavage site. This is the first report that the -like gene of insect retroviruses encodes a fusion protein.

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2004-11-01
2020-01-21
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References

  1. Bayev, A. A., Jr, Lyubomirskaya, N. V., Dzhumagaliev, E. B., Ananiev, E. V., Amiantova, I. G. & Ilyin, Y. V. ( 1984; ). Structural organization of transposable element mdg4 from Drosophila melanogaster and a nucleotide sequence of its long terminal repeats. Nucleic Acids Res 12, 3707–3723.[CrossRef]
    [Google Scholar]
  2. Bénit, L., Calteau, A. & Heidmann, T. ( 2003; ). Characterization of the low-copy HERV-Fc family: evidence for recent integrations in primates of elements with coding envelope genes. Virology 312, 159–168.[CrossRef]
    [Google Scholar]
  3. Bès, C., Cerutti, M., Briant-Longuet, L. & 9 other authors ( 2001; ). The chimeric mouse-human anti-CD4 Fab 13B8.2 expressed in baculovirus inhibits both antigen presentation and HIV-1 promoter activation. Hum Antibodies 10, 67–76.
    [Google Scholar]
  4. Beyer, W. R., Pöpplau, D., Garten, W., von Laer, D. & Lenz, O. ( 2003; ). Endoproteolytic processing of the lymphocytic choriomeningitis virus glycoprotein by the subtilase SKI-1/S1P. J Virol 77, 2866–2872.[CrossRef]
    [Google Scholar]
  5. Carlson, J., Lyon, M., Bishop, J. & 7 other authors ( 2003; ). Chromosomal distribution of endogenous Jaagsiekte sheep retrovirus proviral sequences in the sheep genome. J Virol 77, 9662–9668.[CrossRef]
    [Google Scholar]
  6. Chalvet, F., Teysset, L., Terzian, C., Prud'homme, N., Santamaria, P., Bucheton, A. & Pélisson, A. ( 1999; ). Proviral amplification of the Gypsy endogenous retrovirus of Drosophila melanogaster involves env-independent invasion of the female germline. EMBO J 18, 2659–2669.[CrossRef]
    [Google Scholar]
  7. Czauderna, F., Fischer, N., Boller, K., Kurth, R. & Tönjes, R. R. ( 2000; ). Establishment and characterization of molecular clones of porcine endogenous retroviruses replicating on human cells. J Virol 74, 4028–4038.[CrossRef]
    [Google Scholar]
  8. Felgner, P. L. & Ringold, G. M. ( 1989; ). Cationic liposome-mediated transfection. Nature 337, 387–388.[CrossRef]
    [Google Scholar]
  9. Gaymard, F., Cerutti, M., Horeau, C., Lemaillet, G., Urbach, S., Ravallec, M., Devauchelle, G., Sentenac, H. & Thibaud, J.-B. ( 1996; ). The baculovirus/insect cell system as an alternative to Xenopus oocytes. First characterization of the AKT1 K+ channel from Arabidopsis thaliana. J Biol Chem 271, 22863–22870.[CrossRef]
    [Google Scholar]
  10. Heinkelein, M., Rammling, M., Juretzek, T., Lindemann, D. & Rethwilm, A. ( 2003; ). Retrotransposition and cell-to-cell transfer of foamy viruses. J Virol 77, 11855–11858.[CrossRef]
    [Google Scholar]
  11. IJkel, W. F. J., Westenberg, M., Goldbach, R. W., Blissard, G. W., Vlak, J. M. & Zuidema, D. ( 2000; ). A novel baculovirus envelope fusion protein with a proprotein convertase cleavage site. Virology 275, 30–41.[CrossRef]
    [Google Scholar]
  12. Kim, A., Terzian, C., Santamaria, P., Pélisson, A., Prud'homme, N. & Bucheton, A. ( 1994; ). Retroviruses in invertebrates: the gypsy retrotransposon is apparently an infectious retrovirus of Drosophila melanogaster. Proc Natl Acad Sci U S A 91, 1285–1289.[CrossRef]
    [Google Scholar]
  13. Lenz, O., ter Meulen, J., Klenk, H.-D., Seidah, N. G. & Garten, W. ( 2001; ). The Lassa virus glycoprotein precursor GP-C is proteolytically processed by subtilase SKI-1/S1P. Proc Natl Acad Sci U S A 98, 12701–12705.[CrossRef]
    [Google Scholar]
  14. Löwer, R., Löwer, J. & Kurth, R. ( 1996; ). The viruses in all of us: characteristics and biological significance of human endogenous retrovirus sequences. Proc Natl Acad Sci U S A 93, 5177–5184.[CrossRef]
    [Google Scholar]
  15. Malik, H. S., Henikoff, S. & Eickbush, T. H. ( 2000; ). Poised for contagion: evolutionary origins of the infectious abilities of invertebrate retroviruses. Genome Res 10, 1307–1318.[CrossRef]
    [Google Scholar]
  16. Misseri, Y., Labesse, G., Bucheton, A. & Terzian, C. ( 2003; ). Comparative sequence analysis and predictions for the envelope glycoproteins of insect endogenous retroviruses. Trends Microbiol 11, 253–256.[CrossRef]
    [Google Scholar]
  17. Monsma, S. A., Oomens, A. G. P. & Blissard, G. W. ( 1996; ). The GP64 envelope fusion protein is an essential baculovirus protein required for cell-to-cell transmission of infection. J Virol 70, 4607–4616.
    [Google Scholar]
  18. Niebert, M. & Tönjes, R. R. ( 2003; ). Molecular cloning and functional characterization of infectious PERV and development of diagnostic tests. Curr Top Microbiol Immunol 278, 217–237.
    [Google Scholar]
  19. O'Reilly, D. R., Miller, L. K. & Luckow, V. A. ( 1992; ). Baculovirus Expression Vectors: a Laboratory Manual. New York: W. H. Freeman.
  20. Pearson, M. N., Russell, R. L. Q. & Rohrmann, G. F. ( 2002; ). Functional analysis of a conserved region of the baculovirus envelope fusion protein, LD130. Virology 304, 81–88.[CrossRef]
    [Google Scholar]
  21. Pélisson, A., Song, S. U., Prud'homme, N., Smith, P. A., Bucheton, A. & Corces, V. G. ( 1994; ). Gypsy transposition correlates with the production of a retroviral envelope-like protein under the tissue-specific control of the Drosophila flamenco gene. EMBO J 13, 4401–4411.
    [Google Scholar]
  22. Prud'homme, N., Gans, M., Masson, M., Terzian, C. & Bucheton, A. ( 1995; ). Flamenco, a gene controlling the gypsy retrovirus of Drosophila melanogaster. Genetics 139, 697–711.
    [Google Scholar]
  23. Rohrmann, G. F. & Karplus, P. A. ( 2001; ). Relatedness of baculovirus and gypsy retrotransposon envelope proteins. BMC Evol Biol 1, 1.[CrossRef]
    [Google Scholar]
  24. Song, S. U., Gerasimova, T., Kurkulos, M., Boeke, J. D. & Corces, V. G. ( 1994; ). An env-like protein encoded by a Drosophila retroelement: evidence that gypsy is an infectious retrovirus. Genes Dev 8, 2046–2057.[CrossRef]
    [Google Scholar]
  25. Terzian, C., Pélisson, A. & Bucheton, A. ( 2001; ). Evolution and phylogeny of insect endogenous retroviruses. BMC Evol Biol 1, 3.[CrossRef]
    [Google Scholar]
  26. Teysset, L., Burns, J. C., Shike, H., Sullivan, B. L., Bucheton, A. & Terzian, C. ( 1998; ). A Moloney murine leukemia virus-based retroviral vector pseudotyped by the insect retroviral gypsy envelope can infect Drosophila cells. J Virol 72, 853–856.
    [Google Scholar]
  27. Tönjes, R. R., Löwer, R., Boller, K. & 12 other authors ( 1996; ). HERV-K: the biologically most active human endogenous retrovirus family. J Acquir Immune Defic Syndr Hum Retrovirol 13 (Suppl. 1), S261–S267.[CrossRef]
    [Google Scholar]
  28. Vincent, M. J., Sanchez, A. J., Erickson, B. R., Basak, A., Chretien, M., Seidah, N. G. & Nichol, S. T. ( 2003; ). Crimean-Congo hemorrhagic fever virus glycoprotein proteolytic processing by subtilase SKI-1. J Virol 77, 8640–8649.[CrossRef]
    [Google Scholar]
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