1887

Abstract

The vaccinia virus (VACV) N1 protein is an intracellular virulence factor that has a Bcl-2-like structure and inhibits both apoptosis and signalling from the interleukin 1 receptor, leading to nuclear factor kappa B activation. Here, we investigated the immune response to intranasal infection with a virus lacking the gene (vΔN1L) compared with control viruses expressing . Data presented show that deletion of did not affect the proportion of CD4 and CD8 T cells infiltrating the lungs or the cytotoxic T-cell activity of these cells. However, vΔN1L induced an increased local natural killer cell activity between days 4 and 6 post-infection. In addition, in the absence of N1 the host inflammatory infiltrate was characterized by a reduced proportion of lymphocytes bearing the early activation marker CD69. Notably, there was a good correlation between the level of CD69 expression and weight loss. The implications of these findings are discussed.

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2008-11-01
2020-09-27
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