1887

Abstract

To investigate whether foot-and-mouth disease virus (FMDV) RNA loads in oesophageal–pharyngeal fluid (OP-fluid) in the early course of infection is related to the outcome of virus persistence, viral RNA in OP-fluid samples from cattle experimentally infected with FMDV type O was quantitatively analysed by using a quantitative real-time RT-PCR. Viral RNA was detected within 24 h post-infection (p.i.) in all infected animals. Rapid virus replication led to peak levels of viral RNA load by 30–53 h p.i., and then the load declined at various rates. In some animals (=12, so-called non-carriers) viral RNA became undetectable between 7 and 18 days p.i. In contrast, in persistently infected animals (=12, so-called carriers) viral RNA persisted in OP-fluid samples at detectable levels beyond 28 days p.i. Analysis of early viral decay/clearance and virus clearance half-life in OP-fluid samples showed that the extent of reduction of viral RNA in OP-fluid samples immediately following peak levels is a critical determinant of the outcome of FMDV persistence.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.19538-0
2004-02-01
2021-10-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/85/2/vir850415.html?itemId=/content/journal/jgv/10.1099/vir.0.19538-0&mimeType=html&fmt=ahah

References

  1. Alexandersen S., Donaldson A. I. 2002; Further studies to quantify the dose of natural aerosols of foot-and-mouth disease virus for pigs. Epidemiol Infect 128:313–323
    [Google Scholar]
  2. Alexandersen S., Oleksiewicz M. B., Donaldson A. I. 2001; The early pathogenesis of foot-and-mouth disease in pigs infected by contact: a quantitative time course study using TaqMan RT-PCR. J Gen Virol 82:747–755
    [Google Scholar]
  3. Alexandersen S., Zhang Z., Donaldson A. I. 2002a; Aspects of the persistence of foot-and-mouth disease virus in animals – the carrier problem. Microbes Infect 4:1099–1110
    [Google Scholar]
  4. Alexandersen S., Zhang Z., Reid S. M., Hutchings G. H., Donaldson A. I. 2002b; Quantities of infectious virus and viral RNA recovered from sheep and cattle experimentally infected with foot-and-mouth disease virus O UK 2001. J Gen Virol 83:1915–1923
    [Google Scholar]
  5. Alexandersen S., Quan M., Murphy C., Knight J., Zhang Z. 2003a; Studies of quantitative parameters of virus excretion and transmission in pigs and cattle experimentally infected with foot-and-mouth disease virus. J Comp Pathol 129:268–282
    [Google Scholar]
  6. Alexandersen S., Zhang Z., Donaldson A. I., Garland A. J. 2003b; The pathogenesis and diagnosis of foot-and-mouth disease. J Comp Pathol 129:1–36
    [Google Scholar]
  7. Burrows R., Mann J. A., Greig A., Chapman W. G., Goodridge D. 1971; The growth and persistence of foot-and-mouth disease virus in the bovine mammary gland. J Hyg (Lond) 69:307–321
    [Google Scholar]
  8. McVicar J. W., Sutmoller P. 1969; The epizootiological importance of foot-and-mouth disease carriers. II. The carrier status of cattle exposed to foot-and-mouth disease following vaccination with an oil adjuvant inactivated virus vaccine. Arch Gesamte Virusforsch 26:217–224
    [Google Scholar]
  9. Oleksiewicz M. B., Donaldson A. I., Alexandersen S. 2001; Development of a novel real-time RT-PCR assay for quantitation of foot-and-mouth disease virus in diverse porcine tissues. J Virol Methods 92:23–35
    [Google Scholar]
  10. Prato Murphy M. L., Meyer R. F., Mebus C., Schudel A. A., Rodriguez M. 1994; Analysis of sites of foot and mouth disease virus persistence in carrier cattle via the polymerase chain reaction. Arch Virol 136:299–307
    [Google Scholar]
  11. Reid S., Ferris N., Hutchings G., Zhang Z., Belsham G., Alexandersen S. 2002; Detection of all seven serotypes of foot-and-mouth disease virus by real-time, fluorogenic reverse transcription polymerase chain reaction assay. J Virol Methods 105:67–80
    [Google Scholar]
  12. Salt J. S. 1993; The carrier state in foot and mouth disease – an immunological review. Br Vet J 149:207–223
    [Google Scholar]
  13. Snowdon W. A. 1966; Growth of foot-and-mouth disease virus in monolayer cultures of calf thyroid cells. Nature 210:1079–1080
    [Google Scholar]
  14. Sutmoller P., Gaggero A. 1965; Foot-and-mouth diseases carriers. Vet Rec 77:968–969
    [Google Scholar]
  15. Zhang Z. D., Kitching R. P. 2001; The localization of persistent foot and mouth disease virus in the epithelial cells of the soft palate and pharynx. J Comp Pathol 124:89–94
    [Google Scholar]
  16. Zhang Z., Alexandersen S. 2003; Detection of carrier cattle and sheep persistently infected with foot-and-mouth disease virus by a rapid real-time RT-PCR assay. J Virol Methods 111:95–100
    [Google Scholar]
  17. Zhang Z. D., Hutching G., Kitching P., Alexandersen S. 2002; The effects of gamma interferon on replication of foot-and-mouth disease virus in persistently infected bovine cells. Arch Virol 147:2157–2167
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.19538-0
Loading
/content/journal/jgv/10.1099/vir.0.19538-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error