1887

Abstract

Blood monocytes infected with pseudorabies virus (PRV), a swine alphaherpesvirus, are not eliminated efficiently by antibody-dependent immunity and may occasionally transport PRV to the pregnant uterus of vaccinated animals. This study examines the long-term fate of PRV-infected monocytes cultivated in the presence of porcine PRV-specific antibodies. All monocytes were infected and expressed viral late proteins, and 30 % of PRV-infected monocytes cultivated with PRV-specific antibodies survived up to 194 h post-infection (p.i.), the end of the experiment (compared to 0 % for cells cultivated with PRV-negative antibodies). Of these surviving cells, ±75 % no longer expressed microscopically detectable viral late proteins from 144 h p.i. onwards. Remarkably, monocytes infected with a PRV gB-null virus did not survive in the presence of PRV-specific antibodies. These data suggest that PRV-specific antibodies suppress viral protein levels in infected monocytes, perhaps helping the virus to persist and reach internal organs in vaccinated animals.

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2003-11-01
2020-01-21
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References

  1. Andries, K., Pensaert, M. B. & Vandeputte, J. ( 1978; ). Effect of experimental infection with pseudorabies (Aujeszky's disease) virus on pigs with maternal immunity from vaccinated sows. Am J Vet Med 39, 1282–1285.
    [Google Scholar]
  2. Chanas, A. C., Ellis, D. S., Stamford, S. & Gould, E. A. ( 1982; ). The interaction of monoclonal antibodies directed against envelope glycoprotein E1 of Sindbis virus with virus-infected cells. Antiviral Res 2, 191–202.[CrossRef]
    [Google Scholar]
  3. Favoreel, H. W., Nauwynck, H. J. & Pensaert, M. B. ( 1999a; ). Role of the cytoplasmic tail of gE in antibody-induced redistribution of viral glycoproteins expressed on pseudorabies virus-infected cells. Virology 259, 141–147.[CrossRef]
    [Google Scholar]
  4. Favoreel, H. W., Nauwynck, H. J., Halewyck, H. M., Van Oostveldt, P., Mettenleiter, T. C. & Pensaert, M. B. ( 1999b; ). Antibody-induced endocytosis of viral glycoproteins and major histocompatibility complex class I on pseudorabies virus-infected monocytes. J Gen Virol 80, 1283–1291.
    [Google Scholar]
  5. Favoreel, H. W., Van Minnebruggen, G., Nauwynck, H. J., Enquist, L. W. & Pensaert, M. B. ( 2002; ). A tyrosine-based motif in the cytoplasmic tail of pseudorabies virus glycoprotein B is important for both antibody-induced internalization of viral glycoproteins and efficient cell-to-cell spread. J Virol 76, 6845–6851.[CrossRef]
    [Google Scholar]
  6. Griffin, D., Levine, B., Tyor, W., Ubol, S. & Despres, P. ( 1997; ). The role of antibody in recovery from alphavirus encephalitis. Immunol Rev 159, 155–161.[CrossRef]
    [Google Scholar]
  7. Griffin, D. E., Ubol, S., Despres, P., Kimura, T. & Byrnes, A. ( 2001; ). Role of antibodies in controlling alphavirus infection of neurons. Curr Top Microbiol Immunol 260, 191–200.
    [Google Scholar]
  8. Guidotti, L. G. & Chisari, F. V. ( 2000; ). Cytokine-mediated control of viral infections. Virology 273, 221–227.[CrossRef]
    [Google Scholar]
  9. Levine, B., Hardwick, J. M., Trapp, B. D., Crawford, T. O., Bollinger, R. C. & Griffin, D. E. ( 1991; ). Antibody-mediated clearance of alphaherpesvirus infection from neurons. Science 254, 856–860.[CrossRef]
    [Google Scholar]
  10. Nauwynck, H. J. & Pensaert, M. B. ( 1992; ). Abortion induced by cell-associated pseudorabies virus in vaccinated sows. Am J Vet Res 53, 489–493.
    [Google Scholar]
  11. Nauwynck, H. J. & Pensaert, M. B. ( 1995a; ). Cell-free and cell-associated viremia in pigs after oronasal infection with Aujeszky's disease virus. Vet Microbiol 43, 307–314.[CrossRef]
    [Google Scholar]
  12. Nauwynck, H. J. & Pensaert, M. B. ( 1995b; ). Effect of specific antibodies on the cell-associated spread of pseudorabies virus in monolayers of different cell types. Arch Virol 140, 1137–1146.[CrossRef]
    [Google Scholar]
  13. Oakes, J. E. & Lausch, R. N. ( 1984; ). Monoclonal antibodies suppress replication of herpes simplex virus type 1 in trigeminal ganglia. J Virol 51, 656–661.
    [Google Scholar]
  14. Pensaert, M. B. & Kluge, J. P. ( 1989; ). Pseudorabies Virus (Aujeszky's Disease). In Virus Infections of Porcines, pp. 39–64. Edited by M. B. Pensaert. Amsterdam: Elsevier.
  15. Rauh, I. & Mettenleiter, T. C. ( 1991; ). Pseudorabies virus glycoproteins gII and gp50 are essential for virus penetration. J Virol 65, 5348–5356.
    [Google Scholar]
  16. Rizvi, S. M. & Raghavan, M. ( 2003; ). Responses of herpes simplex virus type 1-infected cells to the presence of extracellular antibodies: gE-dependent glycoprotein capping and enhancement in cell-to-cell spread. J Virol 77, 701–708.[CrossRef]
    [Google Scholar]
  17. Sissons, J. G. & Oldstone, M. B. ( 1980; ). Antibody-mediated destruction of virus-infected cells. Adv Immunol 29, 209–260.
    [Google Scholar]
  18. Ubol, S., Levine, B., Lee, S.-H., Greenspan, N. S. & Griffin, D. E. ( 1995; ). Roles of immunoglobulin valency and the heavy-chain constant domain in antibody-mediated downregulation of Sindbis virus replication in persistently infected neurons. J Virol 69, 1990–1993.
    [Google Scholar]
  19. Van de Walle, G. R., Favoreel, H. W., Nauwynck, H. J., Van Oostveldt, P. & Pensaert, M. B. ( 2001; ). Involvement of cellular cytoskeleton components in antibody-induced internalization of viral glycoproteins in pseudorabies virus-infected monocytes. Virology 288, 129–138.[CrossRef]
    [Google Scholar]
  20. Van de Walle, G. R., Favoreel, H. W., Nauwynck, H. J. & Pensaert, M. B. ( 2003a; ). Antibody-induced internalization of viral glycoproteins and gE–gI Fc receptor activity protect pseudorabies virus-infected monocytes from efficient complement-mediated lysis. J Gen Virol 84, 939–948.[CrossRef]
    [Google Scholar]
  21. Van de Walle, G. R., Favoreel, H. W., Nauwynck, H. J., Mettenleiter, T. C. & Pensaert, M. B. ( 2003b; ). Transmission of pseudorabies virus from immune-masked blood monocytes to endothelial cells. J Gen Virol 84, 629–637.[CrossRef]
    [Google Scholar]
  22. Wittmann, G., Jakubik, J. & Ahl, R. ( 1980; ). Multiplication and distribution of Aujeszky's disease (pseudorabies) virus in vaccinated and non-vaccinated pigs after intranasal infection. Arch Virol 66, 227–240.[CrossRef]
    [Google Scholar]
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