@article{mbs:/content/journal/jgv/10.1099/vir.0.19281-0, author = "Favoreel, Herman W. and Van de Walle, Gerlinde R. and Nauwynck, Hans J. and Mettenleiter, Thomas C. and Pensaert, Maurice B.", title = "Pseudorabies virus (PRV)-specific antibodies suppress intracellular viral protein levels in PRV-infected monocytes", journal= "Journal of General Virology", year = "2003", volume = "84", number = "11", pages = "2969-2973", doi = "https://doi.org/10.1099/vir.0.19281-0", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.19281-0", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "Blood monocytes infected with pseudorabies virus (PRV), a swine alphaherpesvirus, are not eliminated efficiently by antibody-dependent immunity and may occasionally transport PRV to the pregnant uterus of vaccinated animals. This study examines in vitro the long-term fate of PRV-infected monocytes cultivated in the presence of porcine PRV-specific antibodies. All monocytes were infected and expressed viral late proteins, and 30 % of PRV-infected monocytes cultivated with PRV-specific antibodies survived up to 194 h post-infection (p.i.), the end of the experiment (compared to 0 % for cells cultivated with PRV-negative antibodies). Of these surviving cells, ±75 % no longer expressed microscopically detectable viral late proteins from 144 h p.i. onwards. Remarkably, monocytes infected with a PRV gB-null virus did not survive in the presence of PRV-specific antibodies. These data suggest that PRV-specific antibodies suppress viral protein levels in infected monocytes, perhaps helping the virus to persist and reach internal organs in vaccinated animals.", }