1887

Abstract

Replication of tobacco mosaic virus (TMV) is connected with endoplasmic reticulum (ER)-associated membranes at early stages of infection. This study reports that TMV movement protein (MP)-specific protein kinases (PKs) associated with the ER of tobacco were capable of phosphorylating Thr in TMV MP. The MP-specific PKs with apparent molecular masses of about 45–50 kDa and 38 kDa were revealed by gel PK assays. Two types of mutations were introduced in TMV MP gene of wild-type TMV U1 genome to substitute Thr by neutral Ala or by negatively charged Asp. Mutation of Thr to Ala did not affect the size of necrotic lesions induced by the mutant virus in Xanthi nc. plants. Conversely, mutation of Thr to Asp mimicking Thr phosphorylation strongly inhibited cell-to-cell movement. The possible role of Thr phosphorylation in TMV MP function is discussed.

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2003-03-01
2019-12-15
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