1887

Abstract

Adenoviruses are promising vectors for human cancer gene therapy. However, the extensively used adenoviruses serotypes 2 and 5 (Ad2 and Ad5) from species C have a major disadvantage in being highly prevalent; thus, most adults have an immunity against the two viruses. Furthermore, the expression of coxsackievirus and adenovirus receptors for Ad2 and Ad5 varies in different cells. This study aims to identify adenovirus serotypes with specific tropism for endothelial cells and epithelial tumour cells. Comparison of the binding affinities of Ad31, Ad11, Ad5, Ad37, Ad4 and Ad41, belonging to species A–F, respectively, to established cell lines of hepatoma (HepG2), breast cancer (CAMA and MG7), prostatic cancer (DU145 and LNCaP) and laryngeal cancer (Hep2), as well as to endothelial cells (HMEC), was carried out by flow cytometric analysis. Ad11 from species B showed markedly higher binding affinity than Ad5 for the endothelial cell line and all carcinoma cell lines studied. Ad4 showed a specific binding affinity for hepatoma cells and laryneal carcinoma cells. The ability of Ad11, Ad4 and Ad5 to be expressed in hepatoma, breast cancer and endothelial cell lines was studied by immunostaining and S-labelling of viral proteins in infected cells. Ad11 and Ad4 manifested a higher proportion of infected cells and a higher degree of hexon expression than Ad5.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.18666-0
2003-03-01
2019-12-06
Loading full text...

Full text loading...

/deliver/fulltext/jgv/84/3/vir840687.html?itemId=/content/journal/jgv/10.1099/vir.0.18666-0&mimeType=html&fmt=ahah

References

  1. Ades, E. W., Candal, F. J., Swerlick, R. A., George, V. G., Summers, S., Bosse, D. C. & Lawley, T. J. ( 1992a; ). HMEC-1: establishment of an immortalized human microvascular endothelial cell line. J Invest Dermatol 99, 683–690.[CrossRef]
    [Google Scholar]
  2. Ades, E. W., Hierholzer, J. C., George, V., Black, J. & Candal, F. ( 1992b; ). Viral susceptibility of an immortalized human microvascular endothelial cell line. J Virol Methods 39, 83–90.[CrossRef]
    [Google Scholar]
  3. Adrian, T., Wadell, G., Hierholzer, J. C. & Wigand, R. ( 1986; ). DNA restriction analysis of adenovirus prototypes 1 to 41. Arch Virol 91, 277–290.[CrossRef]
    [Google Scholar]
  4. Albinsson, B. & Kidd, A. H. ( 1999; ). Adenovirus type 41 lacks an RGD αv-integrin binding motif on the penton base and undergoes delayed uptake in A549 cells. Virus Res 64, 125–136.[CrossRef]
    [Google Scholar]
  5. Arnberg, N., Edlund, K., Kidd, A. H. & Wadell, G. ( 2000; ). Adenovirus type 37 uses sialic acid as a cellular receptor. J Virol 74, 42–48.[CrossRef]
    [Google Scholar]
  6. Babich, A., Feldman, L. T., Nevins, J. R., Darnell, J. E., Jr & Weinberger, C. ( 1983; ). Effect of adenovirus on metabolism of specific host mRNAs: transport control and specific translational discrimination. Mol Cell Biol 3, 1212–1221.
    [Google Scholar]
  7. Bergelson, J. M., Cunningham, J. A., Droguett, G., Kurt-Jones, E. A., Krithivas, A., Hong, J. S., Horwitz, M. S., Crowell, R. L. & Finberg, R. W. ( 1997; ). Isolation of a common receptor for coxsackie B viruses and adenoviruses 2 and 5. Science 275, 1320–1323.[CrossRef]
    [Google Scholar]
  8. Chroboczek, J., Ruigrok, R. W. & Cusack, S. ( 1995; ). Adenovirus fiber. Curr Top Microbiol Immunol 199, 163–200.
    [Google Scholar]
  9. Cohen, C. J., Shieh, J. T., Pickles, R. J., Okegawa, T., Hsieh, J. T. & Bergelson, J. M. ( 2001; ). The coxsackievirus and adenovirus receptor is a transmembrane component of the tight junction. Proc Natl Acad Sci U S A 98, 15191–15196.[CrossRef]
    [Google Scholar]
  10. D'Ambrosio, E., Del Grosso, N., Chicca, A. & Midulla, M. ( 1982; ). Neutralizing antibodies against 33 human adenoviruses in normal children in Rome. J Hyg 89, 155–161.[CrossRef]
    [Google Scholar]
  11. Davison, E., Kirby, I., Elliott, T. & Santis, G. ( 1999; ). The human HLA-A*0201 allele, expressed in hamster cells, is not a high-affinity receptor for adenovirus type 5 fiber. J Virol 73, 4513–4517.
    [Google Scholar]
  12. Dechecchi, M. C., Tamanini, A., Bonizzato, A. & Cabrini, G. ( 2000; ). Heparan sulfate glycosaminoglycans are involved in adenovirus type 5 and 2–host cell interactions. Virology 268, 382–390.[CrossRef]
    [Google Scholar]
  13. Defer, C., Belin, M. T., Caillet-Boudin, M. L. & Boulanger, P. ( 1990; ). Human adenovirus–host cell interactions: comparative study with members of subgroups B and C. J Virol 64, 3661–3673.
    [Google Scholar]
  14. Friedman, H. M., Macarak, E. J., MacGregor, R. R., Wolfe, J. & Kefalides, N. A. ( 1981; ). Virus infection of endothelial cells. J Infect Dis 143, 266–273.[CrossRef]
    [Google Scholar]
  15. Haisma, H. J., Pinedo, H. M., Rijswijk, A. & 7 other authors ( 1999; ). Tumor-specific gene transfer via an adenoviral vector targeted to the pan-carcinoma antigen EpCAM. Gene Ther 6, 1469–1474.[CrossRef]
    [Google Scholar]
  16. Hautala, T., Grunst, T., Fabrega, A., Freimuth, P. & Welsh, M. J. ( 1998; ). An interaction between penton base and αv integrins plays a minimal role in adenovirus-mediated gene transfer to hepatocytes in vitro and in vivo. Gene Ther 5, 1259–1264.[CrossRef]
    [Google Scholar]
  17. Havenga, M. J., Lemckert, A. A., Ophorst, O. J. & 13 other authors ( 2002; ). Exploiting the natural diversity in adenovirus tropism for therapy and prevention of disease. J Virol 76, 4612–4620.[CrossRef]
    [Google Scholar]
  18. Hong, S. S., Karayan, L., Tournier, J., Curiel, D. T. & Boulanger, P. A. ( 1997; ). Adenovirus type 5 fiber knob binds to MHC class I α2 domain at the surface of human epithelial and B lymphoblastoid cells. EMBO J 16, 2294–2306.[CrossRef]
    [Google Scholar]
  19. Kim, J. S., Jeon, H. M., Im, S. A. & Lee, S. N. ( 1999; ). Enhanced adenoviral transduction efficiency in HER-2/neu-overexpressing human breast cancer cells not induced by an integrin pathway. Oncol Rep 6, 1237–1242.
    [Google Scholar]
  20. Kim, M., Zinn, K., Barnett, B., Sumerel, L., Krasnykh, V., Curiel, D. & Douglas, J. ( 2002; ). The therapeutic efficacy of adenoviral vectors for cancer gene therapy is limited by a low level of primary adenovirus receptors on tumour cells. Eur J Cancer 38, 1917.[CrossRef]
    [Google Scholar]
  21. Krasnykh, V. N., Mikheeva, G. V., Douglas, J. T. & Curiel, D. T. ( 1996; ). Generation of recombinant adenovirus vectors with modified fibers for altering viral tropism. J Virol 70, 6839–6846.
    [Google Scholar]
  22. Legrand, V., Spehner, D., Schlesinger, Y., Settelen, N., Pavirani, A. & Mehtali, M. ( 1999; ). Fiberless recombinant adenoviruses: virus maturation and infectivity in the absence of fiber. J Virol 73, 907–919.
    [Google Scholar]
  23. Li, D., Duan, L., Freimuth, P. & O'Malley, B. W., Jr ( 1999a; ). Variability of adenovirus receptor density influences gene transfer efficiency and therapeutic response in head and neck cancer. Clin Cancer Res 5, 4175–4181.
    [Google Scholar]
  24. Li, Y., Pong, R. C., Bergelson, J. M., Hall, M. C., Sagalowsky, A. I., Tseng, C. P., Wang, Z. & Hsieh, J. T. ( 1999b; ). Loss of adenoviral receptor expression in human bladder cancer cells: a potential impact on the efficacy of gene therapy. Cancer Res 59, 325–330.
    [Google Scholar]
  25. Mathias, P., Wickham, T., Moore, M. & Nemerow, G. ( 1994; ). Multiple adenovirus serotypes use αv integrins for infection. J Virol 68, 6811–6814.
    [Google Scholar]
  26. McDonald, D., Stockwin, L., Matzow, T., Blair Zajdel, M. E. & Blair, G. E. ( 1999; ). Coxsackie and adenovirus receptor (CAR)-dependent and major histocompatibility complex (MHC) class I-independent uptake of recombinant adenoviruses into human tumour cells. Gene Ther 6, 1512–1519.[CrossRef]
    [Google Scholar]
  27. Mei, Y. F., Lindman, K. & Wadell, G. ( 1998; ). Two closely related adenovirus genome types with kidney or respiratory tract tropism differ in their binding to epithelial cells of various origins. Virology 240, 254–266.[CrossRef]
    [Google Scholar]
  28. Miyazawa, N., Leopold, P. L., Hackett, N. R., Ferris, B., Worgall, S., Falck-Pedersen, E. & Crystal, R. G. ( 1999; ). Fiber swap between adenovirus subgroups B and C alters intracellular trafficking of adenovirus gene transfer vectors. J Virol 73, 6056–6065.
    [Google Scholar]
  29. Miyazawa, N., Crystal, R. G. & Leopold, P. L. ( 2001; ). Adenovirus serotype 7 retention in a late endosomal compartment prior to cytosol escape is modulated by fiber protein. J Virol 75, 1387–1400.[CrossRef]
    [Google Scholar]
  30. Pearson, A. S., Koch, P. E., Atkinson, N., Xiong, M., Finberg, R. W., Roth, J. A. & Fang, B. ( 1999; ). Factors limiting adenovirus-mediated gene transfer into human lung and pancreatic cancer cell lines. Clin Cancer Res 5, 4208–4213.
    [Google Scholar]
  31. Ramalingam, R., Rafii, S., Worgall, S., Hackett, N. R. & Crystal, R. G. ( 1999; ). Induction of endogenous genes following infection of human endothelial cells with an E1E4+ adenovirus gene transfer vector. J Virol 73, 10183–10190.
    [Google Scholar]
  32. Roelvink, P. W., Lizonova, A., Lee, J. G., Li, Y., Bergelson, J. M., Finberg, R. W., Brough, D. E., Kovesdi, I. & Wickham, T. J. ( 1998; ). The coxsackievirus–adenovirus receptor protein can function as a cellular attachment protein for adenovirus serotypes from subgroups A, C, D, E, and F. J Virol 72, 7909–7915.
    [Google Scholar]
  33. Roelvink, P. W., Mi Lee, G., Einfeld, D. A., Kovesdi, I. & Wickham, T. J. ( 1999; ). Identification of a conserved receptor-binding site on the fiber proteins of CAR-recognizing Adenoviridae. Science 286, 1568–1571.[CrossRef]
    [Google Scholar]
  34. Russell, W. C. ( 2000; ). Update on adenovirus and its vectors. J GenVirol 81, 2573–2604.
    [Google Scholar]
  35. Santis, G., Legrand, V., Hong, S. S. & 7 other authors ( 1999; ). Molecular determinants of adenovirus serotype 5 fibre binding to its cellular receptor CAR. J Gen Virol 80, 1519–1527.
    [Google Scholar]
  36. Segerman, A., Mei, Y. F. & Wadell, G. ( 2000; ). Adenovirus types 11p and 35p show high binding efficiencies for committed hematopoietic cell lines and are infective to these cell lines. J Virol 74, 1457–1467.[CrossRef]
    [Google Scholar]
  37. Shayakhmetov, D. M., Papayannopoulou, T., Stamatoyannopoulos, G. & Lieber, A. ( 2000; ). Efficient gene transfer into human CD34+ cells by a retargeted adenovirus vector. J Virol 74, 2567–2583.[CrossRef]
    [Google Scholar]
  38. Skog, J., Mei, Y. F. & Wadell, G. ( 2002; ). Adenovirus serotype 4p and 11p are efficiently expressed in cell lines of neural tumour origin. J Gen Virol 83, 1299–1309.
    [Google Scholar]
  39. Stevenson, S. C., Rollence, M., White, B., Weaver, L. & McClelland, A. ( 1995; ). Human adenovirus serotypes 3 and 5 bind to two different cellular receptors via the fiber head domain. J Virol 69, 2850–2857.
    [Google Scholar]
  40. Wickham, T. J., Mathias, P., Cheresh, D. A. & Nemerow, G. R. ( 1993; ). Integrins αvβ3 and αvβ5 promote adenovirus internalization but not virus attachment. Cell 73, 309–319.[CrossRef]
    [Google Scholar]
  41. Yoder, S. S. & Berget, S. M. ( 1985; ). Posttranscriptional control of DHFR gene expression during adenovirus 2 infection. J Virol 54, 72–77.
    [Google Scholar]
  42. Zabner, J., Couture, L. A., Smith, A. E. & Welsh, M. J. ( 1994; ). Correction of cAMP-stimulated fluid secretion in cystic fibrosis airway epithelia: efficiency of adenovirus-mediated gene transfer in vitro. Hum Gene Ther 5, 585–593.[CrossRef]
    [Google Scholar]
  43. Zabner, J., Chillon, M., Grunst, T., Moninger, T. O., Davidson, B. L., Gregory, R. & Armentano, D. ( 1999; ). A chimeric type 2 adenovirus vector with a type 17 fiber enhances gene transfer to human airway epithelia. J Virol 73, 8689–8695.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.18666-0
Loading
/content/journal/jgv/10.1099/vir.0.18666-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error