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Journal of General Virology header logo Journal of General Virology

Volume 95, Issue 2

Whole genome analysis of epizootic hemorrhagic disease virus identified limited genome constellations and preferential reassortment No Access

Abstract

Epizootic hemorrhagic disease virus (EHDV) is a transmitted orbivirus that causes haemorrhagic disease in wild and domestic ruminants. A collection of 44 EHDV isolated from 2008 to 2012 was fully sequenced and analysed phylogenetically. Serotype 2 viruses were the dominant serotype all years except 2012 when serotype 6 viruses represented 63 % of the isolates. High genetic similarity (>94 % identity) between serotype 1 and 2 virus VP1, VP3, VP4, VP6, NS1, NS2 and NS3 segments prevented identification of reassortment events for these segments. Additionally, there was little genetic diversity (>96 % identity) within serotypes for VP2, VP5 and VP7. Preferential reassortment within the homologous serotype was observed for VP2, VP5 and VP7 segments for type 1 and type 2 viruses. In contrast, type 6 viruses were all reassortants containing VP2 and VP5 derived from an exotic type 6 with the remaining segments most similar to type 2 viruses. These results suggest that reassortment between type 1 and type 2 viruses requires conservation of the VP2, VP5 and VP7 segment constellation while type 6 viruses only require VP2 and VP5 and are restricted to type 2-lineage VP7. As type 6 VP2 and VP5 segments were exclusively identified in viruses with type 2-derived VP7, these results suggest functional complementation between type 2 and type 6 VP7 proteins.

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© 2014 SGM
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2014-02-01
2025-12-07

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References

  1. Allison A. B., Goekjian V. H., Potgieter A. C., Wilson W. C., Johnson D. J., Mertens P. P., Stallknecht D. E. 2010; Detection of a novel reassortant epizootic hemorrhagic disease virus (EHDV) in the USA containing RNA segments derived from both exotic (EHDV-6) and endemic (EHDV-2) serotypes. J Gen Virol 91:430–439 [View Article][PubMed]
     [Google Scholar]
  2. Anthony S. J., Maan N., Maan S., Sutton G., Attoui H., Mertens P. P. 2009a; Genetic and phylogenetic analysis of the core proteins VP1, VP3, VP4, VP6 and VP7 of epizootic haemorrhagic disease virus (EHDV). Virus Res 145:187–199 [View Article][PubMed]
     [Google Scholar]
  3. Anthony S. J., Maan N., Maan S., Sutton G., Attoui H., Mertens P. P. 2009b; Genetic and phylogenetic analysis of the non-structural proteins NS1, NS2 and NS3 of epizootic haemorrhagic disease virus (EHDV). Virus Res 145:211–219 [View Article][PubMed]
     [Google Scholar]
  4. Anthony S. J., Maan S., Maan N., Kgosana L., Bachanek-Bankowska K., Batten C., Darpel K. E., Sutton G., Attoui H., Mertens P. P. 2009c; Genetic and phylogenetic analysis of the outer-coat proteins VP2 and VP5 of epizootic haemorrhagic disease virus (EHDV): comparison of genetic and serological data to characterise the EHDV serogroup. Virus Res 145:200–210 [View Article][PubMed]
     [Google Scholar]
  5. Campbell C. H., St George T. D. 1986; A preliminary report of a comparison of epizootic haemorrhagic disease viruses from Australia with others from North America, Japan and Nigeria. Aust Vet J 63:233 [View Article][PubMed]
     [Google Scholar]
  6. Cheney I. W., Yamakawa M., Roy P., Mecham J. O., Wilson W. C. 1996; Molecular characterization of the segment 2 gene of epizootic hemorrhagic disease virus serotype 2: gene sequence and genetic diversity. Virology 224:555–560 [View Article][PubMed]
     [Google Scholar]
  7. Clavijo A., Sun F., Lester T., Jasperson D. C., Wilson W. C. 2010; An improved real-time polymerase chain reaction for the simultaneous detection of all serotypes of epizootic hemorrhagic disease virus. J Vet Diagn Invest 22:588–593 [View Article][PubMed]
     [Google Scholar]
  8. Couvillion C. E., Jenney E. W., Pearson J. E., Coker M. E. 1980; Survey for antibodies to viruses of bovine virus diarrhea, bluetongue, and epizootic hemorrhagic disease in hunter-killed mule deer in New Mexico. J Am Vet Med Assoc 177:790–791[PubMed]
     [Google Scholar]
  9. Holland J., Spindler K., Horodyski F., Grabau E., Nichol S., VandePol S. 1982; Rapid evolution of RNA genomes. Science 215:1577–1585 [View Article][PubMed]
     [Google Scholar]
  10. Jenkins G. M., Rambaut A., Pybus O. G., Holmes E. C. 2002; Rates of molecular evolution in RNA viruses: a quantitative phylogenetic analysis. J Mol Evol 54:156–165 [View Article][PubMed]
     [Google Scholar]
  11. Jessup D. A. 1985; Epidemiology of two orbiviruses in California’s native wild ruminants: preliminary report. Prog Clin Biol Res 178:53–65[PubMed]
     [Google Scholar]
  12. Lee V. H., Causey O. R., Moore D. L. 1974; Bluetongue and related viruses in Ibadan, Nigeria: isolation and preliminary identification of viruses. Am J Vet Res 35:1105–1108[PubMed]
     [Google Scholar]
  13. Mecham J. O., Dean V. C. 1988; Protein coding assignment for the genome of epizootic haemorrhagic disease virus. J Gen Virol 69:1255–1262 [View Article][PubMed]
     [Google Scholar]
  14. Mohammed M. E., Aradaib I. E., Mukhtar M. M., Ghalib H. W., Riemann H. P., Oyejide A., Osburn B. I. 1996; Application of molecular biological techniques for detection of epizootic hemorrhagic disease virus (EHDV-318) recovered from a sentinel calf in central Sudan. Vet Microbiol 52:201–208 [View Article][PubMed]
     [Google Scholar]
  15. Moore D. L. 1974; Bluetongue and related viruses in Ibadan, Nigeria: serologic comparison of bluetongue, epizootic hemorrhagic disease of deer, and Abadina (Palyam) viral isolates. Am J Vet Res 35:1109–1113[PubMed]
     [Google Scholar]
  16. Moore D. L., Kemp G. E. 1974; Bluetongue and related viruses in Ibadan, Nigeria: serologic studies of domesticated and wild animals. Am J Vet Res 35:1115–1120[PubMed]
     [Google Scholar]
  17. Murphy M. D., Howerth E. W., MacLachlan N. J., Stallknecht D. E. 2005; Genetic variation among epizootic hemorrhagic disease viruses in the southeastern United States: 1978–2001. Infect Genet Evol 5:157–165 [View Article][PubMed]
     [Google Scholar]
  18. Mutebi J.-P., Wang H., Li L., Bryant J. E., Barrett A. D. T. 2001; Phylogenetic and evolutionary relationships among yellow fever virus isolates in Africa. J Virol 75:6999–7008 [View Article][PubMed]
     [Google Scholar]
  19. Potgieter A. C., Page N. A., Liebenberg J., Wright I. M., Landt O., van Dijk A. A. 2009; Improved strategies for sequence-independent amplification and sequencing of viral double-stranded RNA genomes. J Gen Virol 90:1423–1432 [View Article][PubMed]
     [Google Scholar]
  20. Savini G., Afonso A., Mellor P., Aradaib I., Yadin H., Sanaa M., Wilson W., Monaco F., Domingo M. 2011; Epizootic haemorragic disease. Res Vet Sci 91:1–17 [View Article][PubMed]
     [Google Scholar]
  21. Shaw A. E., Ratinier M., Nunes S. F., Nomikou K., Caporale M., Golder M., Allan K., Hamers C., Hudelet P.other authors 2013; Reassortment between two serologically unrelated bluetongue virus strains is flexible and can involve any genome segment. J Virol 87:543–557 [View Article][PubMed]
     [Google Scholar]
  22. St George T. D., Cybinski D. H., Standfast H. A., Gard G. P., Della-Porta A. J. 1983; The isolation of five different viruses of the epizootic haemorrhagic disease of deer serogroup. Aust Vet J 60:216–217 [View Article][PubMed]
     [Google Scholar]
  23. Stallknecht D. E., Nettles V. F., Fletcher W. O., Erickson G. A. 1985; Enzootic vesicular stomatitis New Jersey type in an insular feral swine population. Am J Epidemiol 122:876–883[PubMed]
     [Google Scholar]
  24. Stallknecht D. E., Nettles V. F., Rollor E. A. III, Howerth E. W. 1995; Epizootic hemorrhagic disease virus and bluetongue virus serotype distribution in white-tailed deer in Georgia. J Wildl Dis 31:331–338 [View Article][PubMed]
     [Google Scholar]
  25. Yadin H., Brenner J., Bumbrov V., Oved Z., Stram Y., Klement E., Perl S., Anthony S., Maan S.other authors 2008; Epizootic haemorrhagic disease virus type 7 infection in cattle in Israel. Vet Rec 162:53–56 [View Article][PubMed]
     [Google Scholar]
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Whole genome analysis of epizootic hemorrhagic disease virus identified limited genome constellations and preferential reassortment
J. Gen. Virol. 95, 434 (2014); https://doi.org/10.1099/vir.0.059659-0
/content/journal/jgv/10.1099/vir.0.059659-0
/content/journal/jgv/10.1099/vir.0.059659-0
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