1887

Abstract

Epizootic hemorrhagic disease virus (EHDV) is a transmitted orbivirus that causes haemorrhagic disease in wild and domestic ruminants. A collection of 44 EHDV isolated from 2008 to 2012 was fully sequenced and analysed phylogenetically. Serotype 2 viruses were the dominant serotype all years except 2012 when serotype 6 viruses represented 63 % of the isolates. High genetic similarity (>94 % identity) between serotype 1 and 2 virus VP1, VP3, VP4, VP6, NS1, NS2 and NS3 segments prevented identification of reassortment events for these segments. Additionally, there was little genetic diversity (>96 % identity) within serotypes for VP2, VP5 and VP7. Preferential reassortment within the homologous serotype was observed for VP2, VP5 and VP7 segments for type 1 and type 2 viruses. In contrast, type 6 viruses were all reassortants containing VP2 and VP5 derived from an exotic type 6 with the remaining segments most similar to type 2 viruses. These results suggest that reassortment between type 1 and type 2 viruses requires conservation of the VP2, VP5 and VP7 segment constellation while type 6 viruses only require VP2 and VP5 and are restricted to type 2-lineage VP7. As type 6 VP2 and VP5 segments were exclusively identified in viruses with type 2-derived VP7, these results suggest functional complementation between type 2 and type 6 VP7 proteins.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.059659-0
2014-02-01
2019-09-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/95/2/434.html?itemId=/content/journal/jgv/10.1099/vir.0.059659-0&mimeType=html&fmt=ahah

References

  1. Allison A. B., Goekjian V. H., Potgieter A. C., Wilson W. C., Johnson D. J., Mertens P. P., Stallknecht D. E.. ( 2010; ). Detection of a novel reassortant epizootic hemorrhagic disease virus (EHDV) in the USA containing RNA segments derived from both exotic (EHDV-6) and endemic (EHDV-2) serotypes. . J Gen Virol 91:, 430–439. [CrossRef] [PubMed]
    [Google Scholar]
  2. Anthony S. J., Maan N., Maan S., Sutton G., Attoui H., Mertens P. P.. ( 2009a; ). Genetic and phylogenetic analysis of the core proteins VP1, VP3, VP4, VP6 and VP7 of epizootic haemorrhagic disease virus (EHDV). . Virus Res 145:, 187–199. [CrossRef] [PubMed]
    [Google Scholar]
  3. Anthony S. J., Maan N., Maan S., Sutton G., Attoui H., Mertens P. P.. ( 2009b; ). Genetic and phylogenetic analysis of the non-structural proteins NS1, NS2 and NS3 of epizootic haemorrhagic disease virus (EHDV). . Virus Res 145:, 211–219. [CrossRef] [PubMed]
    [Google Scholar]
  4. Anthony S. J., Maan S., Maan N., Kgosana L., Bachanek-Bankowska K., Batten C., Darpel K. E., Sutton G., Attoui H., Mertens P. P.. ( 2009c; ). Genetic and phylogenetic analysis of the outer-coat proteins VP2 and VP5 of epizootic haemorrhagic disease virus (EHDV): comparison of genetic and serological data to characterise the EHDV serogroup. . Virus Res 145:, 200–210. [CrossRef] [PubMed]
    [Google Scholar]
  5. Campbell C. H., St George T. D.. ( 1986; ). A preliminary report of a comparison of epizootic haemorrhagic disease viruses from Australia with others from North America, Japan and Nigeria. . Aust Vet J 63:, 233. [CrossRef] [PubMed]
    [Google Scholar]
  6. Cheney I. W., Yamakawa M., Roy P., Mecham J. O., Wilson W. C.. ( 1996; ). Molecular characterization of the segment 2 gene of epizootic hemorrhagic disease virus serotype 2: gene sequence and genetic diversity. . Virology 224:, 555–560. [CrossRef] [PubMed]
    [Google Scholar]
  7. Clavijo A., Sun F., Lester T., Jasperson D. C., Wilson W. C.. ( 2010; ). An improved real-time polymerase chain reaction for the simultaneous detection of all serotypes of epizootic hemorrhagic disease virus. . J Vet Diagn Invest 22:, 588–593. [CrossRef] [PubMed]
    [Google Scholar]
  8. Couvillion C. E., Jenney E. W., Pearson J. E., Coker M. E.. ( 1980; ). Survey for antibodies to viruses of bovine virus diarrhea, bluetongue, and epizootic hemorrhagic disease in hunter-killed mule deer in New Mexico. . J Am Vet Med Assoc 177:, 790–791.[PubMed]
    [Google Scholar]
  9. Holland J., Spindler K., Horodyski F., Grabau E., Nichol S., VandePol S.. ( 1982; ). Rapid evolution of RNA genomes. . Science 215:, 1577–1585. [CrossRef] [PubMed]
    [Google Scholar]
  10. Jenkins G. M., Rambaut A., Pybus O. G., Holmes E. C.. ( 2002; ). Rates of molecular evolution in RNA viruses: a quantitative phylogenetic analysis. . J Mol Evol 54:, 156–165. [CrossRef] [PubMed]
    [Google Scholar]
  11. Jessup D. A.. ( 1985; ). Epidemiology of two orbiviruses in California’s native wild ruminants: preliminary report. . Prog Clin Biol Res 178:, 53–65.[PubMed]
    [Google Scholar]
  12. Lee V. H., Causey O. R., Moore D. L.. ( 1974; ). Bluetongue and related viruses in Ibadan, Nigeria: isolation and preliminary identification of viruses. . Am J Vet Res 35:, 1105–1108.[PubMed]
    [Google Scholar]
  13. Mecham J. O., Dean V. C.. ( 1988; ). Protein coding assignment for the genome of epizootic haemorrhagic disease virus. . J Gen Virol 69:, 1255–1262. [CrossRef] [PubMed]
    [Google Scholar]
  14. Mohammed M. E., Aradaib I. E., Mukhtar M. M., Ghalib H. W., Riemann H. P., Oyejide A., Osburn B. I.. ( 1996; ). Application of molecular biological techniques for detection of epizootic hemorrhagic disease virus (EHDV-318) recovered from a sentinel calf in central Sudan. . Vet Microbiol 52:, 201–208. [CrossRef] [PubMed]
    [Google Scholar]
  15. Moore D. L.. ( 1974; ). Bluetongue and related viruses in Ibadan, Nigeria: serologic comparison of bluetongue, epizootic hemorrhagic disease of deer, and Abadina (Palyam) viral isolates. . Am J Vet Res 35:, 1109–1113.[PubMed]
    [Google Scholar]
  16. Moore D. L., Kemp G. E.. ( 1974; ). Bluetongue and related viruses in Ibadan, Nigeria: serologic studies of domesticated and wild animals. . Am J Vet Res 35:, 1115–1120.[PubMed]
    [Google Scholar]
  17. Murphy M. D., Howerth E. W., MacLachlan N. J., Stallknecht D. E.. ( 2005; ). Genetic variation among epizootic hemorrhagic disease viruses in the southeastern United States: 1978–2001. . Infect Genet Evol 5:, 157–165. [CrossRef] [PubMed]
    [Google Scholar]
  18. Mutebi J.-P., Wang H., Li L., Bryant J. E., Barrett A. D. T.. ( 2001; ). Phylogenetic and evolutionary relationships among yellow fever virus isolates in Africa. . J Virol 75:, 6999–7008. [CrossRef] [PubMed]
    [Google Scholar]
  19. Potgieter A. C., Page N. A., Liebenberg J., Wright I. M., Landt O., van Dijk A. A.. ( 2009; ). Improved strategies for sequence-independent amplification and sequencing of viral double-stranded RNA genomes. . J Gen Virol 90:, 1423–1432. [CrossRef] [PubMed]
    [Google Scholar]
  20. Savini G., Afonso A., Mellor P., Aradaib I., Yadin H., Sanaa M., Wilson W., Monaco F., Domingo M.. ( 2011; ). Epizootic haemorragic disease. . Res Vet Sci 91:, 1–17. [CrossRef] [PubMed]
    [Google Scholar]
  21. Shaw A. E., Ratinier M., Nunes S. F., Nomikou K., Caporale M., Golder M., Allan K., Hamers C., Hudelet P.. & other authors ( 2013; ). Reassortment between two serologically unrelated bluetongue virus strains is flexible and can involve any genome segment. . J Virol 87:, 543–557. [CrossRef] [PubMed]
    [Google Scholar]
  22. St George T. D., Cybinski D. H., Standfast H. A., Gard G. P., Della-Porta A. J.. ( 1983; ). The isolation of five different viruses of the epizootic haemorrhagic disease of deer serogroup. . Aust Vet J 60:, 216–217. [CrossRef] [PubMed]
    [Google Scholar]
  23. Stallknecht D. E., Nettles V. F., Fletcher W. O., Erickson G. A.. ( 1985; ). Enzootic vesicular stomatitis New Jersey type in an insular feral swine population. . Am J Epidemiol 122:, 876–883.[PubMed]
    [Google Scholar]
  24. Stallknecht D. E., Nettles V. F., Rollor E. A. III, Howerth E. W.. ( 1995; ). Epizootic hemorrhagic disease virus and bluetongue virus serotype distribution in white-tailed deer in Georgia. . J Wildl Dis 31:, 331–338. [CrossRef] [PubMed]
    [Google Scholar]
  25. Yadin H., Brenner J., Bumbrov V., Oved Z., Stram Y., Klement E., Perl S., Anthony S., Maan S.. & other authors ( 2008; ). Epizootic haemorrhagic disease virus type 7 infection in cattle in Israel. . Vet Rec 162:, 53–56. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.059659-0
Loading
/content/journal/jgv/10.1099/vir.0.059659-0
Loading

Data & Media loading...

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error