@article{mbs:/content/journal/jgv/10.1099/vir.0.059659-0, author = "Anbalagan, Srivishnupriya and Cooper, Elyse and Klumper, Pat and Simonson, Randy R. and Hause, Ben M.", title = "Whole genome analysis of epizootic hemorrhagic disease virus identified limited genome constellations and preferential reassortment", journal= "Journal of General Virology", year = "2014", volume = "95", number = "2", pages = "434-441", doi = "https://doi.org/10.1099/vir.0.059659-0", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.059659-0", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "Epizootic hemorrhagic disease virus (EHDV) is a Culicoides transmitted orbivirus that causes haemorrhagic disease in wild and domestic ruminants. A collection of 44 EHDV isolated from 2008 to 2012 was fully sequenced and analysed phylogenetically. Serotype 2 viruses were the dominant serotype all years except 2012 when serotype 6 viruses represented 63 % of the isolates. High genetic similarity (>94 % identity) between serotype 1 and 2 virus VP1, VP3, VP4, VP6, NS1, NS2 and NS3 segments prevented identification of reassortment events for these segments. Additionally, there was little genetic diversity (>96 % identity) within serotypes for VP2, VP5 and VP7. Preferential reassortment within the homologous serotype was observed for VP2, VP5 and VP7 segments for type 1 and type 2 viruses. In contrast, type 6 viruses were all reassortants containing VP2 and VP5 derived from an exotic type 6 with the remaining segments most similar to type 2 viruses. These results suggest that reassortment between type 1 and type 2 viruses requires conservation of the VP2, VP5 and VP7 segment constellation while type 6 viruses only require VP2 and VP5 and are restricted to type 2-lineage VP7. As type 6 VP2 and VP5 segments were exclusively identified in viruses with type 2-derived VP7, these results suggest functional complementation between type 2 and type 6 VP7 proteins.", }