1887

Abstract

Bean pod mottle virus (BPMV) is a bipartite, positive-sense (+) RNA plant virus of the family . Its RNA1 encodes all proteins needed for genome replication and is capable of autonomous replication. By contrast, BPMV RNA2 must utilize RNA1-encoded proteins for replication. Here, we sought to identify RNA elements in RNA2 required for its replication. The exchange of 5′ untranslated regions (UTRs) between genome segments revealed an RNA2-specific element in its 5′ UTR. Further mapping localized a 66 nucleotide region that was predicted to fold into an RNA stem–loop structure, designated SLC. Additional functional analysis indicated the importance of the middle portion of the stem and an adjacent two-base mismatch. This is the first report of a -acting RNA element in RNA2 of a bipartite secovirus.

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2013-06-01
2024-11-14
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References

  1. Baumstark T., Ahlquist P. 2001; The brome mosaic virus RNA3 intergenic replication enhancer folds to mimic a tRNA TψC-stem loop and is modified in vivo. RNA 7:1652–1670[PubMed]
    [Google Scholar]
  2. den Boon J. A., Ahlquist P. 2010; Organelle-like membrane compartmentalization of positive-strand RNA virus replication factories. Annu Rev Microbiol 64:241–256 [View Article][PubMed]
    [Google Scholar]
  3. Gaire F., Schmitt C., Stussi-Garaud C., Pinck L., Ritzenthaler C. 1999; Protein 2A of grapevine fanleaf nepovirus is implicated in RNA2 replication and colocalizes to the replication site. Virology 264:25–36 [View Article][PubMed]
    [Google Scholar]
  4. Gu H., Ghabrial S. A. 2005; The Bean pod mottle virus proteinase cofactor and putative helicase are symptom severity determinants. Virology 333:271–283 [View Article][PubMed]
    [Google Scholar]
  5. Hernandez-Garcia C. M., Bouchard R. A., Rushton P. J., Jones M. L., Chen X., Timko M. P., Finer J. J. 2010; High level transgenic expression of soybean (Glycine max) GmERF and Gmubi gene promoters isolated by a novel promoter analysis pipeline. BMC Plant Biol 10:237 [View Article][PubMed]
    [Google Scholar]
  6. Iwakawa H. O., Mine A., Hyodo K., An M., Kaido M., Mise K., Okuno T. 2011; Template recognition mechanisms by replicase proteins differ between bipartite positive-strand genomic RNAs of a plant virus. J Virol 85:497–509 [View Article][PubMed]
    [Google Scholar]
  7. Le Gall O., Christian P., Fauquet C. M., King A. M. Q., Knowles N. J., Nakashima N., Stanway G., Gorbalenya A. E. 2008; Picornavirales, a proposed order of positive-sense single-stranded RNA viruses with a pseudo-T  =  3 virion architecture. Arch Virol 153:715–727 [View Article][PubMed]
    [Google Scholar]
  8. Louime C., Vasanthaiah H. K. N., Jittayasothorn Y., Lu J., Basha S. M., Thipyapong P., Boonkerd N. 2008; A simple and efficient protocol for high quality RNA extraction and cloning of chalcone synthase partial CDS from muscadine grape cultivars (Vitis Rotundifolia Michx.). Eur J Sci Res 22:232–240
    [Google Scholar]
  9. MacFarlane S. A., Shanks M., Davies J. W., Zlotnick A., Lomonossoff G. P. 1991; Analysis of the nucleotide sequence of bean pod mottle virus middle component RNA. Virology 183:405–409 [View Article][PubMed]
    [Google Scholar]
  10. Nagy P. D., Pogany J. 2010; Global genomics and proteomics approaches to identify host factors as targets to induce resistance against Tomato bushy stunt virus. Adv Virus Res 76:123–177 [View Article][PubMed]
    [Google Scholar]
  11. Rohll J. B., Holness C. L., Lomonossoff G. P., Maule A. J. 1993; 3′-terminal nucleotide sequences important for the accumulation of cowpea mosaic virus M-RNA. Virology 193:672–679 [View Article][PubMed]
    [Google Scholar]
  12. Sanfaçon H., Wellink J., Le Gall O., Karasev A., van der Vlugt R., Wetzel T. 2009; Secoviridae: a proposed family of plant viruses within the order Picornavirales that combines the families Sequiviridae and Comoviridae, the unassigned genera Cheravirus and Sadwavirus, and the proposed genus Torradovirus. Arch Virol 154:899–907 [View Article][PubMed]
    [Google Scholar]
  13. Schwartz M., Chen J., Janda M., Sullivan M., den Boon J., Ahlquist P. 2002; A positive-strand RNA virus replication complex parallels form and function of retrovirus capsids. Mol Cell 9:505–514 [View Article][PubMed]
    [Google Scholar]
  14. van Bokhoven H., Le Gall O., Kasteel D., Verver J., Wellink J., Van Kammen A. B. 1993; Cis- and trans-acting elements in cowpea mosaic virus RNA replication. Virology 195:377–386 [View Article][PubMed]
    [Google Scholar]
  15. Wang J., Yeh H.-H., Falk B. W. 2009; cis preferential replication of Lettuce infectious yellows virus (LIYV) RNA 1: the initial step in the asynchronous replication of the LIYV genomic RNAs. Virology 386:217–223 [View Article][PubMed]
    [Google Scholar]
  16. Yi G., Kao C. 2008; cis- and trans-acting functions of brome mosaic virus protein 1a in genomic RNA1 replication. J Virol 82:3045–3053 [View Article][PubMed]
    [Google Scholar]
  17. Zhang C., Bradshaw J. D., Whitham S. A., Hill J. H. 2010; The development of an efficient multipurpose bean pod mottle virus viral vector set for foreign gene expression and RNA silencing. Plant Physiol 153:52–65 [View Article][PubMed]
    [Google Scholar]
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