1887

Abstract

Herpes simplex virus type 1 (HSV-1) triplex is a complex of three protein subunits, consisting of two copies of VP23 and one copy of VP19C. Here, we identified a non-classical NLS of VP19C between aa 50 and 61, and the nuclear import of VP19C was mediated by RanGTP and importin β1-, but not importin α5-, dependent pathway. Additionally, recombinant virus harbouring this NLS mutation (NLSm) replicates less efficiently as wild-type. These data strongly suggested that the nuclear import of VP19C is required for efficient HSV-1 production.

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2012-09-01
2020-07-07
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References

  1. Adamson W. E., McNab D., Preston V. G., Rixon F. J. 2006; Mutational analysis of the herpes simplex virus triplex protein VP19C. J Virol 80:1537–1548 [CrossRef][PubMed]
    [Google Scholar]
  2. Cai M., Wang S., Xing J., Zheng C. 2011; Characterization of the nuclear import and export signals, and subcellular transport mechanism of varicella-zoster virus ORF9. J Gen Virol 92:621–626 [CrossRef][PubMed]
    [Google Scholar]
  3. Cai M., Wang S., Long J., Zheng C. 2012; Probing of the nuclear import and export signals and subcellular transport mechanism of varicella-zoster virus tegument protein open reading frame 10. Med Microbiol Immunol (Berl) 201:103–111 [CrossRef][PubMed]
    [Google Scholar]
  4. Chowdhury S. I., Batterson W. 1994; Transinhibition of herpes simplex virus replication by an inducible cell-resident gene encoding a dysfunctional VP19c capsid protein. Virus Res 33:67–87 [CrossRef][PubMed]
    [Google Scholar]
  5. Guo H., Mao R., Block T. M., Guo J. T. 2010; Production and function of the cytoplasmic deproteinized relaxed circular DNA of hepadnaviruses. J Virol 84:387–396 [CrossRef][PubMed]
    [Google Scholar]
  6. Jarosinski K., Kattenhorn L., Kaufer B., Ploegh H., Osterrieder N. 2007; A herpesvirus ubiquitin-specific protease is critical for efficient T cell lymphoma formation. Proc Natl Acad Sci U S A 104:20025–20030 [CrossRef][PubMed]
    [Google Scholar]
  7. Kim H. S., Huang E., Desai J., Sole M., Pryce E. N., Okoye M. E., Person S., Desai P. J. 2011; A domain in the herpes simplex virus 1 triplex protein VP23 is essential for closure of capsid shells into icosahedral structures. J Virol 85:12698–12707 [CrossRef][PubMed]
    [Google Scholar]
  8. Lange A., Mills R. E., Lange C. J., Stewart M., Devine S. E., Corbett A. H. 2007; Classical nuclear localization signals: definition, function, and interaction with importin alpha. J Biol Chem 282:5101–5105 [CrossRef][PubMed]
    [Google Scholar]
  9. Li X., Zhu F. 2009; Identification of the nuclear export and adjacent nuclear localization signals for ORF45 of Kaposi’s sarcoma-associated herpesvirus. J Virol 83:2531–2539 [CrossRef][PubMed]
    [Google Scholar]
  10. Li M., Wang S., Cai M., Zheng C. 2011a; Identification of nuclear and nucleolar localization signals of pseudorabies virus (PRV) early protein UL54 reveals that its nuclear targeting is required for efficient production of PRV. J Virol 85:10239–10251 [CrossRef][PubMed]
    [Google Scholar]
  11. Li Y., Wang S., Zhu H., Zheng C. 2011b; Cloning of the herpes simplex virus type 1 genome as a novel luciferase-tagged infectious bacterial artificial chromosome. Arch Virol 156:2267–2272 [CrossRef][PubMed]
    [Google Scholar]
  12. Miyamoto Y., Hieda M., Harreman M. T., Fukumoto M., Saiwaki T., Hodel A. E., Corbett A. H., Yoneda Y. 2002; Importin alpha can migrate into the nucleus in an importin beta- and Ran-independent manner. EMBO J 21:5833–5842 [CrossRef][PubMed]
    [Google Scholar]
  13. Moore M. S., Blobel G. 1993; The GTP-binding protein Ran/TC4 is required for protein import into the nucleus. Nature 365:661–663 [CrossRef][PubMed]
    [Google Scholar]
  14. Okoye M. E., Sexton G. L., Huang E., McCaffery J. M., Desai P. 2006; Functional analysis of the triplex proteins (VP19C and VP23) of herpes simplex virus type 1. J Virol 80:929–940 [CrossRef][PubMed]
    [Google Scholar]
  15. Palacios I., Weis K., Klebe C., Mattaj I. W., Dingwall C. 1996; RAN/TC4 mutants identify a common requirement for snRNP and protein import into the nucleus. J Cell Biol 133:485–494 [CrossRef][PubMed]
    [Google Scholar]
  16. Pemberton L. F., Paschal B. M. 2005; Mechanisms of receptor-mediated nuclear import and nuclear export. Traffic 6:187–198 [CrossRef][PubMed]
    [Google Scholar]
  17. Person S., Desai P. 1998; Capsids are formed in a mutant virus blocked at the maturation site of the UL26 and UL26.5 open reading frames of herpes simplex virus type 1 but are not formed in a null mutant of UL38 (VP19C). Virology 242:193–203 [CrossRef][PubMed]
    [Google Scholar]
  18. Reid S. P., Valmas C., Martinez O., Sanchez F. M., Basler C. F. 2007; Ebola virus VP24 proteins inhibit the interaction of NPI-1 subfamily karyopherin alpha proteins with activated STAT1. J Virol 81:13469–13477 [CrossRef][PubMed]
    [Google Scholar]
  19. Rixon F. J., Addison C., McGregor A., Macnab S. J., Nicholson P., Preston V. G., Tatman J. D. 1996; Multiple interactions control the intracellular localization of the herpes simplex virus type 1 capsid proteins. J Gen Virol 77:2251–2260 [CrossRef][PubMed]
    [Google Scholar]
  20. Saad A. S. 2003; Wavelets filtering for classification of very noisy electron microscopic single particles images–application on structure determination of VP5-VP19C recombinant. BMC Struct Biol 3:9 [CrossRef][PubMed]
    [Google Scholar]
  21. Solé M., Perkins E. M., Frisancho A., Huang E., Desai P. 2007; The N terminus of the herpes simplex virus type 1 triplex protein, VP19C, cannot be detected on the surface of the capsid shell by using an antibody (hemagglutinin) epitope tag. J Virol 81:8367–8370 [CrossRef][PubMed]
    [Google Scholar]
  22. Spencer J. V., Newcomb W. W., Thomsen D. R., Homa F. L., Brown J. C. 1998; Assembly of the herpes simplex virus capsid: preformed triplexes bind to the nascent capsid. J Virol 72:3944–3951[PubMed]
    [Google Scholar]
  23. Tanaka M., Kagawa H., Yamanashi Y., Sata T., Kawaguchi Y. 2003; Construction of an excisable bacterial artificial chromosome containing a full-length infectious clone of herpes simplex virus type 1: viruses reconstituted from the clone exhibit wild-type properties in vitro and in vivo. J Virol 77:1382–1391 [CrossRef][PubMed]
    [Google Scholar]
  24. Tatman J. D., Preston V. G., Nicholson P., Elliott R. M., Rixon F. J. 1994; Assembly of herpes simplex virus type 1 capsids using a panel of recombinant baculoviruses. J Gen Virol 75:1101–1113 [CrossRef][PubMed]
    [Google Scholar]
  25. Tischer B. K., von Einem J., Kaufer B., Osterrieder N. 2006; Two-step red-mediated recombination for versatile high-efficiency markerless DNA manipulation in Escherichia coli. Biotechniques 40:191–197 [CrossRef][PubMed]
    [Google Scholar]
  26. Trus B. L., Booy F. P., Newcomb W. W., Brown J. C., Homa F. L., Thomsen D. R., Steven A. C. 1996; The herpes simplex virus procapsid: structure, conformational changes upon maturation, and roles of the triplex proteins VP19c and VP23 in assembly. J Mol Biol 263:447–462 [CrossRef][PubMed]
    [Google Scholar]
  27. Wang J. P., Bowen G. N., Zhou S., Cerny A., Zacharia A., Knipe D. M., Finberg R. W., Kurt-Jones E. A. 2012; Role of specific innate immune responses in herpes simplex virus infection of the central nervous system. J Virol 86:2273–2281 [CrossRef][PubMed]
    [Google Scholar]
  28. Wu F., Wang S., Xing J., Li M., Zheng C. 2012; Characterization of nuclear import and export signals determining the subcellular localization of WD repeat-containing protein 42A (WDR42A). FEBS Lett 586:1079–1085 [CrossRef][PubMed]
    [Google Scholar]
  29. Xing J., Wu F., Pan W., Zheng C. 2010; Molecular anatomy of subcellular localization of HSV-1 tegument protein US11 in living cells. Virus Res 153:71–81 [CrossRef][PubMed]
    [Google Scholar]
  30. Xing J., Wang S., Lin F., Pan W., Hu C. D., Zheng C. 2011a; Comprehensive characterization of interaction complexes of herpes simplex virus type 1 ICP22, UL3, UL4, and UL20.5. J Virol 85:1881–1886 [CrossRef][PubMed]
    [Google Scholar]
  31. Xing J., Wang S., Li Y., Guo H., Zhao L., Pan W., Lin F., Zhu H., Wang L.other authors 2011b; Characterization of the subcellular localization of herpes simplex virus type 1 proteins in living cells. Med Microbiol Immunol (Berl) 200:61–68 [CrossRef][PubMed]
    [Google Scholar]
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