RT Journal Article SR Electronic(1) A1 Li, You A1 Zhao, Lei A1 Wang, Shuai A1 Xing, Junji A1 Zheng, ChunfuYR 2012 T1 Identification of a novel NLS of herpes simplex virus type 1 (HSV-1) VP19C and its nuclear localization is required for efficient production of HSV-1 JF Journal of General Virology, VO 93 IS 9 SP 1869 OP 1875 DO https://doi.org/10.1099/vir.0.042697-0 PB Microbiology Society, SN 1465-2099, AB Herpes simplex virus type 1 (HSV-1) triplex is a complex of three protein subunits, consisting of two copies of VP23 and one copy of VP19C. Here, we identified a non-classical NLS of VP19C between aa 50 and 61, and the nuclear import of VP19C was mediated by RanGTP and importin β1-, but not importin α5-, dependent pathway. Additionally, recombinant virus harbouring this NLS mutation (NLSm) replicates less efficiently as wild-type. These data strongly suggested that the nuclear import of VP19C is required for efficient HSV-1 production., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.042697-0