1887

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Volume 93, Issue 1

The C terminus of lettuce mosaic potyvirus cylindrical inclusion helicase interacts with the viral VPg and with lettuce translation eukaryotic initiation factor 4E Free

Abstract

Recessive resistance to lettuce mosaic virus (LMV) is conferred in lettuce by the gene, encoding the eukaryotic translation initiation factor 4E (eIF4E). The C terminus of the viral cylindrical inclusion helicase (CI-Cter), together with the VPg, is involved directly in overcoming resistance. In this study, recombinant LMV VPg and CI-Cter proteins from wild-type or resistance-breaking isolates were expressed and purified from . The allelic forms of eIF4E from susceptible or resistant lettuce cultivars were produced similarly and these proteins were used in ELISA-based assays to demonstrate the binding of the various forms of LMV CI-Cter to both lettuce eIF4E and LMV VPg proteins. All combinations tested displayed significant and specific interactions, and the interaction between the C-terminal part of the LMV CI and eIF4E was confirmed in bimolecular fluorescence complementation assays. Higher interaction signals for both CI–eIF4E and CI–VPg were observed for LMV-E, indicating that the eIF4E interaction network involving CI and VPg appears to be stronger in the case of this resistance-breaking isolate. This could suggest the need for a minimal interaction threshold for infection success in resistant lettuce, but more precise measurement of the interaction parameters linking eIF4E, VPg and CI is needed in order to reinforce such a hypothesis.

  • Received:
  • Accepted:
  • Published Online:
© 2012 SGM
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2012-01-01
2024-05-06
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The C terminus of lettuce mosaic potyvirus cylindrical inclusion helicase interacts with the viral VPg and with lettuce translation eukaryotic initiation factor 4E
J. Gen. Virol. 93, 184 (2012); https://doi.org/10.1099/vir.0.035881-0
/content/journal/jgv/10.1099/vir.0.035881-0
/content/journal/jgv/10.1099/vir.0.035881-0
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