RT Journal Article SR Electronic(1) A1 Tavert-Roudet, G. A1 Abdul-Razzak, A. A1 Doublet, B. A1 Walter, J. A1 Delaunay, T. A1 German-Retana, S. A1 Michon, T. A1 Le Gall, O. A1 Candresse, T.YR 2012 T1 The C terminus of lettuce mosaic potyvirus cylindrical inclusion helicase interacts with the viral VPg and with lettuce translation eukaryotic initiation factor 4E JF Journal of General Virology, VO 93 IS 1 SP 184 OP 193 DO https://doi.org/10.1099/vir.0.035881-0 PB Microbiology Society, SN 1465-2099, AB Recessive resistance to lettuce mosaic virus (LMV) is conferred in lettuce by the mo1 gene, encoding the eukaryotic translation initiation factor 4E (eIF4E). The C terminus of the viral cylindrical inclusion helicase (CI-Cter), together with the VPg, is involved directly in overcoming mo1 resistance. In this study, recombinant LMV VPg and CI-Cter proteins from wild-type or resistance-breaking isolates were expressed and purified from Escherichia coli. The allelic forms of eIF4E from susceptible or resistant lettuce cultivars were produced similarly and these proteins were used in ELISA-based assays to demonstrate the in vitro binding of the various forms of LMV CI-Cter to both lettuce eIF4E and LMV VPg proteins. All combinations tested displayed significant and specific interactions, and the interaction between the C-terminal part of the LMV CI and eIF4E was confirmed in vivo in bimolecular fluorescence complementation assays. Higher interaction signals for both CI–eIF4E and CI–VPg were observed for LMV-E, indicating that the eIF4E interaction network involving CI and VPg appears to be stronger in the case of this resistance-breaking isolate. This could suggest the need for a minimal interaction threshold for infection success in resistant lettuce, but more precise measurement of the interaction parameters linking eIF4E, VPg and CI is needed in order to reinforce such a hypothesis., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.035881-0