1887

Abstract

The helper component-proteinase (HC-Pro) is a multifunctional protein found among potyviruses. With respect to its silencing suppressor function, small RNA binding appears to be the major activity of HC-Pro. HC-Pro could also exhibit other suppressor activities. HC-Pro may inhibit the Hua Enhancer 1 (HEN1) activity. There is indirect evidence showing that either transient or stable expression of HC-Pro in plants results in an increase of non-methylated small RNAs. Here, we demonstrated that recombinant (ZYMV) HC-Pro inhibited the methyltransferase activity of HEN1 . Moreover, we found that the HC-Pro mutant, which has lost small RNA-binding activity, inhibited HEN1 activity, while the truncated proteins and total soluble bacterial proteins did not. Using the ELISA-binding assay, we provided evidence that the HC-Pro wild-type and HC-Pro both bound to HEN1, with HC-Pro binding stronger than HC-Pro. Motif mapping analysis revealed that the amino acids located between positions 139 and 320 of ZYMV HC-Pro were associated with HEN1 interaction.

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2011-09-01
2020-01-22
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Supplements

vol. , part 9, pp. 2222 - 2226

Plasmid construction of N- and C-terminal deleted MBP:HA-HC-Pro

Analysis of the purified MBP:HA-HC-Pro fusion protein by SDS-PAGE

Primer sequences used for the generation of MBP:HA-HC-Pro mutants [Single PDF file](253 KB)



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