1887

Abstract

Human hepatitis E virus (HEV) is considered an emerging pathogen in industrialized countries. The aim of the present study was to contribute to the body of knowledge available on the molecular epidemiology of acute hepatitis E in Italy. Three sets of HEV-specific primers targeting the ORF1 and ORF2 were used to examine serum samples collected from acute hepatitis patients positive for anti-HEV IgG and/or IgM, between 2007 and 2010. Seventeen patients (39.5 %) tested HEV RNA-positive: 12 infections, due to genotype 1, were associated with travel to endemic areas (Bangladesh, India and Pakistan), while five infections, due to genotype 3, were presumably autochthonous. Risk factors identified in this group included exposure to raw seafood, pork liver sausages and wild boar. Results from the present study confirm that human HEV infection in Italy is caused by different genotypes, depending on whether the infection is travel-related or autochthonous.

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2011-07-01
2020-01-29
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References

  1. Aggarwal R. . ( 2011; ). Hepatitis E: historical, contemporary and future perspectives. . J Gastroenterol Hepatol 26: Suppl. 1 72–82. [CrossRef].[PubMed]
    [Google Scholar]
  2. Aggarwal R. , Kini D. , Sofat S. , Naik S. R. , Krawczynski K. . ( 2000; ). Duration of viraemia and faecal viral excretion in acute hepatitis E. . Lancet 356:, 1081–1082. [CrossRef].[PubMed]
    [Google Scholar]
  3. Alavian S. , Fallahian F. , Lankarani K. . ( 2009; ). Epidemiology of hepatitis E in Iran and Pakistan. . Hepat Mon 9:, 60–65.
    [Google Scholar]
  4. Bartolini B. , Garbuglia A. R. , Horejsh D. , Martini F. , Carletti F. , Capobianchi M. R. . ( 2007; ). Simultaneous control of DNA and RNA processing efficiency using a nucleic acid calibration set. . Biotechniques 42:, 452–456. [CrossRef].[PubMed]
    [Google Scholar]
  5. Cacopardo B. , Russo R. , Preiser W. , Benanti F. , Brancati G. , Nunnari A. . ( 1997; ). Acute hepatitis E in Catania (eastern Sicily) 1980–1994. The role of hepatitis E virus. . Infection 25:, 313–316. [CrossRef].[PubMed]
    [Google Scholar]
  6. Caprioli A. , Martelli F. , Ostanello F. , Di Bartolo I. , Ruggeri F. M. , Del Chiaro L. , Tolari F. . ( 2007; ). Detection of hepatitis E virus in Italian pig herds. . Vet Rec 161:, 422–423. [CrossRef].[PubMed]
    [Google Scholar]
  7. Clemente-Casares P. , Rodriguez-Manzano J. , Girones R. . ( 2009; ). Hepatitis E virus genotype 3 and sporadically also genotype 1 circulate in the population of Catalonia, Spain. . J Water Health 7:, 664–673. [CrossRef].[PubMed]
    [Google Scholar]
  8. Dalton H. R. , Thurairajah P. H. , Fellows H. J. , Hussaini H. S. , Mitchell J. , Bendall R. , Banks M. , Ijaz S. , Teo C. G. , Levine D. F. . ( 2007; ). Autochthonous hepatitis E in southwest England. . J Viral Hepat 14:, 304–309. [CrossRef].[PubMed]
    [Google Scholar]
  9. Di Bartolo I. , Martelli F. , Inglese N. , Pourshaban M. , Caprioli A. , Ostanello F. , Ruggeri F. M. . ( 2008; ). Widespread diffusion of genotype 3 hepatitis E virus among farming swine in Northern Italy. . Vet Microbiol 132:, 47–55. [CrossRef].[PubMed]
    [Google Scholar]
  10. Di Bartolo I. , Ponterio E. , Castellini L. , Ostanello F. , Ruggeri F. M. . ( 2011; ). Viral and antibody HEV prevalence in swine at slaughterhouse in Italy. . Vet Microbiol 149:, 330–338.[PubMed] [CrossRef]
    [Google Scholar]
  11. Fogeda M. , Avellón A. , Cilla C. G. , Echevarría J. M. . ( 2009; ). Imported and autochthonous hepatitis E virus strains in Spain. . J Med Virol 81:, 1743–1749. [CrossRef].[PubMed]
    [Google Scholar]
  12. Grieco A. , Miele L. , Gasbarrini G. , Grillo R. . ( 2001; ). Sporadic HEV hepatitis in Italy. . Gut 48:, 580. [CrossRef].[PubMed]
    [Google Scholar]
  13. Herremans M. , Bakker J. , Duizer E. , Vennema H. , Koopmans M. P. G. . ( 2007; ). Use of serological assays for diagnosis of hepatitis E virus genotype 1 and 3 infections in a setting of low endemicity. . Clin Vaccine Immunol 14:, 562–568. [CrossRef].[PubMed]
    [Google Scholar]
  14. Huang S. , Zhang X. , Jiang H. , Yan Q. , Ai X. , Wang Y. , Cai J. , Jiang L. , Wu T. et al. ( 2010; ). Profile of acute infectious markers in sporadic hepatitis E. . PLoS ONE 5:, e13560. [CrossRef].[PubMed]
    [Google Scholar]
  15. Kaba M. , Davoust B. , Marié J. L. , Colson P. . ( 2010; ). Detection of hepatitis E virus in wild boar (Sus scrofa) livers. . Vet J 186:, 259–261. [CrossRef].[PubMed]
    [Google Scholar]
  16. Labrique A. B. , Zaman K. , Hossain Z. , Saha P. , Yunus M. , Hossain A. , Ticehurst J. , Nelson K. E. . ( 2009; ). Population seroprevalence of hepatitis E virus antibodies in rural Bangladesh. . Am J Trop Med Hyg 81:, 875–881. [CrossRef].[PubMed]
    [Google Scholar]
  17. La Rosa G. , Pourshaban M. , Iaconelli M. , Spuri-Vennarucci V. , Muscillo M. . ( 2010; ). Molecular detection of hepatitis E virus from sewage. . Arch Virol 155:, 589–593.[PubMed] [CrossRef]
    [Google Scholar]
  18. Legrand-Abravanel F. , Thevenet I. , Mansuy J. M. , Saune K. , Vischi F. , Peron J. M. , Kamar N. , Rostaing L. , Izopet J. . ( 2009; ). Good performance of immunoglobulin M assays in diagnosing genotype 3 hepatitis E virus infections. . Clin Vaccine Immunol 16:, 772–774. [CrossRef].[PubMed]
    [Google Scholar]
  19. Lewis H. C. , Wichmann O. , Duizer E. . ( 2010; a). Transmission routes and risk factors for autochthonous hepatitis E virus infection in Europe: a systematic review. . Epidemiol Infect 138:, 145–166. [CrossRef].[PubMed]
    [Google Scholar]
  20. Lewis H. C. , Wichmann O. , Duizer E. . ( 2010; b). Transmission routes and risk factors for autochthonous hepatitis E virus infection in Europe: a systematic review. . Epidemiol Infect 138:, 145–166. [CrossRef].[PubMed]
    [Google Scholar]
  21. Martelli F. , Toma S. , Di Bartolo I. , Caprioli A. , Ruggeri F. M. , Lelli D. , Bonci M. , Ostanello F. . ( 2010; ). Detection of Hepatitis E Virus (HEV) in Italian pigs displaying different pathological lesions. . Res Vet Sci 88:, 492–496. [CrossRef].[PubMed]
    [Google Scholar]
  22. Masia G. , Orrù G. , Liciardi M. , Desogus G. , Coppola R. C. , Murru V. , Argiolas M. , Orrù G. . ( 2009; ). Evidence of hepatitis E virus (HEV) infection in human and pigs in Sardinia, Italy. . J Prev Med Hyg 50:, 227–231.[PubMed]
    [Google Scholar]
  23. Mathur P. , Arora N. K. , Panda S. K. , Kapoor S. K. , Jailkhani B. L. , Irshad M. . ( 2001; ). Sero-epidemiology of hepatitis E virus (HEV) in urban and rural children of North India. . Indian Pediatr 38:, 461–475.[PubMed]
    [Google Scholar]
  24. McCaustland K. A. , Bi S. , Purdy M. A. , Bradley D. W. . ( 1991; ). Application of two RNA extraction methods prior to amplification of hepatitis E virus nucleic acid by the polymerase chain reaction. . J Virol Methods 35:, 331–342. [CrossRef].[PubMed]
    [Google Scholar]
  25. Meng X. J. . ( 2010; ). Recent advances in hepatitis E virus. . J Viral Hepat 17:, 153–161 . [PubMed] [CrossRef]
    [Google Scholar]
  26. Mizuo H. , Yazaki Y. , Sugawara K. , Tsuda F. , Takahashi M. , Nishizawa T. , Okamoto H. . ( 2005; ). Possible risk factors for the transmission of hepatitis E virus and for the severe form of hepatitis E acquired locally in Hokkaido, Japan. . J Med Virol 76:, 341–349. [CrossRef].[PubMed]
    [Google Scholar]
  27. Mushahwar I. K. . ( 2008; ). Hepatitis E virus: molecular virology, clinical features, diagnosis, transmission, epidemiology, and prevention. . J Med Virol 80:, 646–658. [CrossRef].[PubMed]
    [Google Scholar]
  28. Panda S. K. , Thakral D. , Rehman S. . ( 2007; ). Hepatitis E virus. . Rev Med Virol 17:, 151–180. [CrossRef].[PubMed]
    [Google Scholar]
  29. Pavio N. , Meng X. J. , Renou C. . ( 2010; ). Zoonotic hepatitis E: animal reservoirs and emerging risks. . Vet Res 41:, 46. [CrossRef].[PubMed]
    [Google Scholar]
  30. Pelosi E. , Clarke I. . ( 2008; ). Hepatitis E: a complex and global disease. . Emerg Health Threats J 1:.
    [Google Scholar]
  31. Pina S. , Buti M. , Cotrina M. , Piella J. , Girones R. . ( 2000; ). HEV identified in serum from humans with acute hepatitis and in sewage of animal origin in Spain. . J Hepatol 33:, 826–833. [CrossRef].[PubMed]
    [Google Scholar]
  32. Renou C. , Moreau X. , Pariente A. , Cadranel J. F. , Maringe E. , Morin T. , Causse X. , Payen J. L. , Izopet J. et al. ( 2008; ). A national survey of acute hepatitis E in France. . Aliment Pharmacol Ther 27:, 1086–1093. [CrossRef].[PubMed]
    [Google Scholar]
  33. Renou C. , Nicand E. , Pariente A. , Cadranel J. F. , Pavio N. . ( 2009; ). Quand rechercher et comment diagnostiquer une hépatite E autochtone?. Gastroenterol Clin Biol 33: Suppl. F27–F35 (in French). [CrossRef].[PubMed]
    [Google Scholar]
  34. Rodriguez-Manzano J. , Miagostovich M. , Hundesa A. , Clemente-Casares P. , Carratala A. , Buti M. , Jardi R. , Girones R. , Hundesa A. et al. ( 2010; ). Analysis of the evolution in the circulation of HAV and HEV in eastern Spain by testing urban sewage samples. . J Water Health 8:, 346–354. [CrossRef].[PubMed]
    [Google Scholar]
  35. Romanò L. , Paladini S. , Tagliacarne C. , Canuti M. , Bianchi S. , Zanetti A. R. . ( 2011; ). Hepatitis E in Italy: a long-term prospective study. . J Hepatol 54:, 34–40. [CrossRef].[PubMed]
    [Google Scholar]
  36. Sadler G. J. , Mells G. F. , Shah N. H. , Chesner I. M. , Walt R. P. . ( 2006; ). UK acquired hepatitis E–an emerging problem?. J Med Virol 78:, 473–475. [CrossRef].[PubMed]
    [Google Scholar]
  37. Schielke A. , Sachs K. , Lierz M. , Appel B. , Jansen A. , Johne R. . ( 2009; ). Detection of hepatitis E virus in wild boars of rural and urban regions in Germany and whole genome characterization of an endemic strain. . Virol J 6:, 58. [CrossRef].[PubMed]
    [Google Scholar]
  38. Schlauder G. G. , Desai S. M. , Zanetti A. R. , Tassopoulos N. C. , Mushahwar I. K. . ( 1999; ). Novel hepatitis E virus (HEV) isolates from Europe: evidence for additional genotypes of HEV. . J Med Virol 57:, 243–251. [CrossRef].[PubMed]
    [Google Scholar]
  39. Shrestha S. M. , Shrestha S. , Tsuda F. , Nishizawa T. , Gotanda Y. , Takeda N. , Okamoto H. . ( 2003; ). Molecular investigation of hepatitis E virus infection in patients with acute hepatitis in Kathmandu, Nepal. . J Med Virol 69:, 207–214. [CrossRef].[PubMed]
    [Google Scholar]
  40. Teo C. G. . ( 2007; ). The two clinico-epidemiological forms of hepatitis E. . J Viral Hepat 14:, 295–297. [CrossRef].[PubMed]
    [Google Scholar]
  41. Zanetti A. R. , Schlauder G. G. , Romanò L. , Tanzi E. , Fabris P. , Dawson G. J. , Mushahwar I. K. . ( 1999; ). Identification of a novel variant of hepatitis E virus in Italy. . J Med Virol 57:, 356–360. [CrossRef].[PubMed]
    [Google Scholar]
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vol. , part 7, pp. 1617 - 1626

PCRs and primers used

Evolutionary divergences in the three analysed regions [Single PDF file](53 KB)



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