1887

Abstract

Human papillomavirus type 16 (HPV-16) genotype variants have been the subject of several investigations, but study participants have rarely been sampled more than once. In this study, among a cohort of human immunodeficiency virus (HIV)-infected adults, HPV-16 variants were investigated in samples collected concurrently from the anus and cervix, as well as in serial samples collected from the same anatomical site at 12-month intervals. HPV-16 variants in stored extracts of cervical and anal samples were determined from subjects with multiple visits and at least one sample positive for HPV-16. Seven polymorphic nucleotide positions within the E6 region were analysed by pyrosequencing to determine genotype variants. Of 364 samples examined, 176 anal and 39 cervical swabs from 84 different subjects yielded unequivocal sequences of eight major HPV-16 variants. Eight samples contained probable novel HPV-16 variants and in one sample two variants were detected. In eight out of 29 (27.6 %) anal–cervical sample pairs positive for HPV-16, discordant variants were found. From 57 anal and nine cervical sample series of HPV-16-positive samples, a change in HPV-16 variant status over time was seen in nine (13.6 %) instances (seven anal and two cervical) from eight different participants. Changes in HPV-16 variants in HIV-infected adults were seen most frequently when different anatomical sites were sampled, but were also observed over time.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.018663-0
2010-08-01
2019-11-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/91/8/2068.html?itemId=/content/journal/jgv/10.1099/vir.0.018663-0&mimeType=html&fmt=ahah

References

  1. Berumen, J., Ordoñez, R. M., Lazcano, E., Salmeron, J., Galvan, S. C., Estrada, R. A., Yumes, E., Garcia-Carranca, A., Gonzalez-Lira, G. & Madrigal-de-la Campa, A. ( 2001; ). Asian–American variants of human papillomavirus 16 and risk for cervical cancer: a case–control study. J Natl Cancer Inst 93, 1325–1330.[CrossRef]
    [Google Scholar]
  2. Chan, S. Y., Ho, L., Ong, C. K., Chow, V., Drescher, B., Dürst, M., ter Meulen, J., Villa, L., Luande, J. & other authors ( 1992; ). Molecular variants of human papillomavirus type 16 from four continents suggest ancient pandemic spread of the virus and its coevolution with humankind. J Virol 66, 2057–2066.
    [Google Scholar]
  3. Chopjitt, P., Ekalaksananan, T., Pientong, C., Kongyigyoes, B., Kleebkaow, P. & Charoensiri, N. ( 2008; ). Prevalence of human papillomavirus type 16 and its variants in abnormal squamous cervical cells in Northeast Thailand. Int J Infect Dis 13, 212–219.
    [Google Scholar]
  4. Da Costa, M. M., Hogeboom, C. J., Holly, E. A. & Palefsky, J. M. ( 2002; ). Increased risk of high-grade anal neoplasia associated with a human papillomavirus type 16 E6 sequence variant. J Infect Dis 185, 1229–1237.[CrossRef]
    [Google Scholar]
  5. de Villiers, E. M., Fauquet, C., Broker, T. R., Bernard, H. U. & zur Hausen, H. ( 2004; ). Classification of papillomaviruses. Virology 324, 17–27.[CrossRef]
    [Google Scholar]
  6. Emeny, R. T., Herron, J. R., Xi, L. F., Koutsky, L. A., Kiviat, N. B. & Wheeler, C. M. ( 1999; ). Comparison of variant-specific hybridization and single-strand conformational polymorphism methods for detection of mixed human papillomavirus type 16 variant infections. J Clin Microbiol 37, 3627–3633.
    [Google Scholar]
  7. Habis, A. H., Vernon, S. D., Lee, D. R., Verma, M. & Unger, E. R. ( 2004; ). Molecular quality of exfoliated cervical cells: implications for molecular epidemiology and biomarker discovery. Cancer Epidemiol Biomarkers Prev 13, 492–496.
    [Google Scholar]
  8. Ho, L., Chan, S. Y., Chow, V., Chong, T., Tay, S. K., Villa, L. L. & Bernard, H. U. ( 1991; ). Sequence variants of human papillomavirus type 16 in clinical samples permit verification and extension of epidemiological studies and construction of a phylogenetic tree. J Clin Microbiol 29, 1765–1772.
    [Google Scholar]
  9. Ho, L., Chan, S. Y., Burk, R. D., Das, B. C., Fujinaga, K., Icenogle, J. P., Kahn, T., Kiviat, N., Lancaster, W. & Mavromara-Nazos, P. ( 1993; ). The genetic drift of human papillomavirus type 16 is a means of reconstructing prehistoric viral spread and the movement of ancient human populations. J Virol 67, 6413–6423.
    [Google Scholar]
  10. Londesborough, P., Ho, L., Terry, G., Cuzick, J., Wheeler, C. & Singer, A. ( 1996; ). Human papillomavirus genotype as a predictor of persistence and development of high-grade lesions in women with minor cervical abnormalities. Int J Cancer 69, 364–368.[CrossRef]
    [Google Scholar]
  11. Nindl, I., Rindfleisch, K., Lotz, B., Schneider, A. & Dürst, M. ( 1999; ). Uniform distribution of HPV 16 E6 and E7 variants in patients with normal histology, cervical intraepithelial neoplasia and cervical cancer. Int J Cancer 82, 203–207.[CrossRef]
    [Google Scholar]
  12. Rajeevan, M. S., Swan, D. C., Nisenbaum, R. L., Lee, D. R., Vernon, S. D., Ruffin, M. T., Horowitz, I. R., Flowers, L. C., Kmak, D. & other authors ( 2005; ). Epidemiologic and viral factors associated with cervical neoplasia in HPV-16-positive women. Int J Cancer 115, 114–120.[CrossRef]
    [Google Scholar]
  13. Swan, D. C., Rajeevan, M. S., Tortolero-Luna, G., Follen, M., Tucker, R. A. & Unger, E. R. ( 2005; ). Human papillomavirus type 16 E2 and E6/E7 variants. Gynecol Oncol 96, 695–700.[CrossRef]
    [Google Scholar]
  14. Swan, D. C., Limor, J. R., Duncan, K. L., Rajeevan, M. S. & Unger, E. R. ( 2006; ). Human papillomavirus type 16 variant assignment by pyrosequencing. J Virol Methods 136, 166–170.[CrossRef]
    [Google Scholar]
  15. Tornesello, M. L., Duranturo, M. L., Salatiello, I., Buonguro, L., Losito, S., Botti, G., Stellato, G., Greggi, S., Piccoli, R. & other authors ( 2004; ). Analysis of human papillomavirus type-16 variants in Italian women with cervical intraepithelial neoplasia and cervical cancer. J Med Virol 74, 117–126.[CrossRef]
    [Google Scholar]
  16. Tornesello, M. L., Duraturo, M. L., Giorgi-Rossi, P., Sansone, M., Piccoli, R., Buonaguro, L. & Buonaguro, F. M. ( 2008; ). Human papillomavirus (HPV) genotypes and HPV16 variants in human immunodeficiency virus-positive Italian women. J Gen Virol 89, 1380–1389.[CrossRef]
    [Google Scholar]
  17. van Belkum, A., Juffermans, L., Schrauwen, L., van Doornum, G., Burger, M. & Quint, W. ( 1995; ). Genotyping human papillomavirus type 16 isolates from persistently infected promiscuous individuals and cervical neoplasia. J Clin Microbiol 33, 2957–2962.
    [Google Scholar]
  18. Xi, L. F., Critchlow, C. W., Wheeler, C. M., Koutsky, L. A., Galloway, D. A., Kuypers, J., Hughes, J. P., Hawes, S. E., Surawicz, C. & other authors ( 1998; ). Risk of anal carcinoma in situ in relation to human papillomavirus type 16 variants. Cancer Res 58, 3839–3844.
    [Google Scholar]
  19. Yamada, T., Wheeler, C. M., Halpern, A. L., Stewart, A. C., Hildesheim, A. & Jenison, S. A. ( 1995; ). Human papillomavirus type 16 variant lineages in United States populations characterized by nucleotide sequence analysis of the E6, L2 and L1 coding segments. J Virol 69, 7743–7753.
    [Google Scholar]
  20. Yamada, T., Manos, M. M., Peto, J., Greer, C. E., Munoz, N., Bosch, F. X. & Wheeler, C. M. ( 1997; ). Human papillomavirus type 16 sequence variation in cervical cancers: a worldwide perspective. J Virol 71, 2463–2472.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.018663-0
Loading
/content/journal/jgv/10.1099/vir.0.018663-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error