Human adenovirus type 8 (HAdV-8) and 37 (HAdV-37) cause epidemic keratoconjunctivitis (EKC) associated with community-acquired and nosocomial infections. The nucleotide sequences of the entire hexon and fiber genes of eight HAdV-8 and 26 HAdV-37 strains were analysed and the transition mutations in each gene were compared among strains. Compared with prototype strains, the hexon gene of HAdV-8 and -37 strains showed between two and seven and one and twelve variations at nine and 21 different positions, respectively. All of these, except one position in HAdV-37, were located in the conserved region 4 (C4). There were only three polymorphisms in the fiber gene of both HAdV-8 and HAdV-37, fewer than those in C4. The nucleotide sequence of HAdV-8 and -37 C4 might be readily modified during EKC epidemics.


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  1. Adrian, T., Wolf, U., Lauer, H. J. & Wigand, R.(1990). Restriction site mapping of adenovirus type 8 genome types. Res Virol 141, 611–624.[CrossRef] [Google Scholar]
  2. Aoki, K. & Tagawa, Y.(2002). A twenty-one year surveillance of adenoviral conjunctivitis in Sapporo, Japan. Int Ophthalmol Clin 42, 49–54.[CrossRef] [Google Scholar]
  3. Aoki, K., Ishiko, H., Konno, T., Shimada, Y., Hayashi, A., Kaneko, H., Ohguchi, T., Tagawa, Y., Ohno, S. & Yamazaki, S.(2008). Epidemic keratoconjunctivitis due to the novel hexon-chimeric-intermediate 22,37/H8 human adenovirus. J Clin Microbiol 46, 3259–3269.[CrossRef] [Google Scholar]
  4. Ariga, T., Shimada, Y., Shiratori, K., Ohgami, K., Yamazaki, S., Tagawa, Y., Kikuchi, M., Miyakita, Y., Fujita, K. & other authors(2005). Five new genome types of adenovirus type 37 caused epidemic keratoconjunctivitis in Sapporo, Japan, for more than 10 years. J Clin Microbiol 43, 726–732.[CrossRef] [Google Scholar]
  5. Arnberg, N., Mei, Y. & Wadell, G.(1997). Fiber genes of adenoviruses with tropism for the eye and the genital tract. Virology 227, 239–244.[CrossRef] [Google Scholar]
  6. Benkö, M., Harrach, B. & Russell, W. C.(2000). Family Adenoviridae. In Virus Taxonomy: Seventh Report of the International Committee on Taxonomy of Viruses, pp. 227–238. Edited by M. H. V. van Regenmortel, C. M. Fauquet, D. H. L. Bishop, E. B. Carstens, M. K. Estes, S. M. Lemon, J. Maniloff, M. A. Mayo, D. J. McGeoch, C. R. Pringle & R. B. Wickner. San Diego, CA: Academic Press.
  7. Casas, I., Avellon, A., Mosquera, M., Jabado, O., Echevarria, J. E., Campos, R. H., Rewers, M., Perez-Breña, P., Lipkin, W. I. & Palacios, G.(2005). Molecular identification of adenoviruses in clinical samples by analyzing a partial hexon genomic region. J Clin Microbiol 43, 6176–6182.[CrossRef] [Google Scholar]
  8. Chang, C., Sheu, M., Chern, C., Lin, K., Huang, W. & Chen, C.(2001). Epidemic keratoconjunctivitis caused by a new genotype of adenovirus type 8 (Ad8) – a chronological review of Ad8 in Southern Taiwan. Jpn J Ophthalmol 45, 160–166.[CrossRef] [Google Scholar]
  9. Crawford-Miksza, L. & Schnurr, D. P.(1996). Analysis of 15 adenovirus hexon proteins reveals the location and structure of seven hypervariable regions containing serotype-specific residues. J Virol 70, 1836–1844. [Google Scholar]
  10. de Jong, J. C., Wigand, R., Wadell, G., Keller, D., Muzerie, C. J., Wermenbol, A. G. & Schaap, G. J.(1981). Adenovirus 37: identification and characterization of a medically important new adenovirus type of subgroup D. J Med Virol 7, 105–118.[CrossRef] [Google Scholar]
  11. de Jong, T. A., Wermenbol, A. G., van der Avoort, H. G. & Wigand, R.(1988). Genome type analysis of adenovirus 37 isolates. J Med Virol 25, 77–83.[CrossRef] [Google Scholar]
  12. Ebner, K., Pinsker, W. & Lion, T.(2005). Comparative sequence analysis of the hexon gene in the entire spectrum of human adenovirus serotypes: phylogenetic, taxonomic, and clinical implications. J Virol 79, 12635–12642.[CrossRef] [Google Scholar]
  13. Fujimoto, T., Okafuji, T., Okafuji, T., Ito, M., Nukuzuma, S., Chikahira, M. & Nishio, O.(2004). Evaluation of a bedside immunochromatographic test for detection of adenovirus in respiratory samples, by comparison to virus isolation, PCR, and real-time PCR. J Clin Microbiol 42, 5489–5492.[CrossRef] [Google Scholar]
  14. Heim, A., Ebnet, C., Harste, G. & Pring-Akerblom, P.(2003). Rapid and quantitative detection of human adenovirus DNA by real-time PCR. J Med Virol 70, 228–239.[CrossRef] [Google Scholar]
  15. Higuchi, M., Nakazono, N., Ishii, K., Ohno, S., Matsuda, H., Fujii, S., Fujinaga, K. & Kondo, K.(1987). Antigenic and restriction endonuclease analyses of new adenovirus types 19 and 37 causing acute conjunctivitis. Jpn J Ophthalmol 31, 547–557. [Google Scholar]
  16. Ishii, K., Nakazono, N., Fujinaga, K., Fujii, S., Kato, M., Ohtsuka, H., Aoki, K., Chen, C. W., Lin, C. C. & other authors(1987). Comparative studies on etiology and epidemiology of viral conjunctivitis in three countries of East Asia – Japan, Taiwan and South Korea. Int J Epidemiol 16, 98–103.[CrossRef] [Google Scholar]
  17. Ishiko, H., Shimada, Y., Konno, T., Hayashi, A., Ohguchi, T., Tagawa, Y., Aoki, K., Ohno, S. & Yamazaki, S.(2008). A novel human adenovirus causing nosocomial infections of epidemic keratoconjunctivitis. J Clin Microbiol 46, 2002–2008.[CrossRef] [Google Scholar]
  18. Jawetz, E., Hanna, L. & Thygeson, P. A.(1959). Laboratory infection with Ad8. Am J Hyg 69, 13–20. [Google Scholar]
  19. Jin, X. H., Ishiko, H., Nguyen, T. H., Ohguchi, T., Akanuma, M., Aoki, K. & Ohno, S.(2006). Molecular epidemiology of adenoviral conjunctivitis in Hanoi, Vietnam. Am J Ophthalmol 142, 1064–1066.[CrossRef] [Google Scholar]
  20. Jones, M. S., II, Harrach, B., Ganac, R. D., Gozum, M. M., Dela Cruz, W. P., Riedel, B., Pan, C., Delwart, E. L. & Schnurr, D. P.(2007). New adenovirus species found in a patient presenting with gastroenteritis. J Virol 81, 5978–5984.[CrossRef] [Google Scholar]
  21. Kaneko, H., Maruko, I., Iida, T., Ohguchi, T., Aoki, K., Ohno, S. & Suzutani, T.(2008). The possibility of human adenovirus detection from the conjunctiva in asymptomatic cases during nosocomial infection. Cornea 27, 527–530.[CrossRef] [Google Scholar]
  22. Kaneko, H., Iida, T., Ishiko, H., Ohguchi, T., Ariga, T., Tagawa, Y., Aoki, K., Ohno, S. & Suzutani, T.(2009). Analysis of the complete genome sequence of epidemic keratoconjunctivitis-related human adenovirus type 8, 19, 37 and a novel serotype. J Gen Virol 90, 1471–1476.[CrossRef] [Google Scholar]
  23. Madisch, I., Harste, G., Pommer, H. & Heim, A.(2005). Phylogenetic analysis of the main neutralization and hemagglutination determinants of all human adenovirus prototypes as a basis for molecular classification and taxonomy. J Virol 79, 15265–15276.[CrossRef] [Google Scholar]
  24. Miura-Ochiai, R., Shimada, Y., Konno, T., Yamazaki, S., Aoki, K., Ohno, S., Suzuki, E. & Ishiko, H.(2007). Quantitative detection and rapid identification of human adenoviruses. J Clin Microbiol 45, 958–967.[CrossRef] [Google Scholar]
  25. Pring-Akerblom, P., Trijssenaar, F. E., Adrian, T. & Hoyer, H.(1999). Multiplex polymerase chain reaction for subgenus-specific detection of human adenoviruses in clinical samples. J Med Virol 58, 87–92.[CrossRef] [Google Scholar]
  26. Robinson, C. M., Shariati, F., Gillaspy, A. F., Dyer, D. W. & Chodosh, J.(2008). Genomic and bioinformatics analysis of human adenovirus type 37: new insights into corneal tropism. BMC Genomics 9, 213[CrossRef] [Google Scholar]
  27. Sarantis, H., Johnson, G., Brown, M., Petric, M. & Tellier, R.(2004). Comprehensive detection and serotyping of human adenoviruses by PCR and sequencing. J Clin Microbiol 42, 3963–3969.[CrossRef] [Google Scholar]
  28. Shimada, Y., Ariga, T., Tagawa, Y., Aoki, K., Ohno, S. & Ishiko, H.(2004). Molecular diagnosis of human adenoviruses D and E by a phylogeny-based classification method using a partial hexon sequence. J Clin Microbiol 42, 1577–1584.[CrossRef] [Google Scholar]
  29. Swenson, P. D., Wadell, G., Allard, A. & Hierholzer, J. C.(2003). Adenoviruses. In Manual of Clinical Microbiology, 8th edn, vol. 2, pp. 1404–1417. Edited by P. R. Murray, E. J. Baron, J. H. Jorgensen, M A. Pfaller & R. H. Yolken. Washington, DC: ASM Press.
  30. Tabery, H. M.(1995). Two outbreaks of adenovirus type 8 keratoconjunctivitis with different outcome. Acta Ophthalmol Scand 73, 358–360. [Google Scholar]
  31. Tanaka-Yokogui, K., Itoh, N., Usui, N., Takeuchi, S., Uchio, E., Aoki, K., Usui, M. & Ohno, S.(2001). New genome type of adenovirus serotype 19 causing nosocomial infections of epidemic keratoconjunctivitis in Japan. J Med Virol 65, 530–533.[CrossRef] [Google Scholar]
  32. Vainio, K., Borch, E. & Bruu, A. L.(2001). No sequence variation in part of the hexon and the fiber genes of adenovirus 8 isolated from patients with conjunctivitis or epidemic keratoconjunctivitis (EKC) in Norway during 1989 to 1996. J Clin Pathol 54, 558–561.[CrossRef] [Google Scholar]
  33. Wold, W. S. M. & Horwitz, M. S.(2007).Adenoviridae. In Fields Virology, 5th edn, pp. 2395–2436. Edited by D. M. Knipe & P. M. Howley. Philadelphia, PA: Lippincott Williams & Wilkins.

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