Breu Branco virus (BE AR 492347) was isolated from () mosquitoes captured in Tucuruí, Pará State, northern Brazil, in 1988. No cross-reactivity by complement-fixation tests was observed between Breu Branco virus and other known arboviruses. Results of electron microscopy and physicochemical tests suggested that Breu Branco virus may be a member of the family . In order to elucidate its taxonomic status, a comprehensive genetic characterization was conducted for Breu Branco virus and related strains (BE AR 494475 and BE AR 486204) that were also isolated from mosquitoes in the same area. This included full-length genome sequencing, determination of genetic traits and phylogenetic analysis. Breu Branco virus showed a similar genome organization to members of the genus , family . Genetically, Breu Branco virus was indistinguishable from strains BE AR 494475 and BE AR 486204. Phylogenetic analysis suggested that Breu Branco virus BE AR 492347 and its related strains constitute a novel species of the genus . Breu Branco virus is the first Brazilian orbivirus and the fifth orbivirus in the world to be sequenced fully.


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vol. , part 9, pp. 2183 – 2190

Specific primers used for full-length sequencing of the Breu Branco, BE AR 494475 and BE AR 486204 virus genomes.

Selected orbiviruses and other members of the family used for phylogenetic analysis.

Genetic relationships among Breu Branco virus and strains BE AR 494475 and BE AR 486204 by each genome segment.

Summary of the nucleotide and amino acid substitutions along the entire S9 ORF of Breu Branco, BE AR 494475 and BE AR 486204 viruses.

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