1887

Abstract

In most forms of prion diseases, blood is infectious, but detection by immunochemistry techniques of the only available marker of infection (the misfolded prion protein, PrP) in blood remains elusive. We developed a novel method for the detection of PrP in blood of prion-infected rodents based on the finding that PrP is associated with plasma exosomes. However, further purification of the exosomes on a sucrose gradient was necessary to remove plasma immunoglobulins, which interfere with PrP, masking its detection by immunochemistry. Finally, we report that about 20% of plasma infectivity is associated with exosomes.

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/content/journal/jgv/10.1099/vir.0.000117
2015-07-01
2019-10-17
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