@article{mbs:/content/journal/jgv/10.1099/vir.0.000117, author = "Properzi, Francesca and Logozzi, Mariantonia and Abdel-Haq, Hanin and Federici, Cristina and Lugini, Luana and Azzarito, Tommaso and Cristofaro, Ilaria and di Sevo, Daniela and Ferroni, Elena and Cardone, Franco and Venditti, Massimo and Colone, Marisa and Comoy, Emmanuel and Durand, Valérie and Fais, Stefano and Pocchiari, Maurizio", title = "Detection of exosomal prions in blood by immunochemistry techniques", journal= "Journal of General Virology", year = "2015", volume = "96", number = "7", pages = "1969-1974", doi = "https://doi.org/10.1099/vir.0.000117", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.000117", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "In most forms of prion diseases, blood is infectious, but detection by immunochemistry techniques of the only available marker of infection (the misfolded prion protein, PrPTSE) in blood remains elusive. We developed a novel method for the detection of PrPTSE in blood of prion-infected rodents based on the finding that PrPTSE is associated with plasma exosomes. However, further purification of the exosomes on a sucrose gradient was necessary to remove plasma immunoglobulins, which interfere with PrPTSE, masking its detection by immunochemistry. Finally, we report that about 20% of plasma infectivity is associated with exosomes.", }