1887

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Volume 105, Issue 5

Virus-vectoring thrips regulate the excessive multiplication of tomato spotted wilt virus using their antiviral immune responses No Access

Abstract

The tomato spotted wilt virus (TSWV) is a member of the family and has an negative/ambisense single-stranded RNA genome. and are known to be dominant pests in (hot pepper) and can cause damage to the plant either directly by feeding, or indirectly by transmitting TSWV in a persistent and propagative manner, resulting in serious economic damage. This study compared the immune responses of two different thrips species against TSWV infection by transcriptome analysis, which then allowed the assessment of antiviral responses using RNA interference (RNAi). Both adult thrips shared about 90 % of the transcripts in non-viruliferous conditions. Most signal components of the immune pathways were shared by these two thrips species, and their expression levels fluctuated differentially in response to TSWV infection at early immature stages. The functional assays using RNAi treatments indicated that the Toll and JAK/STAT pathways were associated with the antiviral responses, but the IMD pathway was not. The upregulation of dorsal switch protein one supported its physiological role in recognizing TSWV infection and triggering the eicosanoid biosynthetic pathway, which mediates melanization and apoptosis in thrips. In addition, the signal components of the RNAi pathways fluctuated highly after TSWV infection. Individual RNAi treatments specific to the antiviral signalling and response components led to significant increases in the TSWV amount in the thrips, causing virus-induced mortality. These findings suggest that immune signalling pathways leading to antiviral responses are operating in the thrips to regulate TSWV litres to prevent a fatal viral overload. This study also indicates the differential antiviral responses between the TSWV-transmitting and .

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): antiviral , Frankliniella , hot pepper , immunity , RNA-Seq , thrips and tomato spotted wilt virus
Funding
This study was supported by the:
  • National Research Foundation (KR) (Award 2022R1A2B5B03001792)
    • Principle Award Recipient: YonggyunKim
© 2024 The Authors
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/content/journal/jgv/10.1099/jgv.0.001984
2024-05-08
2024-05-19
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Virus-vectoring thrips regulate the excessive multiplication of tomato spotted wilt virus using their antiviral immune responses
J. Gen. Virol. 105, 001984 (2024); https://doi.org/10.1099/jgv.0.001984
/content/journal/jgv/10.1099/jgv.0.001984
/content/journal/jgv/10.1099/jgv.0.001984
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