1887

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Volume 105, Issue 1

Drug screening identified that handelin inhibits feline calicivirus infection by inhibiting HSP70 expression No Access

Abstract

Feline calicivirus (FCV) is considered one of the major pathogens of cats worldwide and causes upper respiratory tract disease in all cats. In some cats, infection is by a highly virulent strain of FCV (vs.-FCV), which can cause severe and fatal systemic disease symptoms. At present, few antiviral drugs are approved for clinical treatment against FCV. Therefore, there is an imminent need for effective FCV antiviral agents. Here, we used observed a cytopathic effect (CPE) assay to screen 1746 traditional Chinese medicine monomer compounds and found one that can effectively inhibit FCV replication, namely, handelin, with an effective concentration (EC) value of approximately 2.5 µM. Further study showed that handelin inhibits FCV replication via interference with heat shock protein 70 (HSP70), which is a crucial host factor and plays a positive role in regulating viral replication. Moreover, handelin and HSP70 inhibitors have broad-spectrum antiviral activity. These findings indicate that handelin is a potential candidate for the treatment of FCV infection and that HSP70 may be an important drug target.

  • Received:
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Keyword(s): handelin , antiviral effect , chrysanthemum , feline calicivirus and heat shock protein 70
Funding
This study was supported by the:
  • Basic and Applied Basic Research Foundation of Guangdong Province (Award 2020B0301030007)
    • Principle Award Recipient: GuiqingPeng
  • Science Fund for Distinguished Young Scholars of Hubei Province (Award 32125037)
    • Principle Award Recipient: ZhouShen
  • National Natural Science Foundation of China (Award 32102652,)
    • Principle Award Recipient: GuiqingPeng
© 2024 The Authors
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/content/journal/jgv/10.1099/jgv.0.001936
2024-01-04
2024-05-15
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Drug screening identified that handelin inhibits feline calicivirus infection by inhibiting HSP70 expression in vitro
J. Gen. Virol. 105, 001936 (2024); https://doi.org/10.1099/jgv.0.001936
/content/journal/jgv/10.1099/jgv.0.001936
/content/journal/jgv/10.1099/jgv.0.001936
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