1887

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Volume 104, Issue 10

Peste des petits ruminants virus non-structural V and C proteins interact with the NF-κB p65 subunit and modulate pro-inflammatory cytokine gene induction No Access

Abstract

Peste des petits ruminants virus (PPRV) is known to induce transient immunosuppression in infected small ruminants by modulating several cellular pathways involved in the antiviral immune response. Our study shows that the PPRV-coded non-structural proteins C and V can interact with the cellular NF-κB p65 subunit. The PPRV-C protein interacts with the transactivation domain (TAD) while PPRV-V interacts with the Rel homology domain (RHD) of the NF-κB p65 subunit. Both viral proteins can suppress the NF-κB transcriptional activity and NF-κB-mediated transcription of cellular genes. PPRV-V protein expression can significantly inhibit the nuclear translocation of NF-κB p65 upon TNF-α stimulation, whereas PPRV-C does not affect it. The NF-κB-mediated pro-inflammatory cytokine gene expression is significantly downregulated in cells expressing PPRV-C or PPRV-V protein. Our study provides evidence suggesting a role of PPRV non-structural proteins V and C in the modulation of NF-κB signalling through interaction with the NF-κB p65 subunit.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): NF-κB , p65 , peste des petits ruminants virus (PPRV) , PPRV-C and PPRV-V
Funding
This study was supported by the:
  • DST-SERB (Award (IPA/2021/000136-G))
    • Principle Award Recipient: RajeevKaul
  • Indian Council of Medical Research (Award CMR (3/1/3/JRF-2018/HRD-011{63288}))
    • Principle Award Recipient: YashChaudhary
  • University Grants Commission (Award UGC 536/(CSIR-UGC NET DEC 2017))
    • Principle Award Recipient: JuhiJain
  • University of Delhi (Award IoE/2021/12/FRP)
    • Principle Award Recipient: RajeevKaul
  • Department of Biotechnology, Ministry of Science and Technology, India (Award BT/PR39261/DRUG/134/93/2021)
    • Principle Award Recipient: RajeevKaul
  • ICAR - National Agricultural Science Fund (Award NASF/ABA-6021/2017-18)
    • Principle Award Recipient: RajeevKaul
© 2023 The Authors
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/content/journal/jgv/10.1099/jgv.0.001907
2023-10-13
2024-05-20
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Peste des petits ruminants virus non-structural V and C proteins interact with the NF-κB p65 subunit and modulate pro-inflammatory cytokine gene induction
J. Gen. Virol. 104, 001907 (2023); https://doi.org/10.1099/jgv.0.001907
/content/journal/jgv/10.1099/jgv.0.001907
/content/journal/jgv/10.1099/jgv.0.001907
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