1887

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Volume 104, Issue 10

MERS-CoV and SARS-CoV-2 membrane proteins are modified with polylactosamine chains Open Access

Abstract

Coronaviruses are positive-stranded RNA enveloped viruses. The helical nucleocapsid is surrounded by a lipid bilayer in which are anchored three viral proteins: the spike (S), membrane (M) and envelope (E) proteins. The M protein is the major component of the viral envelope and is believed to be its building block. The M protein of Middle East respiratory syndrome coronavirus (MERS-CoV) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) contains a short N-terminal domain with an N-glycosylation site. We investigated their N-glycosylation and show that polylactosamine chains are conjugated to SARS-CoV-2 and MERS-CoV M proteins in transfected and infected cells. Acidic residues present in the first transmembrane segments of the proteins are required for their glycosylation. No specific signal to specify polylactosamine conjugation could be identified and high mannose-conjugated protein was incorporated into virus-like particles.

  • Received:
  • Accepted:
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Keyword(s): coronavirus , membrane protein , N-linked glycosylation and polylactosamines
Funding
This study was supported by the:
  • Agence Nationale de la Recherche (Award ANR-22-0E15-0030-01)
    • Principle Award Recipient: SandrineBelouzard
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2023-10-06
2024-05-20
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MERS-CoV and SARS-CoV-2 membrane proteins are modified with polylactosamine chains
J. Gen. Virol. 104, 001900 (2023); https://doi.org/10.1099/jgv.0.001900
/content/journal/jgv/10.1099/jgv.0.001900
/content/journal/jgv/10.1099/jgv.0.001900
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