1887

Abstract

Mammalian orthoreovirus (MRV) has been identified in humans, livestock and wild animals; this wide host range allows individual MRV to transmit into multiple species. Although several interspecies transmission and genetic reassortment events of MRVs among humans, livestock and wildlife have been reported, the genetic diversity and geographic distribution of MRVs in Africa are poorly understood. In this study, we report the first isolation and characterization of MRVs circulating in a pig population in Zambia. In our screening, MRV genomes were detected in 19.7 % (29/147) of faecal samples collected from pigs by reverse transcription PCR. Three infectious MRV strains (MRV-85, MRV-96 and MRV-117) were successfully isolated, and their complete genomes were sequenced. Recombination analyses based on the complete genome sequences of the isolated MRVs demonstrated that MRV-96 shared the S3 segment with a different MRV isolated from bats, and that the L1 and M3 segments of MRV-117 originated from bat and human MRVs, respectively. Our results suggest that the isolated MRVs emerged through genetic reassortment events with interspecies transmission. Given the lack of information regarding MRVs in Africa, further surveillance of MRVs circulating among humans, domestic animals and wildlife is required to assess potential risk for humans and animals.

Funding
This study was supported by the:
  • Ayato Takada , AMED and the Japan International Cooperation Agency (JICA) within the framework of the Science and Technology Research Partnership for Sustainable Development (SATREPS) , (Award JP19jm0110019)
  • Hirofumi Sawa , the Japan Initiative for Global Research Network of Infectious Diseases (J-GRID) from the Japan Agency for Medical Research and Development (AMED) , (Award JP19fm0108008)
  • Hirofumi Sawa , the Scientific Research on Innovative Areas and International Group from MEXT/JSPS KAKENHI , (Award JP16K21723)
  • Hirofumi Sawa , the Scientific Research on Innovative Areas and International Group from MEXT/JSPS KAKENHI , (Award JP16H06429)
  • Hirofumi Sawa , the Scientific Research on Innovative Areas and International Group from MEXT/JSPS KAKENHI , (Award JP16H06431)
  • Hirofumi Sawa , the Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT)/Japan Society for the Promotion of Science (JSPS) KAKENHI , (Award JP16H05805)
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/content/journal/jgv/10.1099/jgv.0.001476
2020-07-24
2020-12-03
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