1887

Abstract

Garlic virus X (GarVX) ORF3 encodes a p11 protein, which contributes to virus cell-to-cell movement and forms granules on the endoplasmic reticulum (ER) in . Expression of p11 either from a binary vector, PVX or TMV induced ER stress and the unfolded protein response (UPR), as demonstrated by an increase in transcription of the ER luminal binding protein () and genes. UPR-related programmed cell death (PCD) was elicited by PVX : p11 or TMV : p11 in systemic infected leaves. Examination of p11 mutants with deletions of two transmembrane domains (TM) revealed that both were required for generating granules and for inducing necrosis. TRV-based VIGS was used to investigate the correlation between expression and p11-induced UPR-related PCD. Less necrosis was observed on local and systemic leaves of knockdown plants when infected with PVXp11, suggesting that plays an important role in the UPR-related PCD response to p11 in .

Loading

Article metrics loading...

/content/journal/jgv/10.1099/jgv.0.000460
2016-06-01
2021-07-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/97/6/1462.html?itemId=/content/journal/jgv/10.1099/jgv.0.000460&mimeType=html&fmt=ahah

References

  1. Adams M. J., Antoniw J. F., Bar-Joseph M., Brunt A. A., Candresse T., Foster G. D., Martelli G. P., Milne R. G., Zavriev S. K., Fauquet C. M. 2004; The new plant virus family Flexiviridae and assessment of molecular criteria for species demarcation. Arch Virol 149:1045–1060 [View Article][PubMed]
    [Google Scholar]
  2. Aguilar E., Allende L., Del Toro F. J., Chung B. N., Canto T., Tenllado F. 2015; Effects of elevated CO(2) and temperature on pathogenicity determinants and virulence of potato virus X /potyvirus-associated synergism. Mol Plant Microbe Interact 28:1364–1373 [View Article][PubMed]
    [Google Scholar]
  3. Chen J., Zheng H. Y., Antoniw J. F., Adams M. J., Chen J. P., Lin L. 2004; Detection and classification of allexiviruses from garlic in China. Arch Virol 149:435–445 [View Article][PubMed]
    [Google Scholar]
  4. Deng Y., Humbert S., Liu J. X., Srivastava R., Rothstein S. J., Howell S. H. 2011; Heat induces the splicing by IRE1 of a mRNA encoding a transcription factor involved in the unfolded protein response in Arabidopsis. Proc Natl Acad Sci U S A 108:7247–7252 [View Article][PubMed]
    [Google Scholar]
  5. Gao H., Brandizzi F., Benning C., Larkin R. M. 2008; A membrane-tethered transcription factor defines a branch of the heat stress response in Arabidopsis thaliana. Proc Natl Acad Sci U S A 105:16398–16403 [View Article]
    [Google Scholar]
  6. Jiang S., Lu Y., Li K., Lin L., Zheng H., Yan F., Chen J. 2014; Heat shock protein 70 is necessary for Rice stripe virus infection in plants. Mol Plant Pathol 15:907–917 [View Article][PubMed]
    [Google Scholar]
  7. Ju H. J., Samuels T. D., Wang Y. S., Blancaflor E., Payton M., Mitra R., Krishnamurthy K., Nelson R. S., Verchot-Lubicz J. 2005; The potato virus X TGBp2 movement protein associates with endoplasmic reticulum-derived vesicles during virus infection. Plant Physiol 138:1877–1895 [View Article][PubMed]
    [Google Scholar]
  8. Koizumi N., Martinez I. M., Kimata Y., Kohno K., Sano H., Chrispeels M. J. 2001; Molecular characterization of two Arabidopsis Ire1 homologs, endoplasmic reticulum-located transmembrane protein kinases. Plant Physiol 127:949–962 [View Article][PubMed]
    [Google Scholar]
  9. Lacorte C., Ribeiro S. G., Lohuis D., Goldbach R., Prins M. 2010; Potatovirus X and Tobacco mosaic virus-based vectors compatible with the gateway cloning system. J Virol Methods 164:7–13 [View Article][PubMed]
    [Google Scholar]
  10. Liu J. -X., Srivastava R., Che P., Howell S. H. 2007; Salt stress responses in Arabidopsis utilize a signal transduction pathway related to endoplasmic reticulum stress signaling. Plant J 51:897–909 [View Article]
    [Google Scholar]
  11. Liu J. X., Howell S. H. 2010; bZIP28 and NF-Y transcription factors are activated by ER stress and assemble into a transcriptional complex to regulate stress response genes in Arabidopsis. Plant Cell 22:782–796 [View Article]
    [Google Scholar]
  12. Liu Y., Schiff Y., Marathe Y., Dinesh-Kumar Y. 2002; Tobacco Rar1, EDS1 and NPR1/NIM1 like genes are required for N-mediated resistance to tobacco mosaic virus. Plant J 30:415–429 [View Article][PubMed]
    [Google Scholar]
  13. Lu Y., Yan F., Guo W., Zheng H., Lin L., Peng J., Adams M. J., Chen J. 2011; Garlic virus X 11-kDa protein granules move within the cytoplasm and traffic a host protein normally found in the nucleolus. Mol Plant Pathol 12:666–676 [View Article][PubMed]
    [Google Scholar]
  14. Martínez I. M., Chrispeels M. J. 2003; Genomic analysis of the unfolded protein response in Arabidopsis shows its connection to important cellular processes. Plant Cell 15:561–576 [View Article][PubMed]
    [Google Scholar]
  15. Moreno A. A., Mukhtar M. S., Blanco F., Moreno I., Jordan M. R., Chen Y., Brandizzi F., Dong X, Boatwright J. L., other authors. 2012; IRE1/bZIP60-mediated unfolded protein response plays distinct roles in plant immunity and abiotic stress responses. PloS One 7:e31944–e31944 [View Article]
    [Google Scholar]
  16. Nagashima Y., Mishiba K., Suzuki E., Shimada Y., Iwata Y., Koizumi N. 2011; Arabidopsis IRE1 catalyses unconventional splicing of bZIP60 mRNA to produce the active transcription factor. Sci Rep 1:29 [View Article][PubMed]
    [Google Scholar]
  17. Nam M., Lee Y. H., Park C. Y., Lee M. A., Bae Y. S., Lim S., Lee J. H., Moon J. S., Lee S. H. 2015; Development of multiplex RT-PCR for simultaneous detection of garlic viruses and the incidence of garlic viral disease in garlic genetic resources. Plant Patho J 31:90–96 [View Article][PubMed]
    [Google Scholar]
  18. Peng J., Yang J., Yan F., Lu Y., Jiang S., Lin L., Zheng H., Chen H., Chen J. 2011; Silencing of NbXrn4 facilitates the systemic infection of Tobacco mosaic virus in Nicotiana benthamiana. Virus Res 158:268–270 [View Article][PubMed]
    [Google Scholar]
  19. Sitia R., Braakman I. 2003; Quality control in the endoplasmic reticulum protein factory. Nat New Biol 426:891–894 [View Article]
    [Google Scholar]
  20. Song S. I., Song J. T., Kim C. H., Lee J. S., Choi Y. D. 1998; Molecular characterization of the garlic virus X genome. J Gen Virol 79 (Pt 1):155–159 [View Article][PubMed]
    [Google Scholar]
  21. Sun Z., Yang D., Xie L., Sun L., Zhang S., Zhu Q., Li J., Wang X., Chen J. 2013; Rice black-streaked dwarf virus P10 induces membranous structures at the ER and elicits the unfolded protein response in Nicotiana benthamiana. Virology 447:131–139 [View Article]
    [Google Scholar]
  22. Tateda C., Ozaki R., Onodera Y., Takahashi Y., Yamaguchi K., Berberich T., Koizumi N., Kusano T. 2008; NtbZIP60, an endoplasmic reticulum-localized transcription factor, plays a role in the defense response against bacterial pathogens in Nicotiana tabacum. J Plant Res 121:603–611 [View Article][PubMed]
    [Google Scholar]
  23. Urade R. 2007; Cellular response to unfolded proteins in the endoplasmic reticulum of plants. FEBS J 274:1152–1171 [View Article][PubMed]
    [Google Scholar]
  24. Williams B., Verchot J., Dickman M. B. 2014; When supply does not meet demand-ER stress and plant programmed cell death. Front Plant Sci 5:211 [View Article][PubMed]
    [Google Scholar]
  25. Wylie S. J., Li H., Jones M. G. K. 2011; Phylogenetic analysis of allexiviruses identified on garlic from australia. Australasian Plant Disease Notes 7:23–27 [View Article]
    [Google Scholar]
  26. Wylie S. J., Li H., Saqib M., Jones M. G. 2014; The global trade in fresh produce and the vagility of plant viruses: a case study in garlic. Plos One 9:e105044 [View Article][PubMed]
    [Google Scholar]
  27. Ye C.-M., Chen S., Payton M., Dickman M. B., Verchot J. 2013; TGBp3 triggers the unfolded protein response and SKP1-dependent programmed cell death. Mol Plant Pathol 14:241–255 [View Article]
    [Google Scholar]
  28. Ye C., Dickman M. B., Whitham S. A., Payton M., Verchot J. 2011; The unfolded protein response is triggered by a plant viral movement protein. Plant Physiol 156:741–755 [View Article]
    [Google Scholar]
  29. Yu C.-Y., Hsu Y.-W., Liao C.-L., Lin Y.-L. 2006; Flavivirus infection activates the XBP1 pathway of the unfolded protein response to cope with endoplasmic reticulum stress. J Virol 80:11868–11880 [View Article]
    [Google Scholar]
  30. Zhang L., Chen H., Brandizzi F., Verchot J., Wang A. 2015; The UPR branch IRE1-bZIP60 in plants plays an essential role in viral infection and is complementary to the only UPR pathway in yeast. PLoS Genet 11:e1005164 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/jgv.0.000460
Loading
/content/journal/jgv/10.1099/jgv.0.000460
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error