1887

Abstract

To investigate the immune response to anti-rabies vaccination in the principal recipient (the domestic dog), four truncated fragments of the rabies virus glycoprotein were expressed as glutathione -transferase fusion proteins. Immune sera from vaccinated rabbits and dogs were then used to probe for reactivity with these expressed proteins. In two rabbits and four dogs tested, the dominant antibody response to non-conformational antigenic sites appeared to be directed to a region of the glycoprotein between amino acids 222 and 332. The N-terminal fragment of the glycoprotein was also significantly antigenic. Further studies to assess whether the antibody response to the internal domain could neutralize the rabies Challenge Virus Standard (CVS) strain, using antibody depletion, suggested that this fraction did contribute to the ability of post-vaccination sera to neutralize and therefore protect against infection.

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2002-11-01
2020-10-28
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References

  1. Anilionis A., Wunner W. H., Curtis P. J.. 1981; Structure of the glycoprotein gene in rabies virus. Nature294:275–278
    [Google Scholar]
  2. Badrane H., Bahloul C., Perrin P., Tordo N.. 2001; Evidence for two lyssavirus phylogroups with distinct pathogenicity and immunogenicity. Journal of Virology75:3268–3276
    [Google Scholar]
  3. Bunschoten H., Gore M., Claassen I. J. T. M., Uytdehaag F. G. C. M., Dietzschold B., Wunner W. H., Osterhaus A. D. M. E.. 1989; Characterisation of a new virus-neutralizing epitope that denotes a sequential determinant on the rabies virus glycoprotein. Journal of General Virology70:291–298
    [Google Scholar]
  4. Champion J. M., Kean R. B., Rupprecht C. E., Notkins A. L., Koprowski H., Dietzschold B., Hooper D. C.. 2000; The development of monoclonal human rabies-neutralizing antibodies as a substitute for pooled human immune globulin in the prophylactic treatment of rabies virus exposure. Journal of Immunological Methods231:81–90
    [Google Scholar]
  5. Cliquet F., Aubert M., Sagne L.. 1998; Development of a fluorescent antibody virus neutralisation test (FAVN) for the quantification of rabies-neutralizing antibody. Journal of Immunological Methods212:79–87
    [Google Scholar]
  6. Coll J. M.. 1995; The glycoprotein G of rhabdoviruses. Archives of Virology140:827–851
    [Google Scholar]
  7. Coulon P., Derbin C., Kucera P., Lafay F., Prehaud C., Flamand A.. 1989; Invasion of the peripheral nervous systems of adult mice by the CVS strain of rabies virus and its avirulent derivative AvO1. Journal of Virology63:3550–3554
    [Google Scholar]
  8. Coulon P., Lafay F., Flamand A.. 1993; Rabies virus antigenicity: an overview. Onderstepoort Journal of Veterinary Virology60:271–275
    [Google Scholar]
  9. Dietzschold B., Wiktor T. J., Macfarlan R., Varrichio A.. 1982; Antigenic structure of rabies virus glycoprotein: ordering and immunological characterization of the large CNBr cleavage fragments. Journal of Virology44:595–602
    [Google Scholar]
  10. Dietzschold B., Wunner W. H., Tadeusz J. W., Lopes D., Lafon M., Smith C. L., Koprowski H.. 1983; Characterization of an antigenic determinant of the rabies glycoprotein that correlates with pathogenicity of rabies virus. Proceedings of the National Academy of Sciences, USA80:70–74
    [Google Scholar]
  11. Dietzschold B., Gore M., Marchadier D., Niu H. S., Bunschoten H. M., Otvos L. Jr, Wunner W. H., Ertl H. C. J., Osterhaus A. D. M. E., Koprowski H.. 1990; Structural and immunological characterization of a linear virus-neutralizing epitope of the rabies virus glycoprotein and its possible use in a synthetic vaccine. Journal of Virology64:3804–3809
    [Google Scholar]
  12. Fooks T., McElhinney L. M., Brookes S. M., Johnson N., Keene V., Parsons G., Soldan A.. 2002; Rabies antibody testing and the UK Pet Travel Scheme. Veterinary Record150:428–430
    [Google Scholar]
  13. Gaudin Y., Ruigrok R. W. H., Tuffereau C., Knossow M., Flamand A.. 1992; Rabies virus glycoprotein is a trimer. Virology184:441–444
    [Google Scholar]
  14. Heaton P. R., Johnstone P., McElhinney L. M., Cowley R., O′Sullivan E., Whitby J. E.. 1997; Heminested PCR assay for detection of six genotypes of rabies and rabies-related viruses. Journal of Clinical Microbiology35:2762–2766
    [Google Scholar]
  15. Hemachuda T., Phuapradit P.. 1997; Rabies. Current Opinion in Neurobiology10:260–267
    [Google Scholar]
  16. Hooper D. C., Morimoto K., Bette M., Weihem E., Koprowski H., Dietzschold B.. 1998; Collaboration of antibody and inflammation in clearance of rabies virus from the central nervous system. Journal of Virology72:3711–3719
    [Google Scholar]
  17. Lafay F., Benmansour A., Chebli K., Flamand A.. 1996; Immunodominant epitopes defined by a yeast-expressed library of random fragments of the rabies virus glycoprotein map outside major antigenic sites. Journal of General Virology77:339–346
    [Google Scholar]
  18. Luo T. R., Minamoto N., Ito H., Goto H., Hiraga S., Ito N., Sugiyama M., Kinjo T.. 1997; A virus-neutralizing epitope on the glycoprotein of rabies virus that contains Trp251 is a linear epitope. Virus Research51:35–41
    [Google Scholar]
  19. Ni Y., Tominaga Y., Honda Y., Morimoto K., Sakamoto S., Kawai A.. 1995; Mapping and characterization of a sequential epitope on the rabies virus glycoprotein which is recognized by a neutralizing monoclonal antibody Rg719. Microbiology and Immunology39:693–702
    [Google Scholar]
  20. Prehaud C., Coulon P., Lafay F., Thiers C., Flamand A.. 1988; Antigenic site II of rabies virus glycoprotein: structure and role in viral virulence. Journal of Virology62:1–7
    [Google Scholar]
  21. Seif I., Coulon P., Rollin P. E., Flamand A.. 1985; Rabies virulence: effect on pathogenicity and sequence characterization of rabies virus mutations affecting antigenic site III of the glycoprotein. Journal of Virology53:926–934
    [Google Scholar]
  22. Van der Heijden R. W. J., Langedijk J. P., Groen J., UytdeHaag F. G., Meloen R. H., Osterhaus A. D.. 1993; Structural and functional studies on a unique linear neutralizing antigenic site (G5) of the rabies virus glycoprotein. Journal of General Virology74:1539–1545
    [Google Scholar]
  23. Walker P. J., Kongsuwan K.. 1999; Deduced structural model for animal rhabdovirus glycoproteins. Journal of General Virology80:1211–1220
    [Google Scholar]
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