1887

Abstract

A transcription system for Toscana virus (TOSV) (a member of the family , genus ) was constructed. For expression, the TOSV transcription system uses the viral N and L proteins and an S-like RNA genome containing the chloramphenicol acetyltransferase reporter gene in the antisense orientation flanked by the viral genomic 5′- and 3′-terminal S sequences. It was found that the N and L proteins represent the minimal protein requirement for an active transcription complex. To investigate the possibility of reassortment between TOSV and Rift Valley fever virus (RVFV), the activity of their polymerase complexes was tested on their heterologous S-like RNA genomes and this showed that both virus complexes were active. Moreover, hybrid transcriptase complexes with protein components originating from the two viruses were tested on both virus templates and only the combination RVFV L + TOSV N on RVFV S-like RNA was found to be active in this assay. These results suggest that virus reassortants might be generated whenever the two viruses infect the same host.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-82-4-781
2001-04-01
2020-01-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/82/4/0820781a.html?itemId=/content/journal/jgv/10.1099/0022-1317-82-4-781&mimeType=html&fmt=ahah

References

  1. Accardi, L., Grò, M. C., Di Bonito, P. & Giorgi, C. ( 1993; ). Toscana virus genomic L segment: molecular cloning, coding strategy and amino acid sequence in comparison with other negative strand RNA viruses. Virus Research 27, 119-131.[CrossRef]
    [Google Scholar]
  2. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. & Struhl, K. (1995). Current Protocols in Molecular Biology. New York: John Wiley.
  3. Bouloy, M., Jansen, C., Vialat, P., Khun, H., Pavlovic, J., Huerke, M. & Haller, O. ( 2001; ). Genetic evidence for an interferon antagonist effect of the Rift Valley fever virus non-structural protein NSs. Journal of Virology 75, 1371–1377.[CrossRef]
    [Google Scholar]
  4. Di Bonito, P., Nicoletti, L., Mochi, S., Accardi, L., Marchi, A. & Giorgi, C. ( 1999; ). Immunological characterization of Toscana virus proteins. Archives of Virology 144, 1947-1960.[CrossRef]
    [Google Scholar]
  5. Dunn, E. F., Pritlove, D. C., Jin, H. & Elliott, R. M. ( 1995; ). Transcription of a recombinant Bunyavirus RNA template by transiently expressed Bunyavirus proteins. Virology 211, 133-143.[CrossRef]
    [Google Scholar]
  6. Elliott, R. M. ( 1996; ). The Bunyaviridae. Concluding remarks and future prospects. In The Bunyaviridae , pp. 295-332. Edited by R. M. Elliott. New York:Plenum Press.
  7. Giorgi, C. ( 1996; ). Molecular biology of Phleboviruses. In The Bunyaviridae , pp. 105-128. Edited by R. M. Elliott. New York:Plenum Press.
  8. Giorgi, C., Accardi, L., Nicoletti, L., Grò, M. C., Takehara, K., Hilditch, C. & Bishop, D. H. ( 1991; ). Sequences and coding strategies of the S RNAs of Toscana and Rift Valley fever viruses compared to those of Punta Toro, Sicilian Sandfly fever, and Uukuniemi viruses. Virology 180, 738-753.[CrossRef]
    [Google Scholar]
  9. Haseloff, J. & Gerlach, W. L. ( 1988; ). Simple RNA enzymes with new and highly specific endoribonuclease activities. Nature 334, 585-591.[CrossRef]
    [Google Scholar]
  10. Jin, H. & Elliott, R. M. ( 1991; ). Expression of functional Bunyamwera virus L protein by recombinant vaccinia viruses. Journal of Virology 65, 4182-4189.
    [Google Scholar]
  11. Lopez, N., Müller, R., Prehaud, C. & Bouloy, M. ( 1995; ). The L protein of Rift Valley fever virus can rescue viral ribonucleoproteins and transcribe synthetic genome-like RNA molecules. Journal of Virology 69, 3972-3979.
    [Google Scholar]
  12. Müller, R., Poch, O., Delarue, M., Bishop, D. H. L. & Bouloy, M. ( 1994; ). Rift Valley fever virus L segment: correction of the sequence and possible functional role of newly identified regions conserved in RNA-dependent polymerases. Journal of General Virology 75, 1345-1352.[CrossRef]
    [Google Scholar]
  13. Nichol, S. T. (2000). Evolution of viruses in the real word. Genetics, Pathogenesis and Ecology of Emerging Viral Diseases (Taos, New Mexico, USA, 24–30 January, 2000).
  14. Nicoletti, L., Ciufolini, M. G. & Verani, P. ( 1996; ). Sandfly fever viruses in Italy. Archives of Virology Supplementum 11, 41-47.
    [Google Scholar]
  15. Pattnaik, A. K., Ball, L. A., LeGrone, A. W. & Wertz, G. W. ( 1992; ). Infectious defective interfering particles of VSV from transcripts of a cDNA clone. Cell 69, 1011-1020.[CrossRef]
    [Google Scholar]
  16. Perrotta, A. T. & Been, M. D. ( 1991; ). A pseudoknot-like structure required for efficient self cleavage of hepatitis delta virus RNA. Nature 350, 434-436.[CrossRef]
    [Google Scholar]
  17. Peters, C. J. & Meegan, J. M. ( 1989; ). Rift Valley fever. In CRC Handbook Series in Zoonoses , pp. 403-420. Edited by G. Beran. Boca Raton, FL:CRC Press.
  18. Prehaud, C., Lopez, N., Blok, M. J., Obry, V. & Bouloy, M. ( 1997; ). Analysis of the 3′ terminal sequence recognized by the Rift Valley fever virus transcription complex in its ambisense S segment. Virology 227, 189-197.[CrossRef]
    [Google Scholar]
  19. Pringle, C. R. ( 1996; ). Genetics and genome segment reassortment. In The Bunyaviridae , pp. 189-226. Edited by R. M. Elliott. Plenum Press:New York.
  20. Sall, A. A., Zanotto, P. M., Sene, O. K., Zeller, H. G., Digoutte, J. P., Thiongane, Y. & Bouloy, M. ( 1999; ). Genetic reassortment of Rift Valley fever virus in nature. Journal of Virology 73, 8196-8200.
    [Google Scholar]
  21. Sall, A. A., Zanotto, P. M., Vialat, P., Sene, O. K. & Bouloy, M. ( 1998; ). Origin of 1997–98 Rift Valley fever outbreak in East Africa. Lancet 352, 1596-1597.[CrossRef]
    [Google Scholar]
  22. Weber, F., Bridgen, A. & Elliott, R. M. (2000). The Bunyamwera virus non-structural protein NSs is a repressor of the viral polymerase and confers interferon antagonistic function. 11th International Conference on Negative Strand Viruses. (Quebec City, Canada, June 24–29, 2000).
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-82-4-781
Loading
/content/journal/jgv/10.1099/0022-1317-82-4-781
Loading

Data & Media loading...

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error